Conceptually innovative fluorophores for functional bioimaging DOI Creative Commons

Daniela Ceballos-Ávila,

Ixsoyen Vázquez-Sandoval,

Fernanda Ferrusca-Martínez

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116638 - 116638

Published: Aug. 9, 2024

Fluorophore chemistry is at the forefront of bioimaging, revolutionizing visualization biological processes with unparalleled precision. From serendipitous discovery mauveine in 1856 to cutting-edge fluorophore engineering, this field has undergone transformative evolution. Today, synergy chemistry, biology, and imaging technologies produced diverse, specialized fluorophores that enhance brightness, photostability, targeting capabilities. This review delves into history innovation fluorescent probes, showcasing their pivotal role advancing our understanding cellular dynamics disease mechanisms. We highlight groundbreaking molecules applications, envisioning future breakthroughs promise redefine biomedical research diagnostics.

Language: Английский

Matthew effect: General design strategy of ultra‐fluorogenic nanoprobes with amplified dark–bright states in aggregates DOI Creative Commons
S. Segawa, Xinwen Ou, Tianruo Shen

et al.

Aggregate, Journal Year: 2024, Volume and Issue: 5(2)

Published: Jan. 4, 2024

Abstract Fluorescence imaging, a key technique in biological research, frequently utilizes fluorogenic probes for precise imaging living systems. Tetrazine is an effective emission quencher probe designs, which can be selectively damaged upon bioorthogonal click reactions, leading to considerable enhancement. Despite significant efforts increase the enhancement ratio ( I AC / BC ) of tetrazine‐functionalized probes, influence molecular aggregation on properties has been largely overlooked these designs. In this study, we reveal that ultrahigh realized aggregate system when tetrazine paired with aggregation‐induced (AIE) luminogens. amplifies its quenching efficiency and drastically reduce background emissions. Subsequent reactions damage trigger AIE, considerably enhanced . We further showcase capability ultra‐fluorogenic systems selective multiple organelles cells. term unique fluorogenicity AIE luminogen‐quencher complexes amplified dark‐bright states as “Matthew effect” emission, potentially providing universal approach attain diverse

Language: Английский

Citations

10
Design strategies for tetrazine fluorogenic probes for bioorthogonal imaging DOI
Aiwen Yu, Xinyu He, Tianruo Shen

et al.

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In this review, recent progress in tetrazine-based fluorogenic probes for bioorthogonal chemistry is comprehensively analyzed, with a focus on advancements probe design and their imaging applications molecular processes live cells.

Language: Английский

Citations

1
Multiplex Detection of Biomarkers Empowered by Nanomaterials DOI Creative Commons
Zongbo Li, Mingquan Guo, Wenwan Zhong

et al.

Precision Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Language: Английский

Citations

0
Applications of Aggregation-Induced Emission Materials in Immunology: From Diagnostics to Immunotherapy DOI Creative Commons

Langyi Yang,

Ling‐Hong Xiong, Xuewen He

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Aggregation-induced emission (AIE) materials, due to their unique ability significantly enhance optical in aggregated states, have demonstrated vast potential immunology, particularly early disease diagnosis and immunotherapy. AIE materials can serve as highly efficient fluorescent probes for biomarker detection, providing critical insights diagnosis. Additionally, they act cancer vaccines, enhancing the effectiveness of immunotherapy through photodynamic therapy, photothermal or activation immune cells. This review highlights applications focusing on recent advancements diagnostics while also discussing challenges face directions future development.

Language: Английский

Citations

0
Fluorogenic Tetrazine Bioorthogonal Probes for Advanced Application in Bioimaging and Biomedicine DOI Creative Commons
Wuyu Mao, Ping Dong, Wei Du

et al.

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

A variety of bioorthogonal chemical tools have been developed and widely used in the study biological phenomena situ. Tetrazine chemistry exhibits ultrafast reaction kinetics, excellent biocompatibility, precise optical regulatory capabilities. Fluorogenic tetrazine probes achieved particularly diverse applications bioimaging disease diagnosis treatment. This Viewpoint briefly introduces characteristics advantages chemistry, some design strategies fluorogenic probes, status these to vivo imaging, as well Finally, we discuss challenges propose future trends field probes. offers insights into development new for biology research drugs.

Language: Английский

Citations

2
Conceptually innovative fluorophores for functional bioimaging DOI Creative Commons

Daniela Ceballos-Ávila,

Ixsoyen Vázquez-Sandoval,

Fernanda Ferrusca-Martínez

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116638 - 116638

Published: Aug. 9, 2024

Fluorophore chemistry is at the forefront of bioimaging, revolutionizing visualization biological processes with unparalleled precision. From serendipitous discovery mauveine in 1856 to cutting-edge fluorophore engineering, this field has undergone transformative evolution. Today, synergy chemistry, biology, and imaging technologies produced diverse, specialized fluorophores that enhance brightness, photostability, targeting capabilities. This review delves into history innovation fluorescent probes, showcasing their pivotal role advancing our understanding cellular dynamics disease mechanisms. We highlight groundbreaking molecules applications, envisioning future breakthroughs promise redefine biomedical research diagnostics.

Language: Английский

Citations

1