A hydrophobic photouncaging reaction to profile the lipid droplet interactome in tissues DOI Creative Commons
Di Shen, Qun Zhao,

Huaiyue Zhang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(16)

Published: April 16, 2025

Most bioorthogonal photouncaging reactions preferentially occur in polar environments to accommodate biological applications the aqueous cellular milieu. However, they are not precisely designed chemically adapt diverse microenvironments of cell. Herein, we report a hydrophobic reaction with tailored photolytic kinetics toward solvent polarity. Structural modulations aminobenzoquinone-based photocage reveal impact cyclic ring size, steric substituent, and electronic substituent on individual uncaging ( k H2O dioxane ) polarity preference / ). Rational incorporation optimized moieties leads up 20.2-fold nonpolar kinetic selectivity Further photochemical spectroscopic characterizations theoretical calculations together uncover mechanism underlying polarity-dependent kinetics. The uncaged ortho-quinone methide product bears covalent reactivity nucleophiles protein revealed by tandem mass spectrometry. Finally, demonstrate application such lipophilic chemistry selective labeling profiling proteins proximity lipid droplets inside human fatty liver tissues. Together, this work studies effects adapts it suborganelle-targeted profiling.

Language: Английский

Rejuvenating old fluorophores with new chemistry DOI
Martin J. Schnermann, Luke D. Lavis

Current Opinion in Chemical Biology, Journal Year: 2023, Volume and Issue: 75, P. 102335 - 102335

Published: June 1, 2023

Language: Английский

Citations

18
Red/near-infrared light triggered photorelease via sensitized photolysis DOI
Donghao Lu,

Shu Yang,

Qiyu Yu

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 518, P. 216117 - 216117

Published: July 27, 2024

Language: Английский

Citations

6
Light‐Activated Anti‐Vascular Combination Therapy against Choroidal Neovascularization DOI Creative Commons
Shuting Xu, Jia Li, Kaiqi Long

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(40)

Published: Aug. 29, 2024

Abstract Choroidal neovascularization (CNV) underlies the crux of many angiogenic eye disorders. Although medications that target vascular endothelial growth factor (VEGF) are approved for treating CNV, their effectiveness in destroying new blood vessels is limited, and invasive intravitreal administration required. Additionally, other drugs destroy established neovessels, such as combretastatin A‐4, may have systemic side effects limit therapeutic benefits. To overcome these shortcomings, a two‐pronged anti‐vascular approach presented CNV treatment using photoactivatable nanoparticle system can release VEGF receptor inhibitor disrupting agent when irradiated with 690 nm light. The nanoparticles be injected intravenously to enable anti‐angiogenic combination therapy through light irradiation eyes. This potentiate while maintaining favorable biosafety profile choroidal diseases.

Language: Английский

Citations

5
Dimerized Pentamethine Cyanines for Synergistically Boosting Photodynamic/Photothermal Therapy DOI Creative Commons

Haiqiao Huang,

Qiang Liu, Jinghui Zhu

et al.

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

ABSTRACT Photodynamic therapy (PDT) and photothermal (PTT) have emerged promising applications in both fundamental research clinical trials. However, it remains challenging to develop ideal photosensitizers (PSs) that concurrently integrate high photostability, large near‐infrared absorptivity, efficient therapeutic capabilities. Herein, we reported a sample engineering strategy afford benzene‐fused Cy5 dimer ( Cy‐D‐5 ) for synergistically boosting PDT/PTT applications. Intriguingly, exhibits tendency form J ‐aggregates H phosphate‐buffered saline, which show long‐wavelength absorption band bathochromically shifted 810 nm short‐wavelength hypsochromically 745 nm, respectively, when compared its behavior ethanol (778 nm). Density‐functional theory calculations combined with time‐resolved transient optical spectroscopy analysis reveal the fused low Δ E ST (0.51 eV) non‐radiative transition rates (12.6 times greater than of clinically approved PS‐indocyanine green [PS‐ ICG ]). Furthermore, demonstrates higher photosensitizing ability produce 1 O 2 , stronger conversion efficiency η = 64.4%), photostability . These properties enable achieve complete tumor ablation upon 808 laser irradiation, highlighting potential as powerful dual‐function phototherapeutic agent. This work may offer practical other existing dyes red‐shifted spectral range various phototherapy

Language: Английский

Citations

5
Deuteration of heptamethine cyanine dyes enhances their emission efficacy DOI Creative Commons
Hana Janeková, Hannah C. Friedman, Marina Russo

et al.

