3D bioprinting using a new photo-crosslinking method for muscle tissue restoration

npj Regenerative Medicine, Journal Year: 2023, Volume and Issue: 8(1)

Published: March 31, 2023

Three-dimensional (3D) bioprinting is a highly effective technique for fabricating cell-loaded constructs in tissue engineering. However, the versatility of precise and complex hydrogels limited owing to poor crosslinking ability cell-containing hydrogels. Herein, we propose an optic-fiber-assisted (OAB) process efficiently crosslink methacrylated By selecting appropriate processing conditions photo-crosslinking technique, fabricated biofunctional cell-laden structures including gelatin (Gelma), collagen, decellularized extracellular matrix. To apply method skeletal muscle regeneration, Gelma were processed with functional nozzle having topographical cue OAB that could induce uniaxial alignment C2C12 human adipose stem cells (hASCs). Significantly higher degrees cell myogenic activities structure observed compared those construct was printed using conventional method. Moreover, vivo regenerative potential volumetric defects mouse model. The hASC-laden significantly induced greater regeneration than without cues. Based on results, newly designed can prove be various engineering applications.

Language: Английский

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Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction

Science Advances, Journal Year: 2024, Volume and Issue: 10(17)

Published: April 24, 2024

Myotendinous junction (MTJ) injuries are prevalent in clinical practice, yet the treatment approaches limited to surgical suturing and conservative therapy, exhibiting a high recurrence rate. Current research on MTJ tissue engineering is scarce lacks vivo evaluation of repair efficacy. Here, we developed three-dimensional-printed bioactive fiber-reinforced hydrogel containing mesenchymal stem cells (MSCs) Klotho for structural functional regeneration. In rat defect model, promoted restoration muscle, tendon, muscle-tendon interface enhanced recovery injured MTJ. proteomics vitro cell cultures elucidated regenerative mechanisms by modulating oxidative stress inflammation, thus an optimized microenvironment support survival differentiation transplanted MSCs maintain phenotype resident within tissues, including tendon/muscle macrophages. This strategy provides promising injuries.

Language: Английский

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Hierarchy Reproduction: Multiphasic Strategies for Tendon/ligament-bone Junctions Repair

Biomaterials Research, Journal Year: 2025, Volume and Issue: 29

Published: Jan. 1, 2025

Tendon/ligament-bone junctions (T/LBJs) are susceptible to damage during exercise, resulting in anterior cruciate ligament rupture or rotator cuff tear; however, their intricate hierarchical structure hinders self-regeneration. Multiphasic strategies have been explored fuel heterogeneous tissue regeneration and integration. This review summarizes current multiphasic approaches for rejuvenating functional gradients T/LBJ healing. Synthetic, natural, organism-derived materials available vivo validation. Both discrete gradient layouts serve as sources of inspiration organizing specific cues, based on the theories biomaterial topology, biochemistry, mechanobiology, situ delivery therapy, which form interconnected network within design. Novel engineering can be constructed by electrospinning, 3-dimensional printing, bioprinting, textiling, other techniques. Despite these efforts being limited at present stage, scaffolds show great potential precise reproduction native T/LBJs offer promising solutions clinical dilemmas.

Language: Английский

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3D printing in musculoskeletal interface engineering: Current progress and future directions

Advanced Drug Delivery Reviews, Journal Year: 2025, Volume and Issue: 219, P. 115552 - 115552

Published: March 1, 2025

Language: Английский

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Tumor-on-a-chip models combined with mini-tissues or organoids for engineering tumor tissues

Theranostics, Journal Year: 2023, Volume and Issue: 14(1), P. 33 - 55

Published: Oct. 30, 2023

The integration of tumor-on-a-chip technology with mini-tissues or organoids has emerged as a powerful approach in cancer research and drug development. This review provides an extensive examination the diverse biofabrication methods employed to create mini-tissues, including 3D bioprinting, spheroids, microfluidic systems, self-assembly techniques using cell-laden hydrogels. Furthermore, it explores various approaches for fabricating organ-on-a-chip platforms. paper highlights synergistic potential combining these technologies models that mimic complex tumor microenvironment offer unique insights into biology therapeutic responses.

Language: Английский

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Nanomaterials-Based Hybrid Bioink Platforms in Advancing 3D Bioprinting Technologies for Regenerative Medicine

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(7), P. 4145 - 4174

Published: June 1, 2024

3D bioprinting is recognized as the ultimate additive biomanufacturing technology in tissue engineering and regeneration, augmented with intelligent bioinks bioprinters to construct tissues or organs, thereby eliminating stipulation for artificial organs. For of soft tissues, such kidneys, hearts, other human body parts, formulations bioink enhanced bioinspired rheological mechanical properties were essential. Nanomaterials-based hybrid have potential overcome above-mentioned problem require much attention among researchers. Natural synthetic nanomaterials carbon nanotubes, graphene oxides, titanium nanosilicates, nanoclay, nanocellulose, etc. their blended been used various benefitted bioprintability, biocompatibility, biodegradability. A limited number articles published, requirement pushed us write this review. We reviewed, explored, discussed nanocomposite-based technology, properties, natural, synthetic, nanomaterial-based bioinks, including applications challenges, limitations, ethical considerations, solution future perspective, technological advancement efficient cost-effective methods regeneration healthcare.

