Applications of plant‐derived extracellular vesicles in medicine DOI Creative Commons

Yawen Zhu,

Junqi Zhao, Haoran Ding

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 20, 2024

Plant-derived extracellular vesicles (EVs) are promising therapeutic agents owing to their natural abundance, accessibility, and unique biological properties. This review provides a comprehensive exploration of the potential plant-derived EVs emphasizes anti-inflammatory, antimicrobial, tumor-inhibitory effects. Here, we discussed advancements in isolation purification techniques, such as ultracentrifugation size-exclusion chromatography, which critical for maintaining functional integrity these nanovesicles. Next, investigated diverse administration routes carefully weighed respective advantages challenges related bioavailability patient compliance. Moreover, elucidated multifaceted mechanisms action EVs, including roles anti-inflammation, antioxidation, antitumor activity, modulation gut microbiota. We also impact on specific diseases cancer inflammatory bowel disease, highlighting importance addressing current production scalability, regulatory compliance, immunogenicity. Finally, proposed future research directions optimizing EV extraction developing targeted delivery systems. Through efforts, envision seamless integration into mainstream medicine, offering safe potent alternatives across various medical disciplines.

Language: Английский

A Comprehensive Review on Deep Eutectic Solvents: Their Current Status and Potential for Extracting Active Compounds from Adaptogenic Plants DOI Creative Commons
Małgorzata Stanisz, Beata Stanisz, Judyta Cielecka‐Piontek

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4767 - 4767

Published: Oct. 9, 2024

Deep eutectic solvents (DESs) have attracted attention from researchers as novel compounds for extracting active substances because of their negligible toxicity, polarity, and ability to be tailored depending on the experiment. In this review, we discuss deep a promising medium extraction adaptogenic compounds. comparison traditional methods, with use DESs is great alternative excessive usage harmful organic solvents. It can conducted in mild conditions, designed different precursors, enhancing versatility. Adaptogenic herbs long medicinal history, especially Eastern Asia. They exhibit unique properties through structures, including saponins, flavonoids, polysaccharides, alkaloids. Therefore, they demonstrate wide range pharmaceutical effects, such anti-inflammatory, antibacterial, anticancer abilities. Since ancient times, many been discovered are well known,

Language: Английский

Citations

4
Recent Advances in the Isolation Strategies of Plant-Derived Exosomes and Their Therapeutic Applications DOI Creative Commons

Jeong‐Geon Mun,

Dandan Song, Ji‐Ye Kee

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 144 - 144

Published: Feb. 22, 2025

Exosome-like nanovesicles (ELNs) derived from natural products are gaining attention as innovative therapeutic agents due to their biocompatibility, low immunogenicity, and capability transport bioactive molecules such proteins, lipids, nucleic acids. These plant-derived ELNs exhibit structural similarities with mammalian exosomes, making them suitable for drug delivery, microbiome-targeted therapies, regenerative medicine. Recent studies highlight potential in treating cancer, inflammation, metabolic disorders. Additionally, have applications cosmetics, agriculture, the food industry. This review combines latest advancements research on ELNs, focusing isolation techniques, pharmacological effects, applications. Although offer promising opportunities, several challenges must be addressed, including standardization, large-scale production, vivo efficacy. By summarizing cutting-edge suggesting future directions, we aim inspire further development of next-generation platforms.

Language: Английский

Citations

0
Bioprinted Hydrogels as Vehicles for the Application of Extracellular Vesicles in Regenerative Medicine DOI Creative Commons
Marta Camacho-Cardeñosa, Victoria Pulido-Escribano, Guadalupe Estrella-Guisado

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(3), P. 191 - 191

Published: March 8, 2025

Three-dimensional bioprinting is a new advance in tissue engineering and regenerative medicine. Bioprinting allows manufacturing three-dimensional (3D) structures that mimic tissues or organs. The bioinks used are mainly made of natural synthetic polymers must be biocompatible, printable, biodegradable. These may incorporate progenitor cells, favoring graft implantation regeneration injured tissues. However, the natures biomaterials, processes, lack vascularization, immune responses factors limit viability functionality implanted cells damaged limitations can addressed by incorporating extracellular vesicles (EV) into bioinks. Indeed, EV from have capacities, being similar to those their source cells. Therefore, combinations with biomaterials cell-free therapies. Likewise, they complement manufacture increasing viability, differentiation, ability incorporated Thus, main objective this review show how use 3D technology for application medicine these nanovesicles hydrogels as To end, latest advances derived vitro vivo studies been described. Together, high therapeutic potential strategy

Language: Английский

Citations

0
Advances in plant-derived vesicle like nanoparticles-based therapies for inflammatory diseases DOI Creative Commons
Zhifei Cheng, Wenbo Lu, Wei Shao

et al.

