A Single Multiomics Transistor for Electronic Detection of SARS‐Cov2 Variants Antigen and Viral RNA Without Amplification DOI Creative Commons
Deependra Kumar Ban, Reza Hajian, Alexander Winton

et al.

Advanced Materials Technologies, Journal Year: 2023, Volume and Issue: 8(11)

Published: April 4, 2023

Abstract The SARS‐CoV‐2 pandemic caused a public health crisis throughout the world and highlighted need for rapid sensitive testing as countermeasure. A specific biosensor platform is developed detection of antigen RNA SARS‐CoV‐2, its variant (B1.1.529). demonstrated combines unique protein catalyzed capture bioreceptors (PCCs) chimeric (RNA‐DNA) probe using LwaCas13a collateral cleavage activity atop graphene field effect transistors (gFETs). reported able to differentiate unprocessed 10 4 pfu m −1 samples from Influenza Rhinovirus. limit (LOD) calculated 3 in buffer PFU mL 10% saliva, while LOD ≈65 viral isolate without amplification. To provide high reliability detection, role internal external factors with respect gate voltage further analyzed by Principal Component Analysis (PCA). Based on PCA analysis, authors are classify pathogen positive or negative ( Y > 0: Positive pathogen, < Negative pathogen). can be quickly adapted multi‐omics multiplexed diagnosis continuously evolving biothreats global pandemics.

Language: Английский

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives DOI
Huimin Li, Yi Xie, Fumin Chen

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 52(1), P. 361 - 382

Published: Dec. 19, 2022

This review summarizes the recent advances and main strategies to improve sensitivity of amplification-free CRISPR/Cas-based detection techniques.

Language: Английский

Citations

164
CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics DOI Open Access

Zhengyan Weng,

Zheng You, Jie Yang

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(17)

Published: Jan. 30, 2023

Abstract Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires long turnaround time, expensive equipment, trained personnel, limiting its widespread availability diagnostic capacity. On the other hand, clustered regularly interspaced short palindromic repeats (CRISPR) technology recently demonstrated capability for detection with high sensitivity specificity. CRISPR‐mediated biosensing holds great promise revolutionizing procedures developing point‐of‐care diagnostics. This review focuses on recent developments both fundamental CRISPR biochemistry CRISPR‐based techniques. Four ongoing research hotspots molecular diagnostics‐target preamplification‐free detection, microRNA (miRNA) testing, non‐nucleic‐acid SARS‐CoV‐2 detection‐are also covered.

Language: Английский

Citations

106
Amplification‐Free Detection of SARS‐CoV‐2 and Respiratory Syncytial Virus Using CRISPR Cas13a and Graphene Field‐Effect Transistors DOI
Huijie Li, Jie Yang, Guangfu Wu

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(32)

Published: May 12, 2022

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR-based biosensors infectious respiratory disease diagnostic still require target preamplifications. This study reports a new biosensor amplification-free detection via harnessing trans-cleavage mechanism Cas13a and ultrasensitive graphene field-effect transistors (gFETs). CRISPR Cas13a-gFET achieves SARS-CoV-2 syncytial virus (RSV) genome down to 1 attomolar without Additionally, we validate performance using clinical samples, including those with low viral loads (Ct value >30). Overall, these findings establish our among most sensitive platforms date.

Language: Английский

Citations

71
A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel DOI Open Access
Long Ma, Wenlu Zhang, Lijuan Yin

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 452, P. 131195 - 131195

Published: March 11, 2023

Language: Английский

Citations

61
Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device DOI Creative Commons
Yunxiang Wang, Hong Chen, Kai Lin

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 16, 2024

Abstract The emerging monkeypox virus (MPXV) has raised global health concern, thereby highlighting the need for rapid, sensitive, and easy-to-use diagnostics. Here, we develop a single-step CRISPR-based diagnostic platform, termed SCOPE (Streamlined CRISPR On Pod Evaluation platform), field-deployable ultrasensitive detection of MPXV in resource-limited settings. viral nucleic acids are rapidly released from rash fluid swab, oral saliva, urine samples 2 min via streamlined lysis protocol, followed by 10-min recombinase polymerase amplification (RPA)-CRISPR/Cas13a reaction. A pod-shaped vest-pocket analysis device achieves whole process reaction execution, signal acquisition, result interpretation. can detect as low 0.5 copies/µL (2.5 copies/reaction) within 15 sample input to answer. We validate developed assay on 102 clinical male patients / volunteers, testing results 100% concordant with real-time PCR. single-molecular level sensitivity minutes simplified procedure performed miniaturized wireless device, which is expected spur substantial progress enable practice application diagnostics techniques point-of-care setting.

