Activatable fluorescent probes for imaging and diagnosis of hepatocellular carcinoma DOI Creative Commons

Xiaoxiao Men,

Fanghong Liu, Maolian Gong

et al.

Journal of Innovative Optical Health Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Liver cancer, specifically hepatocellular carcinoma (HCC), is a malignant neoplasm of the digestive system, characterized by exceptionally high morbidity and mortality rates on global scale. Early detection diagnosis are critical measures for enhancing prognosis patients diagnosed with HCC. An improved significantly reliant timely disease effective therapeutic monitoring. Activatable fluorescent probes essential detecting imaging biomarkers related to in vivo imaging. This paper reviews developed over past five years noninvasive optical modality demonstrates significant promise targeting pathological sites anticipated facilitate potential clinical translation.

Language: Английский

Imaging-guided Companion Theranostics: Ushering in A New Era of Personalized Cancer Care DOI Creative Commons
Meng Li, Yumeng Wu, Jing Lin

et al.

Nano Biomedicine and Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

1
Carboxylesterase-Triggered Theranostic Agent: Advancing Near-Infrared Imaging and Therapeutic Efficacy in Hepatocellular Carcinoma DOI
Fan Zheng,

Heying Yao,

Duoyang Fan

et al.

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Hepatocellular carcinoma (HCC) poses a global challenge due to the lack of accurate early stage detection methods. Carboxylesterase (CE), key HCC biomarker, presents an ideal target for diagnosis. Herein, we developed novel CE-sensitive fluorescent probe (HFP-CE) that offered powerful near-infrared fluorescence signals and potent synergistic photodynamic/chemodynamic therapeutic effect against HCC. This was synthesized by linking ferrocenecarboxylic acid (a Fenton reagent) HFP-OH photosensitizer) through self-eliminating spacer. activation enabled ratiometric imaging CE, allowing specific differentiation cells from others, exceeding capabilities commercial kits. Moreover, HFP-CE could generate 1O2 •OH upon CE in vitro, triggering both apoptosis ferroptosis cancer cells. Remarkably, real-time tumor visualization effective growth inhibition vivo. study showcased promise as versatile tool advancing precision medicine

Language: Английский

Citations

0
NIR‐II Fluorescence/Photoacoustic Dual Ratiometric Probes with Unique Recognition Site for Quantitatively Visualizing H2S2 in Vivo DOI Open Access

Bangping Hu,

Qi Liu,

Yulan Jiang

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Hydrogen persulfide (H

Citations

3
Amphiphilic Azulene-Based Fluorescent Probe for Simultaneous Monitoring of Fluctuations in Carboxylesterase Activity in Diverse Biological Samples from a Single Organism DOI Creative Commons
Zhenling Cui, Yafu Wang, Ge Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19732 - 19739

Published: Nov. 25, 2024

Carboxylesterase (CEs), as a key enzyme in ester metabolism, is simultaneously found with varying expression levels diverse biological samples from single organism, such tissues, cells, bacteria and blood. However, the lack of integrated universal tools for comprehensive detection CEs'activity fluctuations organism severely hinders diagnosis treatment related diseases. Herein, we have developed an amphiphilic fluorescent probe (AZU-β) targeted toward CEs using azulene derivative (AZU-OH) fluorophore. Using "hydroxyl protection–deprotection" strategy, AZU-β incorporates specific recognition group (acetyl ester) that activates intramolecular charge transfer process to regulate signal CEs. exhibits selectivity highly sensitivity (the minimum limit 1.8 × 10–2 U/mL), well rapid response (within approximately 6.0 s), detecting over wide range U/mL 1.0 U/mL. Moreover, outstanding water–oil amphiphilicity which makes it suitable different biomembrane permeability levels. Therefore, serves tool can not only detect at serum level but also cellular, tissue bacterial under drug-induced liver injury conditions enabling simultaneous monitoring organism. It expected more probes targeting various disease-associated enzymes be designed based on this design strategy monitor relevant activity providing advanced analytical pathological research diagnosis.

