Nanoencapsulation of Living Microbial Cells in Porous Covalent Organic Framework Shells DOI

Chen Li,

Mengchu Feng,

Bixiao Li

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Encapsulating living cells within nanoshells offers an important approach to enhance their stability against environmental stressors and broaden application scope. However, this often leads impaired mass transfer at the cell biointerface. Strengthening protective shell with well-defined, ordered transport channels is crucial regulating molecular maintaining viability biofunctionality. Herein, we report construction of covalent organic framework (COF) mesoporous shells for single-cell nanoencapsulation, providing selective permeability comprehensive protection microbial cells. The COF ensure nutrient uptake while blocking large harmful molecules UV-C radiation, thereby preserving metabolic activity. Integration such crystalline porous genetically modified factories production further investigated, revealing no adverse effects, as demonstrated by riboflavin production. Moreover, effectively shields cells, ensuring efficient bioproduction even after being treated under harsh conditions. This versatile encapsulation applicable different types, a robust platform surface engineering.

Language: Английский

Covalent organic frameworks in heterogeneous catalysis: recent advances and future perspective DOI
Ziad Alsudairy,

Normanda Brown,

Allea Campbell

et al.

Materials Chemistry Frontiers, Journal Year: 2023, Volume and Issue: 7(16), P. 3298 - 3331

Published: Jan. 1, 2023

This review provides an overview of the recent advances in heterogeneous catalysis using COFs, primarily from 2020 to 2023, covering fundamentals, advantages, current challenges, and future perspectives.

Language: Английский

Citations

68
Encapsulating and stabilizing enzymes using hydrogen-bonded organic frameworks DOI
Guosheng Chen, Siming Huang, Xiaomin Ma

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(7), P. 2032 - 2050

Published: May 17, 2023

Language: Английский

Citations

62
Harnessing Self-Repairing and Crystallization Processes for Effective Enzyme Encapsulation in Covalent Organic Frameworks DOI
Yufeng Zhang,

Chunyan Xing,

Zhenjie Mu

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(24), P. 13469 - 13475

Published: June 7, 2023

Immobilization of fragile enzymes in crystalline porous materials offers new opportunities to expand the applications biocatalysts. However, limited by pore size and/or harsh synthesis conditions hosts, often suffer from dimension limitation or denaturation during immobilization process. Taking advantage dynamic covalent chemistry feature organic frameworks (COFs), herein, we report a preprotection strategy encapsulate COFs self-repairing and crystallization Enzymes were first loaded low-crystalline polymer networks with mesopores formed at initial growth stage, which could offer effective protection for reaction conditions, subsequently encapsulation proceeded disordered into framework. Impressively, biological activity can be well-maintained after encapsulation, obtained enzyme@COFs also show superior stability. Furthermore, circumvents enzymes, its versatility was verified different sizes surface charges, as well two-enzyme cascade system. This study universal design idea robust supports holds promise developing high-performance immobilized

Language: Английский

Citations

58
Enzyme immobilization on covalent organic framework supports DOI
Qianqian Zhu,

Yunlong Zheng,

Zhenjie Zhang

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(10), P. 3080 - 3125

Published: Sept. 6, 2023

Language: Английский

Citations

52
Insight into Bioactivity of In‐situ Trapped Enzyme‐Covalent‐Organic Frameworks DOI Creative Commons
Jieying Liang, Juanfang Ruan,

Bosiljka Njegic

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(23)

Published: April 6, 2023

Abstract Selecting a suitable support material for enzyme immobilization with excellent biocatalytic activity and stability is critical aspect in the development of functional biosystems. The highly stable metal‐free properties covalent‐organic frameworks (COFs) make them ideal supports immobilization. Herein, we constructed three kinds COFs via biofriendly one‐pot synthetic strategy at room temperature aqueous solution. Among developed (COF‐LZU1, RT‐COF‐1 ACOF‐1), horseradish peroxidase (HRP)‐incorporated COF‐LZU1 found to retain highest activity. Structural analysis reveals that weakest interaction between hydrated COF‐LZU1, an easiest accessibility by substrate, as well optimal conformation together promote bioactivity HRP‐COF‐LZU1. Furthermore, revealed be versatile nanoplatform encapsulating multiple enzymes. also offers superior protection immobilized enzymes under harsh conditions during recycling. comprehensive understanding interfacial interactions COF host guest, substrate diffusion, alteration within matrices represents opportunity design biocatalysts opens broad range applications these nanosystems.

