BMC Chemistry,
Journal Year:
2025,
Volume and Issue:
19(1)
Published: March 8, 2025
Abstract
Straightforward,
eco-friendly,
quick,
and
sensitive
spectrophotometric
procedures
were
created
proven
to
be
effective
for
determining
the
amount
of
duloxetine
amitriptyline
in
bulk
pharmaceutical
dosage
forms.
The
basis
suggested
was
formation
an
ion
association
complex
aqueous
buffered
solution
containing
with
eosin
Y.
resulting
compound
displayed
absorption
peaks
at
546
nm
under
optimum
circumstances.
With
a
linear
relationship
good
correlation
value
0.9996
DLX
0.9997
AMT,
calibration
plots
rectilinear
over
concentration
range
0.5–8
µg
mL
−
1
1–7
AMT.
quantitation
limits
0.48
0.49
respectively,
whereas
detection
0.16
both
drugs.
research
process
has
been
optimized
respect
many
experimental
parameters.
approaches
assessed
accordance
ICH
guidelines.
successfully
used
analyze
formulations,
including
cited
medications.
Additionally,
recommended
methods
performed
admirably
when
assess
content
uniformity.
proposed
method
is
highly
green
as
water
solvent.
Utilizing
four
metric
tools
called
NEMI,
Eco-Scale,
GAPI,
AGREE,
established
techniques’
environmental
impact
assessed.
Also,
practicality
(blueness)
using
recently
developed
Blue
Applicability
Grade
Index
(BAGI).
BMC Chemistry,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: May 3, 2024
Abstract
The
simultaneous
assay
of
duloxetine
hydrochloride
(DLX)
and
avanafil
(AVN)
in
their
pure
forms,
synthetic
mixtures,
spiked
human
plasma
was
achieved
using
a
novel,
eco-friendly,
sensitive,
specific
HPTLC
methodology
that
have
been
established
validated.
Measuring
the
levels
co-administered
antidepressants
sexual
stimulants
biological
fluids
is
an
important
step
for
individuals
with
depression
problems.
Separation
performed
successfully
pre-coated
silica
gel
60-F254
as
stationary
phase
mobile
composed
methanol,
acetone,
33%
ammonia
(8:2:0.05,
v/v/v).
Compact
bands
were
produced
by
optimized
chosen
development
(Rf
values
0.23
0.75
DLX
AVN,
individually)
after
dual-wavelength
detection
AVN
at
232
253
nm,
respectively.
results
polynomial
regression
analysis
exceptional
(
r
=
0.9999
both
medicines)
over
concentration
ranges
5-800
10-800ng/spot
quantitation
limits
4.69
9.53
ng/spot
(0.31
0.94
µg/mL),
whereas
1.55
3.15
(0.63
1.91
International
Council
Harmonization
(ICH)
criteria
served
basis
validating
approach.
Moreover,
proposed
technique
evaluated
terms
greenness
four
contemporary
ecological
metrics:
Analytical
Greenness
software
(AGREE),
Green
Procedure
Index
(GAPI),
Eco-Scale,
National
Environmental
Method
(NEMI).
Additionally,
Blue
Applicability
Grade
(BAGI),
newly
developed
tool
evaluating
practicality
(blueness)
procedures,
taken
into
consideration
when
sustainability
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Oct. 13, 2024
Abstract
A
novel,
environmentally
friendly,
and
sensitive
HPTLC
method
has
been
developed
validated
for
simultaneous
quantification
of
duloxetine
(DLX)
tadalafil
(TDL)
in
their
pure
state,
laboratory-prepared
mixtures,
spiked
human
plasma.
This
is
particularly
important
patients
dealing
with
depression
sexual
issues,
as
it
allows
the
measurement
these
co-administered
antidepressant
stimulant
drugs
biological
fluids.
The
separation
process
employed
a
stationary
phase
pre-coated
silica
gel
60
F
254
mobile
consisting
ethyl
acetate,
acetonitrile,
33%
ammonia
(8:1:1,
v/v).
optimized
resulted
well-defined
bands
DLX
TDL,
Rf
values
0.3
0.8,
respectively
dual-wavelength
detection
at
232
nm
222
TDL.
Polynomial
regression
analysis
revealed
exceptional
linearity
both
drugs,
correlation
coefficients
0.9999
over
concentration
ranges
10–900
ng/band
10-1200
quantitation
limits
were
8.2
8.6
while
(LOD)
2.7
2.8
validation
this
followed
guidelines
set
by
International
Council
Harmonization
(ICH).
