Organic probes for three‐photon fluorescence imaging in NIR‐II window: Design, applications, and perspectives DOI Creative Commons
Yuliang Yang,

Cui Cen,

Lijun Kan

et al.

Interdisciplinary materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 7, 2024

Abstract Three‐photon fluorescence (3PF) imaging is an emerging technology for deep‐tissue submicroscopic structures by nonlinearly redshifting the excitation wavelength to second near‐infrared (NIR‐II) window; thus, this approach has great advantages, including deep penetration depth, good spatial resolution, low background, and a high signal‐to‐noise ratio. 3PF been demonstrated be powerful tool noninvasively visualizing all kinds of tissues in recent years. Benefiting from excellent biosecurity structural controllability, development organic probes highly important advancing vivo. However, there no summary generalizability design progress probes. Herein, review introduces fundamental principle highlights advantages bioimaging. The molecular these also summarized based on relative optical indices. Furthermore, different application scenarios are listed detail. In end, main challenges, significance probe exploitation, prospective orientation precise proposed discussed promoting future applications clinical translation.

Language: Английский

Dual‐Locked Fluorescent Probes Activated by Aminopeptidase N and the Tumor Redox Environment for High‐Precision Imaging of Tumor Boundaries DOI
Yang Shen, Wei Li, Zhixuan Zhou

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(32)

Published: May 23, 2024

Clear delineation of tumor margins is essential for accurate resection and decreased recurrence rate in the clinic. Fluorescence imaging emerging as a promising alternative to traditional visual inspection by surgeons intraoperative imaging. However, probes lack accuracy diagnosis, making it difficult depict boundaries accurately. Herein, we proposed an offensive defensive integration (ODI) strategy based on "attack systems (invasive peptidase) defense (reductive microenvironment)" multi-dimensional characteristics design activatable fluorescent precisely. Screened out from series ODI strategy-based probes, ANQ performed better than unilateral correlation distinguishing between cells normal minimizing false-positive signals living metabolic organs. To further improve signal-to-background ratio vivo, derivatized FANQ, was prepared successfully applied distinguish orthotopic hepatocellular carcinoma tissues adjacent mice models clinical samples. This work highlights innovative develop rapid diagnosis tumors high-precision boundaries, providing more efficient tools future applications assisted resection.

Language: Английский

Citations

21
A Dual-Cascade Activatable Near-Infrared Fluorescent Probe for Precise Intraoperative Imaging of Tumor DOI

Yan–Bin Feng,

Haohao Yan,

Xiaocheng Mou

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(20), P. 6131 - 6138

Published: May 10, 2024

Accurate intraoperative tumor delineation is critical to achieving successful surgical outcomes. However, conventional techniques typically suffer from poor specificity and low sensitivity are time-consuming, which greatly affects decision-making. Here, we report a cascade activatable near-infrared fluorescent (NIRF) probe IR780SS@CaP that can sequentially respond acidity elevated glutathione levels for accurate localization. Compared with nonactivatable single-factor probes, strategy minimize nonspecific activation false positive signals in complicated biological environment, affording superior tumor-to-normal tissue ratio facilitate the of abdominal metastases. Small metastatic lesions were less than 1 mm diameter be precisely identified by completely excised under NIRF imaging guidance. This study could benefit diagnosis image-guided surgery providing real-time information reliable decision support, thus reducing risk both recurrence complications improve patient

Language: Английский

Citations

11
“Zero” Intrinsic Fluorescence Sensing‐Platforms Enable Ultrasensitive Whole Blood Diagnosis and In Vivo Imaging DOI
Gangwei Jiang, Hong Liu,

Guohui Deng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(16)

Published: Feb. 27, 2024

Abnormal physiological processes and diseases can lead to content or activity fluctuations of biocomponents in organelles whole blood. However, precise monitoring these abnormalities remains extremely challenging due the insufficient sensitivity accuracy available fluorescence probes, which be attributed background arising from two sources, 1) biocomponent autofluorescence (BCAF) 2) probe intrinsic (PIF). To overcome obstacles, we have re-engineered far-red NIR II rhodol derivatives that possess weak BCAF interference. And a series "zero" PIF sensing-platforms were created by systematically regulating open-loop/spirocyclic forms. Leveraging advancements, devised various ultra-sensitive indicators, achieving substantial boosts (190 1300-fold). Among 8-LAP demonstrated accurate tracking quantifying leucine aminopeptidase (LAP) blood at stages tumor metastasis. Furthermore, coupling with an endoplasmic reticulum-targeting element enabled detection ERAP1 HCT116 cells p53 abnormalities. This delicate design eliminating provides insights into enhancing existing probes toward imaging abnormal diseases.

Language: Английский

Citations

10
Engineering a far-red fluorescent probe for rapid detection of Hg(II) ions in both cells and zebrafish DOI

Patthavongsa Patthana,

Hai-Chen Zhong,

Qian Wu

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 318, P. 124469 - 124469

Published: May 16, 2024

Language: Английский

Citations

9
Xanthene-Based NIR organic phototheranostics agents: Design strategies and biomedical applications DOI
Xiao‐Yun Ran, Yuanfeng Wei, Yanling Wu

et al.