Language: Английский

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A bioprinted complex tissue model for myotendinous junction with biochemical and biophysical cues

Bioengineering & Translational Medicine, Journal Year: 2022, Volume and Issue: 7(3)

Published: April 4, 2022

In the musculoskeletal system, myotendinous junction (MTJ) is optimally designed from aspect of force transmission generated a muscle through tendon onto bone to induce movement. Although MTJ key complex tissue in transmission, realistic fabrication, and formation tissues can be limited. To obtain construct, we prepared two bioinks, muscle- tendon-derived decellularized extracellular matrix (dECM), which myogenic tenogenic differentiation human adipose-derived stem cells (hASCs). By using modified bioprinting process supplemented with nozzle consisting single-core channel double-sheath channels, achieve three different types units, composed muscle, tendon, interface zones. Our results indicated that bioprinted dECM-based constructs induced hASCs differentiation. addition, significantly higher MTJ-associated gene expression was detected at cell-mixing zone than other models. Based on results, model potential platform for understanding interaction between cells, even method extensively applied tissues.

Language: Английский

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Electrospun Aligned Nanofiber Yarns Constructed Biomimetic M‐Type Interface Integrated into Precise Co‐Culture System as Muscle‐Tendon Junction‐on‐a‐Chip for Drug Development

Small Methods, Journal Year: 2024, Volume and Issue: 8(9)

Published: April 9, 2024

Abstract The incorporation of engineered muscle‐tendon junction (MTJ) with organ‐on‐a‐chip technology provides promising in vitro models for the understanding cell‐cell interaction at interface between muscle and tendon tissues. However, developing MTJ tissue biomimetic anatomical structure remains challenging, precise co‐culture is further regarded as a remarkable obstacle. Herein, an interwoven waving approach presented to develop “M‐type” structure, integrated into microfluidic device functional MTJ‐on‐a‐chip fabrication. These multiscale scaffolds based on electrospun nanofiber yarns enabled 3D cellular alignment differentiation, led organization zone. Crucially, compartmentalized system zones using their medium same time. Such served drug‐associated toxic or protective efficacy investigations. results highlight that these nanofibrous are beneficial development, indicated potential musculoskeletal drug development biological mechanism studies.

Language: Английский

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Integrated design and fabrication strategies based on bioprinting for skeletal muscle regeneration: Current status and future perspectives

Materials & Design, Journal Year: 2023, Volume and Issue: 225, P. 111591 - 111591

Published: Jan. 1, 2023

The number of skeletal muscle injuries derived from myopathies, exercise, and trauma, is growing due to increasing sports activities in normal life people. Skeletal has a robust capacity for regeneration following injury. However, few if any effective therapeutic options volumetric loss are available. Poor repair injury will lead dysfunction, resulting physical pain even seriously compromising daily life. As new technology biomedicine, 3D bioprinting widely used the field tissue engineering regenerative medicine its enormous advantages. creates biological structures similar that protists by precisely constructing specified geometric shape at micro/nano levels, thus addressing unmet needs replacement organ transplantation. With further application various repairs, more researchers utilized regeneration. Various bioinks, such as alginate, gelatin, fibrin hydrogels, extracellular matrix (ECM), loaded with functional seed cells or growth factors have been applied fabricating bioprinted constructs complicated inner Therefore, we conducted this review report process, development status technology, mechanism promoting repair. In addition, current challenges perspectives also discussed study.

Language: Английский

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Advances focusing on the application of decellularization methods in tendon-bone healing

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The tendon or ligament is attached to the bone by a triphasic but continuous area of heterogeneous tissue called tendon-bone interface (TBI). rapid and functional regeneration TBI challenging owing its complex composition difficulty in self-healing. development new technologies, such as decellularization, has shown promise TBI. Several ex vivo studies have that decellularized grafts biomaterial scaffolds achieved better efficacy enhancing healing. However further information on type review available needed. In this review, we discuss current application decellularization promoting healing possible mechanisms involved. With work, would like reveal how tissues biomaterials been can improve provide theoretical basis for future related studies. Decellularization an emerging technology utilizes various chemical, enzymatic and/or physical strategies remove cellular components from while retaining structure extracellular matrix (ECM). After cause immune response are removed, biologically active biofactors retained. This explores

Language: Английский

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