Intelligent Molecular Cleavage and Dual-Signal Relay Amplification Ratiometric Strategy for High-sensitivity Analysis and Dynamic Monitoring of Exosomal RNA in Glioma

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 274, P. 117205 - 117205

Published: Jan. 31, 2025

Language: Английский

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Supramolecular DNA Nanodevice Assembled via RCA and HCR Cascade Reaction on Tetrahedral DNA Nanostructure for Sensitive Detection and Intracellular Imaging of Dual-miRNAs Associated with Liver Cancer

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Herein, a supramolecular DNA nanodevice was formed via the rolling circle amplification (RCA) and hybridization chain reaction (HCR) cascade on tetrahedral nanostructure (TDN) to achieve simultaneous sensitive detection intracellular imaging of dual-miRNAs related liver cancer. The effectively addressed limitations low probe loading capacity in traditional TDN nanodevices by enriching plenty signal probes around single TDN, significantly enhancing fluorescence signal. Impressively, with fulcrum dense structure imparted strong rigidity, ensuring stability decrease aggregation quenching for further increasing response. Consequently, biosensor based enabled miRNA221 miRNA222, achieved accurate situ displaying improved capabilities compared TDN-based nanodevices. More importantly, precise miRNA222 could distinguish hepatocellular carcinoma cells different degrees metastasis from human normal cells, providing more information diagnosis development carcinoma.

Language: Английский

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DNA Tetrahedron-Driven Multivalent Proteolysis-Targeting Chimeras: Enhancing Protein Degradation Efficiency and Tumor Targeting

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Proteolysis-targeting chimeras (PROTACs) are dual-functional molecules composed of a protein interest (POI) ligand and an E3 ligase connected by linker, which can recruit POI ligases simultaneously, thereby inducing the degradation showing great potential in disease treatment. A challenge developing PROTACs is design linkers modification ligands to establish multifunctional platform that enhances efficiency antitumor activity. As programmable modifiable nanomaterial, DNA tetrahedron precisely assemble selectively recognize flexibly adjust distance between molecules, making them ideal linkers. Herein, we developed multivalent PROTAC based on tetrahedron, named AS-TD2-PRO. Using as combined modules targeting tumor cells, recognizing ligases, multiple together. We took undruggable target signal transducer activator transcription 3 (STAT3), associated with etiology progression variety malignant tumors, example this study. AS-TD2-PRO two STAT3 recognition demonstrated good enhancing tumor-specific compared traditional bivalent PROTACs. Furthermore, mouse model, superior therapeutic activity was observed. Overall, tetrahedron-driven both serve proof principle for introduce promising avenue cancer treatment strategies.

Language: Английский

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DNA Nanostructures‐Based In Situ Cancer Vaccines: Mechanisms and Applications

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Abstract Current tumor vaccines suffer from inadequate immune responsive due to the insufficient release of antigens, low infiltration, and immunosuppressive microenvironment. DNA nanostructures with their ability precisely engineer, controlled release, biocompatibility, capability augment immunogenicity microenvironment, have gained significant attention for potential revolutionize vaccine designing. This review summarizes various applications in construction situ cancer vaccines, which can generate tumor‐associated antigens directly damaged tumors immune‐stimulation. The mechanisms components are listed, specific strategies constructing using explored underlying action elucidated. immunogenic cell death (ICD) induced by chemotherapeutic agents, photothermal therapy (PTT), photodynamic (PDT), radiation (RT) related building systematically summarized. different immunotherapy elaborated, exerts precise, long‐lasting, robust responses. current challenges future prospectives proposed. provides a holistic understanding evolving role development.

Language: Английский

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A Trinity DNAzyme Circuit with Dual‐Confinement Effect for Imaging‐Guided Combined Gene/Chemo‐Dynamic Therapy

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Synthetic catalytic DNAzyme circuits have gained great attention as versatile theranostic toolboxes, but their accuracy and efficiency is restricted by uncontrolled signal leakage inefficient circuitry activation. Here, a trinity circuit (TriDC) constructed using unique logic gate bioswitchable DNA tetrahedral walker@MnO 2 nanosheets structure. The AND walker, composed of dual allosteric (termed “commander” “soldier”) scaffold, used to execute imaging‐guided gene therapy avoiding undesired cascade “walking” in confined space. Glutathione‐mediated reduction MnO provides secondary confinement stack the walker situ, offering abundant Mn 2+ cofactor achieve self‐sufficiency induce chemo‐dynamic therapy. structure increases local reaction concentrations ≈100‐fold according collision frequency model, enabling detection microRNA‐10b microRNA‐155 at limit 98.97 p m , thereby facilitating precise imaging breast cancer cells. combined gene/chemo‐dynamic achieves 66.2% tumor growth inhibition efficiency. TriDC offers novel insights into theranostics also presents paradigm for situ implementation higher‐order circuits.