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Near-infrared fluorescence imaging is key in biological exploration. Short emission wavelengths of xanthene dyes limit their use. Structural modifications shift emissions to the NIR-I/II range, enhancing biomedical applications.

Language: Английский

Citations

1
Precision Molecular Engineering of Compact Near-Infrared Fluorophores DOI
Rongrong Huang, Qinglong Qiao, Deborah Seah

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Organic fluorophores with near-infrared (NIR) emission and reduced molecular size are crucial for advancing bioimaging biosensing technologies. Traditional methods, such as conjugation expansion heteroatom engineering, often fail to reduce fluorophore without sacrificing NIR properties. Addressing this challenge, our study utilized quantum chemical calculations structure-property relationship analysis establish an iterative design approach enable precision engineering compact, single-benzene-based fluorophores. These newly developed exhibit emissions up 759 nm maintain weights low 192 g/mol, approximately 50% of that Cy7. Additionally, they display unique environmental sensitivity─nonemissive in aqueous solutions but highly emissive lipid environments. This property significantly enhances their utility wash-free imaging live cells. Our findings mark a substantial breakthrough paving the way more efficient adaptable methodologies.

Language: Английский

Citations

1
Recent advances in minimal fluorescent probes for optical imaging DOI Creative Commons

Fabio De Moliner,

Ferran Nadal‐Bufí, Marc Vendrell

et al.

Current Opinion in Chemical Biology, Journal Year: 2024, Volume and Issue: 80, P. 102458 - 102458

Published: April 25, 2024

Fluorescent probes have revolutionized biological imaging by enabling the real-time visualization of cellular processes under physiological conditions. However, their size and potential perturbative nature can pose challenges in retaining integrity functions. This manuscript highlights recent advancements development small fluorescent for optical studies. Single benzene-based fluorophores offer versatility with minimal disruption, exhibiting diverse properties like aggregation-induced emission pH responsiveness. nucleobases enable precise labeling nucleic acids without compromising function, offering high sensitivity compatibility biochemistry Bright yet amino provide an interesting alternative to bulky fusion proteins, facilitating non-invasive events precision. These miniaturized promise enhanced capabilities studying systems a manner, fostering further innovations molecular imaging.

Language: Английский

Citations

9
Cancer-Targeting and Viscosity-Activatable Near-Infrared Fluorescent Probe for Precise Cancer Cell Imaging DOI
Ming Qian, Ye Yuan, Tian‐Bing Ren

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(33), P. 13447 - 13454

Published: Aug. 9, 2024

Small-molecule fluorescent probes have emerged as potential tools for cancer cell imaging-based diagnostic and therapeutic applications, but their limited selectivity poor imaging contrast hinder broad applications. To address these problems, we present the design construction of a novel near-infrared (NIR) biotin-conjugated viscosity-activatable probe, named QL-VB, selective recognition cells. The designed probe exhibited NIR emission at 680 nm, with substantial Stokes shift 100 nm remarkably sensitive responses toward viscosity changes in solution. Importantly, QL-VB provided an evidently enhanced signal-to-noise ratio (SNR: 6.2) discrimination cells/normal cells, compared control without biotin conjugation 1.8). Moreover, validated capability dynamic monitoring stimulated within cells employed distinguishing breast tissues from normal live mice improved accuracy 2.5) comparison All findings indicated that cancer-targeting not only enables mechanistic investigations mitochondrial alterations also holds robust tool

Language: Английский

Citations

8
Inhaled NIR‐II Nanocatalysts for Real‐Time Monitoring and Immunomodulatory Therapy of Acute Lung Injury DOI
Yi Li, Dongfang Liu, Ting Chen

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 13, 2024

Abstract Acute lung injury (ALI) poses a significant and escalating medical challenge, where precise diagnosis timely intervention hold utmost importance in halting its deterioration. Nevertheless, persistent obstacles arise from the lack of agents proficient both real‐time efficient mitigation ALI. Here, biocatalytic second near‐infrared (NIR‐II) fluorescence‐illuminating nanoplatform is designed to simultaneously facilitate monitoring robust inflammation alleviation The study first develops new aggregation‐induced emission luminogen with trifluoromethyl substitutions, which increase NIR‐II wavelength fluorescence brightness. molecular probe further integrated into hollow ceria nanostructures, cloaked pre‐activated macrophage membranes for targeted intervention. Upon inhalation administration ALI mice, theranostic nanoagents leverage bright active inflammation‐tropic properties vivo sensitive imaging ALI, also facilitates tracking nanoagents’ distribution dynamic fate within pneumonia milieu. Concurrently, catalytic prowess nanoplatforms efficaciously scavenges excess reactive oxygen species, dampens proinflammatory cytokines, promotes repolarization, substantially alleviating acute damage. multifaceted integrates bioimaging nanocatalysis‐mediated immunoregulation, offering versatile promising approach addressing intricate challenges posed by diseases.

Language: Английский

Citations

7
A minireview of fluorescent probes for the dual detection of viscosity and pH: Design and biological applications DOI

Wenyu Xi,

Peikun Xiao,

Hong Huang

et al.

Dyes and Pigments, Journal Year: 2024, Volume and Issue: 231, P. 112412 - 112412

Published: Aug. 16, 2024

Language: Английский

Citations

7