Additionally,
suggested
method’s
greenness
was
assessed
means
four
up-to-date
ecological
tools,
namely
Eco-Scale,
National
Environmental
Method
Index
(NEMI),
Green
Analytical
Procedure
(GAPI),
Greenness
metric
approach
(AGREE).
proposed
also
using
Blue
Applicability
Grade
(BAGI),
recently
assessing
practicality
(blueness)
procedures.
Luminescence,
Journal Year:
2025,
Volume and Issue:
40(1)
Published: Jan. 1, 2025
ABSTRACT
This
study
introduces
a
novel
synchronous
spectrofluorimetry
coupled
with
chemometric
tools
for
the
determination
of
tenofovir
and
dolutegravir
antiretroviral
drugs.
Utilizing
partial
least
squares
regression
(PLS)
fine‐tuned
by
genetic
algorithm
as
variable
selection
tool,
developed
models
demonstrate
greater
sensitivity,
cost‐effectiveness,
reduced
environmental
impact
compared
to
traditional
HPLC
methods.
The
model's
validation
was
further
confirmed
using
external
in
addition
QC
samples
per
ICH
M10
guidelines,
which
yielded
high
accuracy
ranged
between
94.78%
102.48%
94.56%
103.31%
dolutegravir,
respectively.
Additionally,
%CV
values
within‐run
precision
were
below
4%,
while
those
between‐run
stayed
under
6%,
Hence,
adeptly
applied
pharmacokinetic
studies
rat
model,
revealing
slight
changes
plasma
concentration
profiles
half‐lives
when
administered
combination,
highlighting
possible
drug
interactions.
Greenness
analysis
this
spectrofluorimetric
approach
AGREE
tool
substantiates
its
advantages
over
conventional
chromatographic
techniques.
simple,
rapid,
environmentally
friendly
analytical
methodology
presents
promising
alternative
therapeutic
monitoring
BMC Chemistry,
Journal Year:
2025,
Volume and Issue:
19(1)
Published: April 2, 2025
Abstract
Antidepressant
drugs,
particularly
selective
serotonin
reuptake
inhibitors
(SSRIs)
and
serotonin-norepinephrine
(SNRIs)
are
known
to
induce
sexual
dysfunction
as
a
side
effect.
Duloxetine
(DLX)
Avanafil
(AVN)
simultaneously
determined
in
their
pure
state
laboratory-prepared
mixtures
by
two
novel,
environmentally
friendly
spectrophotometric
methods.
The
first
method
was
based
on
second
order
derivatives
while
the
is
derivative
dual-wavelength.
In
I,
linearity
range
found
be
0.5–12
µg/mL
1–12
with
limit
of
detection
0.15
0.27
for
DLX
AVN,
respectively.
II,
1–10
both
drugs
0.18
0.21
validation
these
approaches
meets
International
Council
Harmonization’s
(ICH)
standards.
Furthermore,
three
current
ecological
tools
namely
Eco-Scale,
GAPI,
AGREE
were
used
evaluate
proposed
method’s
greenness.
sustainability
characteristics
also
assessed
using
Blue
Applicability
Grade
Index
(BAGI),
recently
developed
metric
assessing
practicality
(blueness)
procedures.
Luminescence,
Journal Year:
2025,
Volume and Issue:
40(5)
Published: May 1, 2025
ABSTRACT
In
this
study,
we
present
a
direct,
sensitive,
and
green
spectrofluorimetric
approach
for
simultaneous
measurement
of
bisoprolol
fumarate
(BSL)
amlodipine
besylate
(AMD)
in
their
tablets
plasma.
This
measures
the
synchronized
fluorescence
intensity
two
drugs
at
Δ
λ
55
nm
with
max
captured
265
375
BSL
AMD,
respectively.
The
method
is
linear
over
concentration
range
0.1–4.1
μg/mL
1.0–9.0
AMD.
limit
detection
AMD
0.0168
0.1227,
quantification
0.0508
0.3717
μg/mL,
technique
effectively
used
to
analyze
studied
high
%Recoveries
100.09%
99.67%
%RSD
≤
1.54.
proposed
verified
compliance
ICH
Q2(R2)
criteria.
Hence,
suggested
assesses
content
uniformity
accordance
British
Pharmacopoeia.
Besides,
determined
plasma
0.1–0.5
percentage
recoveries
(98.95%–101.60%)
low
values
(1.326).
greenness
developed
was
positively
assessed
using
three
distinct
metrics,
including
AGREE,
GAPI,
BAGI.