Language: Английский

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DNA Framework-Enabled Ocular Barrier Penetration for Microinvasive Antiangiogenic Therapy

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: 147(9), P. 7545 - 7554

Published: Feb. 20, 2025

Therapeutic aptamers targeting vascular endothelial growth factor A (VEGFA) have advanced the development of antiangiogenic drugs for treating choroidal neovascularization (CNV) diseases. However, despite FDA approval use in neovascular age-related macular degeneration (nAMD), effective vivo delivery therapeutic is hindered by ocular barriers and rapid degradation biofluids. Here, we demonstrated a microinvasive VEGFA-targeted to fundus using tetrahedral framework nucleic acids (tFNAs). Upon incorporating anti-VEGFA tFNAs (apt-tFNA), interrogated their penetration across outer blood-retinal barrier (oBRB) innermost retinal eyeball, while maintaining structural integrity. In addition, apt-tFNA showed superior efficacy inhibiting proliferation migration neutralizing VEGFA. Furthermore, laser-induced CNV mouse model, subconjunctival injection exhibited comparable intravitreal ranibizumab, monoclonal antibody fragment. These findings suggest that FNAs can effectively deliver without compromising properties, highlighting potential feasible periocular administration ophthalmic

Language: Английский

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MoS₂-DNA tetrahedral bioconjugate for high-performance DNA biosensors: application in viral infection diagnostics

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(4)

Published: March 11, 2025

Abstract An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral nanostructures (TDNs), and thionine-modified carbon nanodots (CNDsTy). The innovation of this work lies in the first-time integration these nanomaterials preparation a bioconjugate, whose synergy enables biosensor’s functionality. MoS₂ anchors TDNs, which carry capture probe virus identification via genetic code recognition. CNDsTy allow detection based on their different affinity single-stranded (ssDNA) double-stranded (dsDNA), enabling hybridization event identification. achieves high sensitivity (detection limit 5.00 fM) can distinguish loads, validated with SARS-CoV-2 ORF1ab sequence human nasopharyngeal samples. Graphical

Language: Английский

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Neutrophil Extracellular Traps: Emerging Biomarker and Prototype of Functional Materials

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

Abstract Functional biomaterials composed of multiple biomacromolecules have significant advantages over those made from a single type. However, harmoniously integrating various remains challenging. Neutrophil extracellular traps (NETs), an emerging biological structure released neutrophils, serve as natural prototype worth investigating and learning from. NETs consist intricate exhibit web‐like microstructure, endowing them with multifaceted roles in both physiological pathological processes. In this review, research progress is systematically examined on materials science perspective. First, the origin transformation are introduced, their functional mechanisms NET components thoroughly dissected rather than conventionally treating entity. Second, given complex functions, revealed potential biomarker for disease prediction. Third, typical characterization technologies analysis summarized. Fourth, artificial inspired by discussed. addition, regulatory processes provide bio‐inspired prototypes design advanced materials. Finally, perspectives opportunities challenges advancing presented biomarkers models development

Language: Английский

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Programmable AIESTA: All-in-One Isothermal Enzymatic Signal Transduction Amplifier for Portable Profiling

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

The Argonaute (Ago) protein exhibits high specificity in nucleic acid recognition and cleavage, making it highly promising for biosensing applications. Its potential is further enhanced by its independence from protospacer adjacent motif (PAM) requirements the cost-effectiveness of using short DNA guides. Both Ago CRISPR/Cas systems face challenges signal amplification, which limit their ability to detect targets at ultralow concentrations. To overcome this limitation, a thermostable quadratic amplification system (T-QAS) was constructed integrating nicking-enzyme-mediated (NEMA) strategy with TtAgo. leverages stability T-QAS elevated temperatures enhance guide-target interactions decrease false positives caused nonspecific amplification. Additionally, nanozyme integrated construct AIESTA platform (all-in-one isothermal enzymatic transduction amplifier), single-tube visual sensing platform. Within system, improves through operational offers programmable functions, enabling sensitive detection miRNA foodborne toxins. combination makes candidate point-of-care testing (POCT) field, showcasing resource-limited complex environments.

Language: Английский

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Smart nucleic acid nanodrug delivery system for precision therapeutics

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 535, P. 216673 - 216673

Published: April 3, 2025

Language: Английский

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