Chemical Communications, Journal Year: 2023, Volume and Issue: 60(8), P. 1000 - 1003

Published: Dec. 18, 2023

The design of bright short-wave infrared fluorophores remains a grand challenge. Here we investigate the impact deuteration on properties in series heptamethine dyes, absorption which spans near-infrared and SWIR regions. We demonstrate that it is generally applicable strategy leads to enhanced quantum yields fluorescence, longer-lived singlet excited states suppressed rates non-radiative deactivation processes.

Language: Английский

Citations

13
The emerging tools for precisely manipulating microtubules DOI Creative Commons

Yen-Ling Lian,

Yu‐Chun Lin

Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 88, P. 102360 - 102360

Published: April 18, 2024

Cells generate a highly diverse microtubule network to carry out different activities. This is comprised of distinct tubulin isotypes, tubulins with post-translational modifications, and many microtubule-based structures. Defects in this complex system cause numerous human disorders. However, how subtypes regulate cellular architectures activities remains largely unexplored. Emerging tools such as photosensitive pharmaceuticals, chemogenetics, optogenetics enable the spatiotemporal manipulation structures, dynamics, cross-linking actin filaments target subtypes. review summarizes design rationale applications these new approaches aims provide roadmap for researchers navigating intricacies dynamics their modifications contexts, thereby opening avenues therapeutic interventions.

Language: Английский

Citations

4
Concurrent Subcellular Delivery of Hydrogen Sulfide and a Payload with Near-Infrared Light DOI Creative Commons

Katarzyna Hanc,

Hana Janeková, Peter Štacko

et al.

JACS Au, Journal Year: 2024, Volume and Issue: 4(7), P. 2687 - 2694

Published: July 5, 2024

Hydrogen sulfide (H2S) is a gaseous signaling molecule, exerting crucial regulatory functions in organelles and cellular environments. H2S exhibits high therapeutic potential synergistic effects with other drugs, its potency notably enhanced through organelle-specific targeting. Yet, the navigation of light-activated donors to specific remains absent. Here, we report first photocage that simultaneously delivers payload subcellular precision mitochondria live human cells using tissue-penetrating near-infrared light as trigger. The fluorogenic enables real-time monitoring process, demonstrate concurrent uncaging combination fluorescence microscopy mitochondria-specific fluorescent probes. We anticipate these photocages will permit precise delivery H2S-drug combinations exceptional spatiotemporal control, thereby driving harnessing known discovery novel strategies.

Language: Английский

Citations

4
Thioketal-photocage: a universal modification strategy for constructing new photochemical tools for real-time imaging in living cells DOI

Ding‐Heng Zhou,

Shanyong Chen, Yan-Zhao Liu

et al.

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Spatio-temporal control of mitosis using light via a Plk1 inhibitor caged for activity and cellular permeability DOI Creative Commons
Victoria von Glasenapp, Ana C. Almeida, Dalu Chang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

Abstract The ability to control the activity of kinases spatially and temporally is essential elucidate role signalling pathways in development physiology. Progress this direction has been hampered by lack tools manipulate kinase a highly controlled manner vivo. Here we report strategy modify BI2536, well characterized inhibitor conserved mitotic Polo-like 1 (Plk1). We introduce same coumarin photolabile protecting group (PPG) at two positions inhibitor. At one position, prevents interaction with Plk1, second it masks an added carboxylic acid, important for cellular retention. Exposure light results removal both PPGs, leading activation its trapping inside cells. demonstrate efficacy caged three-dimensional spheroid cultures: uncaging single pulse, can inhibit Plk1 arrest cell division, dynamic process, spatio-temporal control. Our design be applied other small molecules, providing solution their living cells unprecedented precision.

Language: Английский

Citations

0
Conceptual expansion of photomedicine for spatiotemporal treatment methods DOI Creative Commons
P. K. Hashim,

Ashwin T Shaji,

Ammathnadu S. Amrutha

et al.

RSC Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review covers the history of light therapy, emergence photopharmacology, its methods, and challenges. Current advancements show promise for treating diseases like cancer with precision minimal side effects.

Language: Английский

Citations

0