Sensitive Fluorescent Determination of Matrix Metalloproteinase-2 (MMP-2) Using the Inner Filter Effect (IFE) with Carbon Dots and Aggregated Gold Nanoparticles DOI
Dapeng Sun, Chenglong Ye, Zhuo Chen

et al.

Analytical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Dec. 5, 2024

Matrix metalloproteinase-2 (MMP-2) is a key biomarker involved in various pathological processes, so its sensitive and selective detection of great clinical significance. A fluorescent sensor for the MMP-2 was successfully designed based on inner filter effect (IFE) between carbon dots (CDs) gold nanoparticles (AuNPs). In presence MMP-2, peptide chains are hydrolyzed, releasing short peptides that contain thiol groups positive charges, thereby inducing aggregation AuNPs. This disrupts IFE AuNPs CDs, leading to significant enhancement fluorescence. By monitoring change fluorescence intensity concentration accurately determined. The exhibited linear response across range from 1.0 150 ng/mL, with limit as low 0.23 which lower than those previously reported methods. Furthermore, demonstrated minimal interference coexisting biochemical substances human serum. Notably, applied determine serum, showing satisfactory recovery rates ranging 98.8 1106.0%. conclusion, this offers high selectivity sensitivity detection, making it promising tool diagnosis related diseases.

Language: Английский

Highly Photoreactive Semiconducting Polymers with Cascade Intramolecular Singlet Oxygen and Energy Transfer for Cancer-Specific Afterglow Theranostics DOI
Yiguang Lin, Jingsheng Huang, Jing Liu

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Afterglow luminescence provides ultrasensitive optical detection by minimizing tissue autofluorescence and increasing the signal-to-noise ratio. However, due to lack of suitable unimolecular afterglow scaffolds, current agents are nanocomposites containing multiple components with limited performance have rarely been applied for cancer theranostics. Herein, we report synthesis a series oxathiine-containing donor-acceptor block semiconducting polymers (PDCDs) observation their high photoreactivity strong near-infrared (NIR) luminescence. We reveal that PDCDs absorb NIR light undergo photodynamic process generate singlet oxygen (1O2), which intramolecularly transfers efficiently reacts oxathiine form intermediates low Gibbs free energy changes required this photoreaction. Following intramolecular transfer from donor acceptor block, emission is produced PDCDs. Owing efficient cascade photochemical process, PDCD-based nanoparticles achieve higher brightness longer compared most reported agents, even after ultrashort photoirradiation only 3 s. Furthermore, within PDCD can be inhibited bioconjugation quencher-linked peptide. This allows construction cancer-activatable theranostic probe (CATP) switches on signal function in presence cancer-overexpressed enzyme. Thereby, CATP represents first phototheranostic permits cancer-specific therapy under preclinical settings. In summary, study molecular guideline develop probes photoreactive polymers.

Language: Английский

Citations

2

Recent advances in low‐background luminescent nanomaterials‐enhanced lateral flow assays for clinical diagnosis DOI Creative Commons

Liangwen Hao,

Jing Chen,

Quanshuang Li

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Abstract Lateral flow assays (LFAs) are widely applicable in clinical point‐of‐care testing (POCT) due to their unique advantages such as simplicity, rapidity, and cost‐effectiveness. However, sensitivity is often constrained by the background autofluorescence of biological sample, self‐matrix, or readout technique, thereby leading overlooking trace amounts biomarkers present early disease. In recent years, various nanomaterials‐based methods have been developed address this issue, including time‐gated wavelength‐differentiated strategy external modulated for separation minimize interference from samples. This review provides a comprehensive overview low‐background luminescent nanoparticles (LBLNPs)‐enhanced LFA systems, focusing on analyzing underlying mechanism these nanomaterials improving accuracy LFAs platform diagnosis. Representative examples selected demonstrate potential detecting disease‐associated samples blood, urine, saliva et al. Finally, unresolved challenges future development prospects briefly discussed.