Language: Английский

Citations

35
Polyoxometalates: Cascading Functionality to Unique On‐Off Organic Photoelectrochemical Transistor Operation DOI
Feng‐Zao Chen,

Lu Hou,

Yuan Gao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(48)

Published: June 29, 2024

Abstract While existing polyoxometalates (POMs) are predominantly utilized in a range of energy devices, their potential bioelectronics and optoelectronics, particularly organic photoelectrochemical transistors (OPECTs), remains unexplored. Here, the POMs this aspect is unveiled by POM/sulfide‐gated OPECT. The representative PW 12 /ZnIn 2 S 4 with cascading light‐harvesting enzyme‐like functionality enables unique on‐off OPECT operation. incorporation facilitates separation photogenerated charge carriers across heterojunction interface, consequently inducing pronounced gating effect. Linking CRISPR/Cas13a system correlating target miRNA‐21 biological generation H O , will enable polymerization dopamine into polydopamine thus sensitively inhibit gating. Experimental results demonstrated that developed sensor achieved good analytical performance detection limit as low 3.9 f m . This study demonstrates Given large family multifunction POMs, its implications diverse optoelectronics expected.

Language: Английский

Citations

30
Recent Advances in Graphene Field‐Effect Transistor Toward Biological Detection DOI
Mingyuan Sun, Congcong Zhang, Shan Lu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(44)

Published: May 22, 2024

Abstract Recently, field‐effect transistors (FETs) have emerged as a novel type of multiparameter, high‐performance, highly integrated platform for biochemical detection, leveraging their classical three‐terminal structure, working principles, and fabrication methods. Notably, graphene materials, known exceptional electrical optical properties well biocompatibility, serve fundamental component these devices, further enhancing advantages in biological detection. This review places special emphasis on recent advancements transistor (GFET)‐based biosensors focuses four main areas: i) the basic concepts FETs specific GFETs; ii) various state‐of‐the‐art approaches to enhance performance GFET‐based terms operating principles “3S”—stability, sensitivity, specificity; iii) multiplexed detection strategies biosensors; iv) current challenges future perspectives field biosensors. It is hoped that this article can profoundly elucidate development GFET inspire broader audience.

Language: Английский

Citations

20
Challenges for Field-Effect-Transistor-Based Graphene Biosensors DOI Open Access
Takao Ono, Satoshi Okuda, Shota Ushiba

et al.

Materials, Journal Year: 2024, Volume and Issue: 17(2), P. 333 - 333

Published: Jan. 9, 2024

Owing to its outstanding physical properties, graphene has attracted attention as a promising biosensor material. Field-effect-transistor (FET)-based biosensors are particularly because of their high sensitivity that is achieved through the carrier mobility graphene. However, graphene-FET have not yet reached widespread practical applications owing several problems. In this review, authors focus on and discuss advantages, challenges development, solutions challenges. The problem Debye screening, in which surface charges detection target shielded undetectable, can be solved by using small-molecule receptors deformations enzyme reaction products. To address complexity sample components mechanisms biosensors, outline measures against nonspecific adsorption remaining problems related mechanism itself. also introduce solution with molecular species reach sensor surfaces limited. Finally, present multifaceted approaches provide much information corroborate results electrical measurements. introduced bring us closer realization stable utilizing superior characteristics

Language: Английский

Citations

19
Amplification-Free Detection of Mycobacterium Tuberculosis Using CRISPR-Cas12a and Graphene Field-Effect Transistors DOI
Weiqi Wang, Hongqiang Du, Changhao Dai

et al.

Nanoscale, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Current molecular tests for tuberculosis (TB), such as whole genome sequencing and Xpert Mycobacterium tuberculosis/rifampicin resistance assay, exhibit limited sensitivity necessitate the pre-amplification step of target DNA. This limitation greatly increases detection time poses an increased risk infection. Here, we present a graphene field-effect transistor (GFET) based on CRISPR/Cas system detecting tuberculosis. The CRISPR/Cas12a has ability to specifically recognize cleave By integrating onto FET platform utilizing its electrical amplification capability, achieve rapid sensitive without requiring sample pre-amplification, with limit (LoD) low 2.42 × 10-18 M. Cas12a-GFET devices can differentiate 30 positive cases from 56 serum samples within 5 minutes. These findings highlight immense potential in future biological analysis clinical diagnosis.

Language: Английский

Citations

2
CRISPR-Cas13a system: A novel tool for molecular diagnostics DOI Creative Commons

Lixin Zhao,

Minyue Qiu,

Xiaojia Li

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 8, 2022

The clustered regularly interspaced short palindromic repeats (CRISPR) system is a natural adaptive immune of prokaryotes. CRISPR-Cas currently divided into two classes and six types: types I, III, IV in class 1 systems II, V, VI 2 systems. Among the type systems, CRISPR/Cas13a has been most widely characterized for its application molecular diagnostics, gene therapy, editing, RNA imaging. Moreover, because trans-cleavage activity Cas13a high specificity CRISPR RNA, enormous potential field diagnostics. Herein, we summarize applications detection pathogens, including viruses, bacteria, parasites, chlamydia, fungus; biomarkers, such as microRNAs, lncRNAs, circRNAs; some non-nucleic acid targets, proteins, ions, methyl groups. Meanwhile, highlight working principles novel Cas13a-based methods, Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK) improved versions, nucleic amplification-free biosensors, biosensors target detection. Finally, focus on issues that need to be solved development prospects system.

Language: Английский

Citations

49