Language: Английский

Citations

50
Green synthesis of stable hybrid biocatalyst using a hydrogen-bonded, π-π-stacking supramolecular assembly for electrochemical immunosensor DOI Creative Commons
Wei Huang, Haitao Yuan, Hongling Yang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: June 20, 2023

Abstract Rational integration of native enzymes and nanoscaffold is an efficient means to access robust biocatalyst, yet remains on-going challenges due the trade-off between fragile harsh assembling conditions. Here, we report a supramolecular strategy enabling in situ fusion into porous crystal. A c2-symmetric pyrene tecton with four formic acid arms utilized as building block engineer this hybrid biocatalyst. The decorated afford tectons high dispersibility minute amount organic solvent, permit hydrogen-bonded linkage discrete extended network around enzyme almost solvent-free aqueous solution. This biocatalyst covered by long-range ordered pore channels, which can serve gating sieve catalytic substrate thus enhance biocatalytic selectivity. Given structural integration, biocatalyst-based electrochemical immunosensor developed, pg/mL detection cancer biomarker.

Language: Английский

Citations

50
Construction of catalase@hollow silica nanosphere: Catalase with immobilized but not rigid state for improving catalytic performances DOI
Yingjie Du,

Lixue Zhao,

Zixin Geng

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130381 - 130381

Published: Feb. 21, 2024

Language: Английский

Citations

19
Post‐Nickelation of a Crystalline Trinuclear Copper Organic Framework for Synergistic Photocatalytic Carbon Dioxide Conversion DOI

Xinxin Wang,

Xu Ding,

Yucheng Jin

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(18)

Published: March 9, 2023

Rational regulation of electronic structures and functionalities framework materials still remains challenging. Herein, reaction 4,4',4''-nitrilo-tribenzhydrazide with tris(μ2 -4-carboxaldehyde-pyrazolato-N,N')-tricopper (Cu3 Py3 ) generates the crystalline copper organic USTB-11(Cu). Post-modification divalent nickel ions affords heterometallic USTB-11(Cu,Ni). Powder X-ray diffraction theoretical simulations reveal their two-dimensional hexagonal structure geometry. A series advanced spectroscopic techniques disclose mixed CuI /CuII state nature Cu3 in USTB-11(Cu,Ni) a uniform bistable Cu34+ (CuI2 CuII : Cu35+ (CuI CuII2 (ca. 1 3) oxidation state, resulting significantly improved formation efficiency charge-separation state. This endows Ni sites enhanced activity outstanding photocatalytic CO2 to CO performance conversion rate 22 130 μmol g-1 h-1 selectivity 98 %.

Language: Английский

Citations

40
Review of covalent organic frameworks for enzyme immobilization: Strategies, applications, and prospects DOI
Jie Feng, Qingyun Huang, Ce Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 248, P. 125729 - 125729

Published: July 7, 2023

Language: Английский

Citations

39
Nanoplatforms Based on Covalent Organic Frameworks (COFs) for Biomedical Applications DOI
Shuang Liang, Menghao Li, Manlin Qi

et al.

Chemistry of Materials, Journal Year: 2023, Volume and Issue: 35(20), P. 8353 - 8370

Published: Oct. 12, 2023

Covalent organic frameworks (COFs) make up an emerging class of crystalline porous materials mainly composed light elements in the form dynamic covalent bonds. Owing to their two- or three-dimensional network structures and ideal properties, including low density, large specific surface area, high chemical stability, good biocompatibility, COFs have shown a wide range applications optoelectronic devices, energy conversion storage, adsorption separation, sensing, catalysis, biomedicine. This review provides overview recent advances functional COF-based nanoplatforms for biological diagnosis treatment, such as enzyme protein drug delivery, photodynamic/photothermal therapy, synergistic treatment. Challenges future directions developing biomedicine related are also discussed. We envisage that this will inspire scientists, chemists, biologists, clinical doctors working fields work closely move field toward trials human healthcare.

Language: Английский

Citations

39