Asian Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 101052 - 101052

Published: April 1, 2025

Language: Английский

Citations

0
Pomegranate‐Derived Exosome‐Like Nanovesicles Containing Ellagic Acid Alleviate Gut Leakage and Liver Injury in MASLD DOI Creative Commons
Jisu Kim, Byoung‐Joon Song,

Young‐Eun Cho

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: April 1, 2025

ABSTRACT Exosome‐like nanovesicles derived from plants (PENs) harbor a spectrum of bioactive compounds, including proteins, lipids, nucleic acids (such as miRNAs and mRNAs), offering therapeutic advantages for variety diseases. This investigation assesses the utility pomegranate‐derived exosome‐like (PNVs) in both preventing treating complications such liver damage increased intestinal permeability found Metabolic Dysfunction‐Associated Steatotic Liver Disease (MASLD). Utilizing Transmission Electron Microscopy (TEM) Nanoparticle Tracking Analysis (NTA), we successfully isolated PNVs characterized their structural properties. Upon administration live model, these were efficiently distributed to critical organs, intestines, demonstrating biocompatibility by avoiding toxic effects. Remarkably, vesicles enriched with ellagic acid, known its strong antioxidant capabilities. In controlled MASLD mouse study, treatment significantly lowered serum endotoxin levels, reinforced barrier functions, altered gut microbiota profile favorably. Furthermore, application reduced oxidative stress presence fibrosis markers normal levels. These results indicate that could be viable option addressing MASLD, enhancing integrity, reducing injury, diminishing through modulation gut‐liver axis.

Language: Английский

Citations

0
Stimuli-Responsive Nanomedicines for the Treatment of Non-cancer Related Inflammatory Diseases DOI Creative Commons
Jingjing Yang, Anne des Rieux, Alessio Malfanti

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Nanomedicines offer a means to overcome the limitations associated with traditional drug dosage formulations by affording protection, enhanced bioavailability, and targeted delivery affected sites. Inflamed tissues possess unique microenvironmental characteristics (including excessive reactive oxygen species, low pH levels, hypoxia) that stimuli-responsive nanoparticles can employ as triggers support on-demand delivery, accumulation, controlled release, activation of anti-inflammatory drugs. Stimuli-responsive nanomedicines respond physicochemical pathological factors diseased improve specificity multidrug resistance, ensure accurate diagnosis precision therapy, control release efficacy safety. Current react intracellular/microenvironmental stimuli such pH, redox, hypoxia, or specific enzymes exogenous temperature, magnetic fields, light, ultrasound via bioresponsive moieties. This review summarizes general strategies employed produce tailored for inflammatory diseases all recent advances, reports their applications in illustrates progress made toward clinical translation.

Language: Английский

Citations

0
Harnessing exosomes and plant-derived exosomes as nanocarriers for the efficient delivery of plant bioactives DOI
Pooja Kathait, Pradeep Kumar Patel, Alakh N. Sahu

et al.

Nanomedicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: June 20, 2024

Exosomes, a category of extracellular vesicle (EV), are phospholipid bilayer structures ranging from 30 to 150 nm, produced by various organisms through the endosomal pathway. Recent studies have established utilization exosomes as nanocarriers for drug distribution across therapeutic areas including cancer, acute liver injury, neuroprotection, oxidative stress, inflammation, etc. The importance plant-derived and exosome vesicles derived mammalian cells or milk, loaded with potent plant bioactives indications discussed along insights into future perspectives. Moreover, this review provides detailed understanding biogenesis, their composition, classification, stability different types exosomes, routes administration standard techniques used isolating, purifying, characterizing exosomes.

Language: Английский

Citations

3
Innovative Strategies in 3D Bioprinting for Spinal Cord Injury Repair DOI Open Access

Daniel Youngsuk Kim,

Yanting Liu,

Gyubin Kim

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9592 - 9592

Published: Sept. 4, 2024

Spinal cord injury (SCI) is a catastrophic condition that disrupts neurons within the spinal cord, leading to severe motor and sensory deficits. While current treatments can alleviate pain, they do not promote neural regeneration or functional recovery. Three-dimensional (3D) bioprinting offers promising solutions for SCI repair by enabling creation of complex tissue constructs. This review provides comprehensive overview 3D techniques, bioinks, stem cell applications in repair. Additionally, it highlights recent advancements bioprinted scaffolds, including integration conductive materials, incorporation bioactive molecules like neurotrophic factors, drugs, exosomes, design innovative structures such as multi-channel axial scaffolds. These strategies offer approach optimizing microenvironment, advancing understanding state repair, offering insights into future directions field regenerative medicine.

Language: Английский

Citations

3
Applications of plant‐derived extracellular vesicles in medicine DOI Creative Commons

Yawen Zhu,

Junqi Zhao, Haoran Ding

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 20, 2024

Plant-derived extracellular vesicles (EVs) are promising therapeutic agents owing to their natural abundance, accessibility, and unique biological properties. This review provides a comprehensive exploration of the potential plant-derived EVs emphasizes anti-inflammatory, antimicrobial, tumor-inhibitory effects. Here, we discussed advancements in isolation purification techniques, such as ultracentrifugation size-exclusion chromatography, which critical for maintaining functional integrity these nanovesicles. Next, investigated diverse administration routes carefully weighed respective advantages challenges related bioavailability patient compliance. Moreover, elucidated multifaceted mechanisms action EVs, including roles anti-inflammation, antioxidation, antitumor activity, modulation gut microbiota. We also impact on specific diseases cancer inflammatory bowel disease, highlighting importance addressing current production scalability, regulatory compliance, immunogenicity. Finally, proposed future research directions optimizing EV extraction developing targeted delivery systems. Through efforts, envision seamless integration into mainstream medicine, offering safe potent alternatives across various medical disciplines.

Language: Английский

Citations

1