Language: Английский

Citations

1

Reactive oxygen species-mediated organic long-persistent luminophores light up biomedicine: from two-component separated nano-systems to integrated uni-luminophores DOI
Zhe Li, Hong‐Wen Liu, Xiaobing Zhang

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

An overview of the recent advances in reactive oxygen species-mediated organic long-persistent luminophores, including their history, working mechanisms, design strategies, and biomedical applications.

Language: Английский

Citations

5

Ultrabright difuranfluoreno-dithiophen polymers for enhanced afterglow imaging of atherosclerotic plaques DOI

Zhe Li,

Hui Cao,

Youjuan Wang

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(13)

Published: March 26, 2025

Cardiovascular diseases, including stroke driven by atherosclerosis, remain a leading global health concern. Current diagnostic imaging modalities such as magnetic resonance fail to characterize oxidative stress within atherosclerotic plaques. Here, we introduce difuranfluoreno-dithiophen–based polymers designed for afterglow imaging, offering ultrabright luminescence, ultralow-power excitation (0.087 milliwatts per square centimeter), and ultrashort acquisition times (0.01 seconds). Through molecular engineering strategy, have optimized enhanced reactive oxygen species (ROS) generation capability, ROS capturing fluorescence quantum yield, resulting in an increase intensity (~130-fold) compared commonly used 2-methoxy-5-(2′-ethylhexyloxy)-1,4-phenylenevinylene polymer (MEHPPV). Additionally, developed ratiometric nanoparticles doped with stress–responsive molecules, enabling of markers plaque. This approach provides tool cardiovascular diagnostics, which is conducive the auxiliary diagnosis risk stratification atherosclerosis.

Language: Английский

Citations

0

A dark-state–dominated photochemical upconversion afterglow via triplet energy transfer relay DOI Creative Commons

Hang Yuan,

Kuangshi Sun,

Xianlong Su

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(17)

Published: April 25, 2025

Photochemical afterglow materials have drawn considerable attention due to their attractive luminescent properties and great application potential. Considering the classical photochemical always exhibit poor luminescence, it is urgent gain fundamental understanding of main limiting factors. Here, we identified existence a dark-state triplet in process, an overwhelming percentage ~98.5% was revealed for this non-emissive state. Guided by these observations, proposed activate unprecedented energy transfer relay simultaneously harness singlet energy. Consequently, upconverted material constructed with amazing luminescence performance albeit its moderate fluorescence emission property. The generality strategy evidenced adaptation similar emitters varied wavelengths. optimized enabled time-gated upconversion bioimaging under ultralow-power excitation. This study not only reveals pathways but also paves way rational design bright ultralong lifetime.

Language: Английский

Citations

0

Sensitive Fluorescent Determination of Matrix Metalloproteinase-2 (MMP-2) Using the Inner Filter Effect (IFE) with Carbon Dots and Aggregated Gold Nanoparticles DOI
Dapeng Sun, Chenglong Ye, Zhuo Chen

et al.

Analytical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Dec. 5, 2024

Matrix metalloproteinase-2 (MMP-2) is a key biomarker involved in various pathological processes, so its sensitive and selective detection of great clinical significance. A fluorescent sensor for the MMP-2 was successfully designed based on inner filter effect (IFE) between carbon dots (CDs) gold nanoparticles (AuNPs). In presence MMP-2, peptide chains are hydrolyzed, releasing short peptides that contain thiol groups positive charges, thereby inducing aggregation AuNPs. This disrupts IFE AuNPs CDs, leading to significant enhancement fluorescence. By monitoring change fluorescence intensity concentration accurately determined. The exhibited linear response across range from 1.0 150 ng/mL, with limit as low 0.23 which lower than those previously reported methods. Furthermore, demonstrated minimal interference coexisting biochemical substances human serum. Notably, applied determine serum, showing satisfactory recovery rates ranging 98.8 1106.0%. conclusion, this offers high selectivity sensitivity detection, making it promising tool diagnosis related diseases.

Language: Английский

Citations

1