Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115890 - 115890
Published: May 1, 2025
Language: Английский
Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)
Published: March 16, 2024
Abstract Conventional cancer treatments can cause serious side effects because they are not specific to cells and damage healthy cells. Aptamers often single-stranded oligonucleotides arranged in a unique architecture, allowing them bind specifically target sites. This feature makes an ideal choice for targeted therapeutics. They typically produced through the systematic evolution of ligands by exponential enrichment (SELEX) undergo extensive pharmacological revision modify their affinity, specificity, therapeutic half-life. act as drugs themselves, directly inhibiting tumor Alternatively, be used drug delivery systems transport cells, minimizing toxicity In this review, we will discuss latest most advanced approaches using aptamers treatment, particularly therapy overcoming resistance conventional therapies.
Language: Английский
Citations
34
International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 2427 - 2443
Published: Feb. 1, 2025
The increasing global prevalence of cardiovascular diseases highlights the urgent need for innovative diagnostic and therapeutic strategies. Aptamers, small single-stranded nucleic acid molecules with exceptional specificity affinity target biomolecules, have emerged as promising tools precise diagnostics targeted therapies. Their selective binding capabilities provide valuable insights into molecular mechanisms underlying conditions. When integrated nanosystems, aptamers enhance delivery, bioavailability, stability agents, addressing challenges solubility degradation. This integration enables more drug advanced imaging techniques, improved interventions, ultimately improving management diseases. Recent advancements in aptamer selection methodologies, coupled their unique three-dimensional structures, significantly expanded application potential health. By combining novel approaches to disease diagnosis treatment are emerging, enhanced efficacy, safety, precision. review explores recent progress development aptamer-based nanosystems
Language: Английский
Citations
2
Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
The affinity of nucleic acid aptamers isolated in vitro via Systematic Evolution Ligands by Exponential Enrichment (SELEX) is often limited because the entire potential sequence space cannot be screened. In this study, we introduce Motif-SELEX, a novel method that enables optimization existing underperforming generating libraries broadly represent both and length variations parent sequence. This approach isolation sequences with improved without biases limitations traditional mutagenesis methods like doped SELEX error-prone PCR. As demonstration, applied Motif-SELEX to DNA-based morphine aptamer 2' fluoro- methoxy-RNA-based apixaban aptamer, discovering new, better-performing differing random domain lengths from their parents up 10-fold improvements affinity. These new would inaccessible post-SELEX methods. Critically, our analysis pools also enabled us identify structural elements crucial for target binding predict secondary tertiary structures given family─even when those involve noncanonical nucleotide interactions. We believe offers an effective generalizable solution optimizing structure properties functional molecules diverse applications.
Language: Английский
Citations
2
Biosensors, Journal Year: 2024, Volume and Issue: 14(5), P. 210 - 210
Published: April 23, 2024
The rapid and sensitive detection of pathogenic bacteria is becoming increasingly important for the timely prevention contamination treatment infections. Biosensors based on nucleic acid aptamers, integrated with optical, electrochemical, mass-sensitive analytical techniques, have garnered intense interest because their versatility, cost-efficiency, ability to exhibit high affinity specificity in binding bacterial biomarkers, toxins, whole cells. This review highlights development structural characterization, chemical modifications enabling optimized recognition properties enhanced stability complex biological matrices. Furthermore, recent examples aptasensors cells, toxins are discussed. Finally, we explore barriers discuss perspectives application aptamer-based detection.