Language: Английский

Citations

1
Fluorogenic Probes for Functional Imaging of Endoplasmic Reticulum‐Resident Proteins: from Molecular Engineering to Biomedical Applications DOI Open Access
Yufan Fan, Feng Zhang,

Yumeng Hao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Endoplasmic reticulum‐resident proteins (ERPs) are crucial for maintaining cellular homeostasis and tightly associated with the onset progression of various diseases. The emergence fluorogenic probes responsive to ERPs has enabled scientists in situ monitor spatial distribution function status target real‐time, which is very helpful elucidating ERP‐related pathological mechanisms developing innovative therapeutics. Over past decade, a range sensing imaging ERPs, including ion channels, oxidases, hydrolases, transferases, have been devised. This review systematically summarizes recent advances molecular engineering biomedical applications ERP probes. Engineering strategies constructing ERP‐activatable probes, along their structural features, recognition fragments, performances, discussed. Meanwhile, these biomarker analysis, disease treatment, multimodal imaging, drug discovery also highlighted. Finally, forward‐looking perspective on future directions underlying challenges explored. insights knowledge presented here will spur further innovation technological breakthroughs multifunctional advancing diagnosis, ER biology, discovery.

Language: Английский

Citations

1
Nanoprobe Based on Novel NIR‐II Quinolinium Cyanine for Multimodal Imaging DOI

Meilin Xu,

Yanhong Lin, Yuhang Li

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Abstract NIR‐II imaging has the advantages of high sensitivity, spatiotemporal resolution, and penetration depth, thereby serving as a potential alternative to conventional methods. Herein, novel dye IR‐1010 ( λ ex / em = 1010/1058 nm) is reported with quantum yield (3.08%) good stability, by incorporating p‐methoxyphenyl groups into quinolinium cyanine dye. Then multifunctional nanoprobe, termed IUFP NPs , developed incorporation upconversion (UC) nanoparticles (NPs), perfluoro‐15‐crown‐5‐ether (PFCE), IR‐1010, display performance multimodal imaging. Under single‐wavelength excitation (980 nm), simultaneously emit fluorescence visible UC luminescence UCNPs, thus realize for cells, fluorescence/photoacoustic/ 19 F magnetic resonance blood vessels, lymph nodes tumor in mice. This work affords approach dyes general strategy design probes.

Language: Английский

Citations

0
NIR‐II Fluorescence/Photoacoustic Dual Ratiometric Probes with Unique Recognition Site for Quantitatively Visualizing H2S2 in Vivo DOI Open Access

Bangping Hu,

Qi Liu,

Yulan Jiang

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Abstract Hydrogen persulfide (H 2 S ) plays a significant role in redox biology and signal transduction; therefore, quantitative visualization of H the deep tissue living organisms is essential for obtaining reliable information about relevant pathophysiological processes directly. However, currently reported probes are unsuitable this purpose because their poor selectivity many polysulfide species or short wavelength, which hinders precise imaging tissues. Herein, first time, we report unique ‐mediated dithiole formation reaction. Based on reaction, construct NIR‐II fluorescence (FL) photoacoustic (PA) dual‐ratiometric probe ( NIR‐II‐H quantitatively visualizing vivo. This shows (I 840 /I 1000 , 107‐fold) (PA 800 /PA 900 6.5‐fold) responses towards Na with high specificity, excellent sensitivity (1.8 nM), improved water solubility, deep‐tissue penetration. More importantly, using FL/PA imaging, successfully demonstrated that could be used to accurately quantify fluctuating levels liver‐injury mouse models induced by lipopolysaccharides metformin drugs. Overall, study not only presents promising tool ‐related pathological research, but also provides recognition site may generalized designing more useful agents future.

Language: Английский

Citations

0
Activatable fluorescent probes for imaging and diagnosis of hepatocellular carcinoma DOI Creative Commons

Xiaoxiao Men,

Fanghong Liu, Maolian Gong

et al.

Journal of Innovative Optical Health Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Liver cancer, specifically hepatocellular carcinoma (HCC), is a malignant neoplasm of the digestive system, characterized by exceptionally high morbidity and mortality rates on global scale. Early detection diagnosis are critical measures for enhancing prognosis patients diagnosed with HCC. An improved significantly reliant timely disease effective therapeutic monitoring. Activatable fluorescent probes essential detecting imaging biomarkers related to in vivo imaging. This paper reviews developed over past five years noninvasive optical modality demonstrates significant promise targeting pathological sites anticipated facilitate potential clinical translation.

Language: Английский

Citations

0