Textural Precursor Compositions Harvested for Independent Signal Generators: Scaling Micron-Sized Flower-Like Metal–Organic Frameworks as Amplifying Units for Dual-Mode Glycoprotein Assay

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(23), P. 9503 - 9511

Published: May 23, 2024

In this work, a micron-sized flower-like metal–organic frameworks (MOFs)-based boronate-affinity sandwich-type immunoassay was fabricated for the dual-mode glycoprotein assay. For proof of concept, MOFs were synthesized from transition Cu nodes and tetrakis (4-carboxyphenyl) porphyrin (TCPP) ligands by spontaneous standing assembly. addition, specificity toward involved antigen recognition as well covalent bonding via boronate-glycan affinity, immediate signal responses initiated textural decomposition MOFs. Intriguingly, nodes, which valence state is dominant CuI species, can endow Fenton-like catalytic reaction fluorogenic substrate generating fluorescence signals. benefits, TCPP ligands, in each molecule has four guest donors, provide multiple valences assembly cyclodextrin-capped gold nanoparticles host–guest interaction colorimetry output. Albeit important, scaling micrometer patterns carrying numerous also function amplifying units, signifying output signal. The detection limit assay reach 10.5 nM mode 18.7 mode, respectively. Furthermore, merits harvesting different generators multimodal readout allow mutual verification make analytical results more reliable. Collectively, our proposed may offer new idea combining inherent dual generators, emphasize accurate capability

Language: Английский

---

Unlocking of Hidden Mesopores for Enzyme Encapsulation by Dynamic Linkers in Stable Metal‒Organic Frameworks

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(51)

Published: Aug. 23, 2024

Mesoporous metal-organic frameworks (MOFs) are promising supports for the immobilization of enzymes, yet their applications often limited by small pore apertures that constrain size encapsulated enzymes to below 5 nm. In this study, we introduced labile linkers (4,4',4''-(2,4,6-boroxintriyl)-tribenzoate, TBTB) with dynamic boroxine bonds into mesoporous PCN-333, resulting in PCN-333-TBTB enhanced enzyme loading and protection capabilities. The selective breaking B-O creates defects which effectively expands both window cavity sizes, thereby unlocking hidden mesopores encapsulation. Consequently, strategy not only increases adsorption kinetics (<5 nm) such as cytochrome c (Cyt C) horseradish peroxidase (HRP), but also enables various large-sized (>5 nm), glycoenzymes. glycoenzymes@PCN-333-TBTB platform was successfully applied synthesize thirteen complex oligosaccharides polysaccharides, demonstrating high activity stability. linker-mediated encapsulation exceeding inherent MOFs, thus broadening scope enzymatic catalytic reactions achievable MOF materials.

Language: Английский

---

Cellulose Nanocrystal-Immobilized Lipase for Pickering Interface Biocatalysis

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Enzymes are promising biocatalysts due to their high efficiency, mild conditions, specificity, nontoxicity, and environmental friendliness. However, the enzyme suffers from poor stability reusability. Enzyme immobilization is a commonly used technology enhance its In this study, cellulose nanocrystal (CNC) was chosen as carrier sustainability, specific surface area, biocompatibility, water dispersibility, excellent Pickering emulsifying ability. Lipase immobilized on polydopamine-coated CNC (PC), obtained lipase-immobilized PC (LPC) displayed significantly enhanced higher activity in harsh conditions compared free lipase. LPC possessed partial wettability with both oil phases lower absolute value of zeta potential than CNC, endowing it better ability CNC. fixed at oil–water interface emulsion by ultrasonication, resulting droplets were employed microreactors for ester hydrolysis synthesis via biocatalysis. catalysis lipase traditional biphasic system increased contact area between substrate, reduced diffusion distance activation effect. Moreover, could be facilely recycled centrifugation, still retains activity. This study proposed facile sustainable method reusability achieved

Language: Английский

---

U-type π-conjugated phosphorescent ligand sensitized lanthanide metal–organic frameworks for efficient white-light-emitting diodes

Dalton Transactions, Journal Year: 2023, Volume and Issue: 52(39), P. 13872 - 13877

Published: Jan. 1, 2023

Lanthanide metal-organic framework (Ln-MOF) based phosphors for light-emitting diodes (LEDs) play an important role in the fields of solid-state lighting and display. The rational design organic antennae to address drawback low extinction coefficients lanthanide ions is highly desired. In this work, we provide a new strategy achieve energy transfer molecule with through-space conjugated folded structure, which can strengthen skeleton rigidity facilitate triplet state transfer. Consequently, one U-type π-conjugated 2,6-bis(3,5-dicarboxylphenoxy) pyridine (H4L) was selected as light gatherer sensitize construction Ln-MOFs [Ln(HL)(H2O)3]n (Eu-MOF Tb-MOF), exhibit long-lived luminescence lifetime (0.88 ms Eu-MOF 1.31 Tb-MOF) high quantum yields (50.87% 85.64% Tb-MOF). Furthermore, white LED device colour rendering index (89) fabricated using mixture commercial blue phosphor.

Language: Английский

---

The trade-off anionic modulation in metal–organic glasses showing color-tunable persistent luminescence

Materials Horizons, Journal Year: 2024, Volume and Issue: 11(20), P. 4951 - 4960

Published: Jan. 1, 2024

Ultralong room-temperature phosphorescence (RTP) and thermally activated delayed fluorescence (TADF) materials provide exciting opportunities for the rational design of persistent luminescence owing to their long-lived excitons.

Language: Английский

---