Critical review and recent advances of emerging real-time and non-destructive strategies for meat spoilage monitoring

Food Chemistry, Journal Year: 2024, Volume and Issue: 445, P. 138755 - 138755

Published: Feb. 15, 2024

Language: Английский

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5-Hydroxymethylcytosine modifications in circulating cell-free DNA: frontiers of cancer detection, monitoring, and prognostic evaluation

Biomarker Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: March 7, 2025

Abstract Developing accurate, clinically convenient, and non-invasive methods for early cancer detection, monitoring, prognosis assessment is essential improving patient survival rates, enhancing quality of life, reducing the socioeconomic burden associated with cancer. This goal critical in precision oncology. Genetic epigenetic alterations circulating cell-free DNA (cfDNA) have emerged as transformative tools advancing outcomes. Among these, 5-hydroxymethylcytosine (5hmC) modifications cfDNA stand out promising markers, offering insights into initiation, progression, metastasis, across various types, such lung cancer, colorectal hepatocellular carcinoma. review comprehensively explores biology sequencing methodologies 5hmC, emphasizing their potential screening, diagnosis, treatment prognostic assessment. It highlights recent advancements cfDNA-derived 5hmC signatures’ applications, addressing strengths limitations context clinical translation. Furthermore, this outlines key challenges future directions integrating routine practice, facilitating personalized management.

Language: Английский

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Mapping epigenetic modifications by sequencing technologies

Cell Death and Differentiation, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 1, 2023

The "epigenetics" concept was first described in 1942. Thus far, chemical modifications on histones, DNA, and RNA have emerged as three important building blocks of epigenetic modifications. Many been intensively studied found to be involved most essential biological processes well human diseases, including cancer. Precisely quantitatively mapping over 100 [1], 17 [2], 160 [3] different known types histone, is the key understanding role gene regulation diverse processes. With rapid development sequencing technologies, scientists are able detect specific with various quantitative, high-resolution, whole-genome/transcriptome approaches. Here, we summarize recent advances modification focusing major mammalian cells.

Language: Английский

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Targeting the p53 signaling pathway in cancers: Molecular mechanisms and clinical studies

MedComm, Journal Year: 2023, Volume and Issue: 4(3)

Published: May 28, 2023

Tumor suppressor p53 can transcriptionally activate downstream genes in response to stress, and then regulate the cell cycle, DNA repair, metabolism, angiogenesis, apoptosis, other biological responses. has seven functional domains 12 splice isoforms, different subtypes play roles. The activation inactivation of are finely regulated associated with phosphorylation/acetylation modification ubiquitination modification, respectively. Abnormal is closely related occurrence development cancer. While targeted therapy signaling pathway still its early stages only a few drugs or treatments have entered clinical trials, new ongoing trials expected lead widespread use signaling-targeted cancer treatment future. TRIAP1 novel inhibitor apoptosis. homolog yeast mitochondrial intermembrane protein MDM35, which tumor-promoting role by blocking mitochondria-dependent apoptosis pathway. This work provides systematic overview recent basic research progress proposes that an important therapeutic target signaling.

Language: Английский

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Mechanisms and Advances of Epigenetic Regulation in Cardiovascular Disease

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(6)

Published: May 28, 2024

Epigenetics refers to heritable changes in gene expression and function that impact nuclear processes associated with chromatin, all without altering DNA sequences. These epigenetic patterns, being traits, are vital biological mechanisms intricately regulate heredity. The application of chemical labeling single-cell resolution mapping strategies has significantly facilitated large-scale modifications nucleic acids over recent years. Notably, can induce phenotypic changes, cell differentiation, influence cell-specific expression, parentally imprint genes, activate the X chromosome, stabilize genome structure. Given their reversibility susceptibility environmental factors, have gained prominence disease diagnosis, impacting clinical medicine research. Recent studies uncovered strong links between pathogenesis metabolic cardiovascular diseases, including congenital heart disease, failure, cardiomyopathy, hypertension, atherosclerosis. In this review, we provide an overview progress research within context encompassing pathogenesis, prevention, treatment. Furthermore, shed light on potential prospects acid as a promising avenue biomedical applications.

Language: Английский

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Understanding the role of DNA methylation in colorectal cancer: Mechanisms, detection, and clinical significance

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2024, Volume and Issue: 1879(3), P. 189096 - 189096

Published: March 17, 2024

Language: Английский

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Synthesis and Resolution of 4′-Substituted Nucleosides with Potential Antiviral and Antisense Strategies

The Journal of Organic Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Nucleoside derivatives having a 4'-substituent show promise as potential antiviral agents well nucleoside units for constructing nucleic acid medicines. To develop new nucleosides, it is crucial to achieve feasible access the intended derivatives, encompassing both enantiomers. Toward this end, we started synthesizing an achiral 4-hydroxymethyldihydrofuran sugar precursor, which subjected oxidative glycosylation reaction using hypervalent iodine. The resulting racemate of 4'-hydroxymethylated thymidine derivative underwent kinetic resolution lipase, yielding d- and l-isomers with high optical purity. d-4'-hydroxymethylstavudine was then converted into corresponding phosphoramidite derivative, from oligonucleotide synthesized.

Language: Английский

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Comparison of research trends and hotspots of DNA and RNA methylation in asthma-related studies based on bibliometric analysis

Deleted Journal, Journal Year: 2025, Volume and Issue: 7(3)

Published: Feb. 22, 2025

Language: Английский

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Advances and Challenges in Solid-State Nanopores for DNA Sequencing

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Solid-state nanopore sensing, a state-of-the-art technology for single-molecule detection, has rapidly advanced in recent years and demonstrates significant potential DNA sequencing. This determines the nucleotide sequences by analyzing electrical or optical signal variations that occur when molecules pass through nanopore. It offers notable advantages, including high-throughput, real-time monitoring, elimination of need polymerase chain reaction (PCR) amplification, thereby presenting broad application prospects areas such as diagnosis treatment genetic diseases. paper reviews solid-state sequencing discussing advancements types, preparation techniques, detection methods. examines various materials, silicon-based materials two-dimensional (2D) well techniques transmission electron microscopy (TEM), focused ion beam (FIB) etching, controlled breakdown (CBD). Additionally, it elucidates mechanisms, ion-current blockade, transverse-current detection. However, this faces numerous challenges its implementation future commercialization. For instance, limited spatial resolution hampers single-base identification; rapid translocation speed impacts time resolution; types noise significantly disrupt signals. In response, researchers have proposed several solutions, local thinning film, adjustment surface charges, optimization structures. With interdisciplinary integration technological innovation, is expected to make breakthroughs, bringing transformations life sciences research medical diagnosis.

Language: Английский

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Embryonic Zebrafish as a Model for Investigating the Interaction between Environmental Pollutants and Neurodegenerative Disorders

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1559 - 1559

Published: July 13, 2024

Environmental pollutants have been linked to neurotoxicity and are proposed contribute neurodegenerative disorders. The zebrafish model provides a high-throughput platform for large-scale chemical screening toxicity assessment is widely accepted as an important animal the investigation of Although recent studies explore roles environmental in disorders models, current knowledge mechanisms environmentally induced relatively complex overlapping. This review primarily discusses utilizing embryonic investigate pollutants-related disease. We also applicable approaches biomarkers unravel underlying mechanism related found be powerful tool that evaluating triggered by relevant concentrations neurotoxic compounds. Additionally, using variable assess allows researchers insights into interaction between and, ultimately, understanding toxicants.

Language: Английский

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