Engineered myoglobin as a catalyst for atom transfer radical cyclisation DOI Creative Commons
Andriy Lubskyy, Chao Guo, Robert J. Chadwick

et al.

Chemical Communications, Journal Year: 2022, Volume and Issue: 58(78), P. 10989 - 10992

Published: Jan. 1, 2022

Myoglobin was subjected to site-directed mutagenesis and transformed into a catalyst able perform atom transfer radical cyclisation reactions,

Language: Английский

Visible Light Photoiniferter Polymerization for Dispersity Control in High Molecular Weight Polymers DOI

Qingchi Ma,

Greg G. Qiao, Zesheng An

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(48)

Published: Oct. 10, 2023

Abstract The synthesis of polymers with high molecular weights, controlled sequence, and tunable dispersities remains a challenge. A simple effective visible‐light photoiniferter reversible addition‐fragmentation chain transfer (RAFT) polymerization is reported here to realize this goal. Key strategy the use switchable RAFT agents (SRAs) tune activities coupled inherent highly living nature polymerization. SRAs were in situ adjusted by addition acid. In chain‐transfer coefficient, photolysis kinetic studies revealed that neutral protonated showed different rates, which unique terms dispersity control. This features no catalyst, exogenous radical source, temporal regulation visible light, unprecedented weight regime (up 500 kg mol −1 ). Pentablock copolymers three combinations also synthesized, highlighting was maintained even for blocks large dispersities. T g lowered high‐dispersity similar MWs due existence more low‐MW polymers. holds great potential advanced materials weight, sequence.

Language: Английский

Citations

11
An electrochemical biosensor for the amplification of thrombin activity by perylene-mediated photoinitiated polymerization DOI

Shuaibing Yu,

Jingliang Liu, Lianzhi Li

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1302, P. 342494 - 342494

Published: March 20, 2024

Language: Английский

Citations

4
Green synthesis of polymer materials via enzyme- initiated RAFT polymerization DOI
Sonu Kumar,

Raviteja Gaddala,

Sabu Thomas

et al.

Polymer Chemistry, Journal Year: 2024, Volume and Issue: 15(20), P. 2011 - 2027

Published: Jan. 1, 2024

In this review, various strategies of enzyme-catalysed RAFT polymerization are discussed for the synthesis sustainable and precision polymers via green polymer chemistry.

Language: Английский

Citations

4
Bridging the Gap: Polymer Materials with Controlled Architecture and Precise Functionalization to Overcome Biofouling Challenges for Biosensing, Drug Delivery, and Bioseparation DOI
Andrew Trowbridge,

Nigel T. Bennett,

Thomas M. Reimer

et al.

Polymer, Journal Year: 2024, Volume and Issue: 307, P. 127230 - 127230

Published: June 3, 2024

Language: Английский

Citations

4
Nanozymes with Modulable Inhibition Transfer Pathways for Thiol and Cell Identification DOI
Lijun Hu, Lei Jiao, Chengjie Chen

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

The elementary mechanism and site studies of nanozyme-based inhibition reactions are ambiguous urgently require advanced nanozymes as mediators to elucidate the effect. To this end, we develop a class featuring single Cu–N catalytic configurations B–O sites binding on porous nitrogen-doped carbon substrate (B6/CuSA) for inducing modulable transfer at atomic level. full redistribution electrons across sites, induced by incorporation, yields B6/CuSA with enhanced peroxidase-like activity versus CuSA. More importantly, CuSA features in cysteine expresses competitive through coordination bonds, an constant 0.048 mM. Benefiting from way nanozymes, possesses mixed approaches noncovalent bonds delivers record-mixed interaction 0.054 mM noncompetitive 0.71 Based CuSA, multichannel sensor array accomplishes detection various cancer cells, normal thiols. design principle work is endowed guidelines preliminary evaluation massive potential thiols, cell discrimination, disease prediction.

Language: Английский

Citations

0
Synthesis of Star Polymers with Ultrahigh Molecular Weights and Tunable Dispersities via Photoiniferter Polymerization DOI
Mei Zhang, Steven P. Armes, Zesheng An

et al.

ACS Macro Letters, Journal Year: 2025, Volume and Issue: unknown, P. 306 - 312

Published: Feb. 21, 2025

Simultaneous control over macromolecular chain topology, molecular weight, and dispersity is an important synthetic goal in polymer chemistry. The synthesis of well-defined poly(methyl acrylate) star polymers with ultrahigh weights (>106 g mol-1) tunable dispersities realized for the first time via blue light-controlled photoiniferter polymerization using a tetrafunctional switchable RAFT agent (SRA4). spectroscopic properties activity SRA4 can be reversibly tuned by addition acid/base. For example, protonation 4-toluenesulfonic acid (TsOH) leads to enhanced UV-visible light absorption, faster rate, lower resulting polymer. Star were prepared predicted (Mn ≈ 80-1550 kg (Đ 1.8-1.2) when targeting degrees range 1000-20000 presence varying amounts TsOH. High end-group fidelity such was confirmed one-pot extension experiments, which afforded series pseudoblock copolymers controlled dispersities. Finally, rotational rheology used examine effect dispersity, topology (whether linear or star-shaped) on solution viscosity.

Language: Английский

Citations

0
Enzyme‐Catalyzed Controlled Radical Polymerization DOI Open Access
Ruoyu Li, Zesheng An

Published: March 7, 2025

Language: Английский

Citations

0
Self-decorating cells via surface-initiated enzymatic controlled radical polymerization DOI Creative Commons
Andrea Belluati, Dominic Happel,

Malte Erbe

et al.

Nanoscale, Journal Year: 2023, Volume and Issue: 15(48), P. 19486 - 19492

Published: Jan. 1, 2023

Through the innovative use of surface-displayed horseradish peroxidase, this work explores enzymatic catalysis both bioRAFT polymerization and bioATRP to prompt polymer synthesis on surface Saccharomyces cerevisiae cells, with outperforming polymerization. The resulting modification living yeast cells synthetic polymers allows for a significant change in phenotype, including growth profile, aggregation characteristics, conjugation non-native enzymes clickable cell surface, opening new avenues bioorthogonal cell-surface engineering.

Language: Английский

Citations

10
Coacervate Nanoreactors: PEG Side-Chain-Assisted Compartmentalization Leads to an Oxygen-Tolerant Polymerization-Induced Electrostatic Self-Assembly DOI
Xiyu Wang, Qingbin Zheng, Xinhua Lu

et al.

Macromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Language: Английский

Citations

0
Bacteria-Mediated Intracellular Radical Polymerizations DOI Creative Commons

Eleonora Ornati,

Jules Perrard,

T. Hoffmann

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Intracellular radical polymerizations allow for the direct bioorthogonal synthesis of various synthetic polymers within living cells, thereby providing a pathway to polymer-modified cells or fermentative production polymers. Here, we show that Escherichia coli can initiate polymerization acrylamide, acrylic, and methacrylic monomers through an atom transfer reaction triggered by activity naturally occurring biomolecules bacterial cells. were confirmed nuclear magnetic resonance spectroscopy, gel permeation chromatography extracted from fluorescence labeling polymer directly inside The effect on cell behavior response was investigated microscopy flow cytometry techniques, as well metabolic membrane integrity assays. resulting products are cell-compatible, indicated high viability polymerized In cellulo containing fluorescent dyes also achieved. These results not only enhance our understanding untapped potential catalysts but reveal intracellular based initiators tool engineering biology.

Language: Английский

Citations

0