Language: Английский
Citations
15
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(25), P. 10391 - 10398
Published: June 6, 2024
DNA-templated silver nanoclusters (AgNCs-DNA) can be synthesized via a one-pot method bypassing the tedious process of biomolecular labeling. Appending an aptamer to DNA templates results in dual-functionalized strands that utilized for synthesizing aptamer-modified AgNCs, thereby enabling development label-free fluorescence aptasensors. However, major challenge lies necessity redesign strand each specific target, thus increasing complexity and hindering widespread application these To overcome this challenge, we designed six (DNA1-DNA6) incorporate AgNCs synthesis A4-linker further coupling. Among all AgNCs-DNA samples, it was found both AgNCs-DNA1 AgNCs-DNA2 stood out their excellent long-term stability. After capturing T4-linker connected with aptamer1 aflatoxin B1 (AFB1), however, only AgNCs-DNA1/aptamer1 maintained This finding highlighted potential as versatile probe on-demand emphasize its benefits aptasensing applications, MoS
Language: Английский
Citations
9
Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112695 - 112695
Published: Jan. 1, 2025
Language: Английский
Citations
1
Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 29 - 29
Published: Jan. 8, 2025
Nucleic acid aptamers are single-stranded oligonucleotides that selected through exponential enrichment (SELEX) technology from synthetic DNA/RNA libraries. These can specifically recognize and bind to target molecules, serving as specific recognition elements. Surface-enhanced Raman scattering (SERS) spectroscopy is an ultra-sensitive, non-destructive analytical technique rapidly acquire the “fingerprint information” of measured molecules. It has been widely applied in qualitative trace analysis across various fields, including food safety, environmental monitoring, biomedical applications. Small such toxins, antibiotics, pesticides, have significant biological effects harmful both human health environment. In this paper, we mainly introduced application research progress SERS detection with (aptamer-based techniques) field small-molecule detection, particularly pesticide (animal) residues, toxins. And prospect combining two methods were reviewed.
Language: Английский
Citations
1
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
Glycoproteins are of significant value to liquid biopsy human diseases. Herein, we present a universal electrochemical platform for the amplified detection glycoproteins, taking advantage glycan-matchmade multivalent decoration enzyme labels enzymatic signal amplification. Briefly, involves two steps, i.e., site-directed phenylboronic acid-coated gold nanoparticles (PBA-AuNPs) cis-diol-containing glycans glycoproteins and subsequent via affinity cross-linking between glycan moieties remaining PBA groups on PBA-AuNP cross-linkers. As matchmaking-based strategy enables each glycoprotein with multiple labels, this exhibits high sensitivity toward detection. Using alkaline phosphatase (ALP) as proof-of-concept label in combination solid-state voltammetric stripping assay enzymatically deposited metallic silver, limits at pg mL-1 level have been obtained aptamer-based thrombin prostate-specific antigen. Overall, work illustrates an efficient versatile ultralow concentration levels, holding desirable advantages simplicity cost-effectiveness over sandwich enzyme-linked immunosorbent assays.
Language: Английский
Citations
1
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 12, 2025
Ensuring food safety is a shared responsibility across the entire supply chain, encompassing manufacturers, processors, retailers, consumers, and regulatory bodies. However, traditional detection methods have several limitations, including slow processing times, high costs, limited sensitivity, susceptibility to false positives or negatives. These shortcomings underscore urgent need for faster, more accurate, cost-effective technologies. Aptamers aptasensors emerged as promising alternatives. offer advantages over recognition probes due their affinity specificity diverse targets. The enable rapid detection, cost reduction, shelf life extension, minimal batch-to-batch variability, making them highly suitable applications. Detecting small molecules such toxins, antibiotics, pesticides, contaminants, heavy metals remains challenging steric hindrance, nonspecific binding, reduced accuracy. Recent advancements in aptamer technology focused on pre- postmodifications enhance performance. One of most innovations development split aptamers. engineered aptamers, designed operate segments known improved flexibility binding specificity, effectively addressing challenges detecting small-sized This review examines evolution aptamers aptasensors, focusing application that are essential safety. It reported strategies modifying optimizing selected providing details developing approach address unique small-molecule detection. Additionally, recent its integration into aptasensor highlighted, showcasing how these revolutionizing hazards by overcoming limitations methods.
Language: Английский
Citations
1
Biomaterials, Journal Year: 2025, Volume and Issue: 319, P. 123180 - 123180
Published: Feb. 13, 2025
Language: Английский
Citations
1