Artificial Cell Wall: From Maintenance of Cell Viability to Boosting New Cellular Functionalities^†

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Comprehensive Summary Artificial cell wall (ACW) referring to active functional cellular nano‐coatings is capable of providing more cell‐shell synergic and cooperative properties than conventional single nanoencapsulation (SCNE). With the development SCNE, issues cytocompatibility, degradability, etc ., have already been improved successively. However, further emphasis on cooperativity between itself its shell still missing paying attention functions hybrids. Recent research proved that construction nano‐coating cells not only needs satisfy functionalization cells, but also necessary empower interact with other environments. This indicates SCNEs are tending be “active” participate in metabolic process gradually develop stage ACWs. review provided a reasonable description artificial wall, realization this concept requires cooperativity, self‐adaption fluxionality. Then, methodologies constructing ACWs were discussed. Finally, applications summarized accompanied by potential outlook given fields. Key Scientists Within recent ten years, fabricating biocompatible surface for cyto‐protection rapidly developed. For example, Rawil F. Fakhrullin Yuri M. Lvov developed ‘layer‐by‐layer’ strategy construct ‘face‐lifting’ micro‐organisms. Afterwards, Choi introduced ‘artificial spore’ which thin tough encapsulations. At same time, Tsukruk hydrogel‐based terms 2013. In 2016, Tang reported biomimetic mineralization can used improve modify metabolism processes cells. More advanced, Qu Hawker separately way introduce manganese dioxide nanozymes in‐situ ‐grafting polymers realize responsive effects towards regulation 2017. 2018, Huang showed generate type heritable based situ self‐assembly coacervate micro‐droplets around yeast 2019, Jeffrey nanoparticles adopted exoskeletons outside endow new forming ‘supracells’. put forward concepts ‘active shell’ ‘dynamic indicated nanoencapsulations was beginning transition control fate degradable walls 2020 an intracellular method regulate algae promote hydrogen production. Based his previous research, focused introducing fabrication wall’.

Language: Английский

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Mechanism and improvement of yeast tolerance to biomass-derived inhibitors: A review

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108562 - 108562

Published: March 1, 2025

Language: Английский

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Production of yeast cell wall polysaccharides‐β‐glucan and chitin by using food waste substrates: Biosynthesis, production, extraction, and purification methods

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(3)

Published: April 4, 2025

Food waste causes significant environmental and economic challenges worldwide, prompting many nations to prioritize its reduction recycling. As a nutrient-rich material containing vitamins, proteins, carbohydrates, it serves as promising substrate for the cultivation of single-cell microorganisms like yeast. Yeast cell wall polysaccharides (YCWPs), particularly chitin β-glucans, offer valuable applications in food, pharmaceuticals, bioprocessing. This review highlights biosynthesis, production, extraction, purification YCWP cultivated on food substrates. Key species including Saccharomyces cerevisiae, Pichia pastoris, Candida spp. are discussed, with focus optimizing β-glucan yield through mechanical, chemical, enzymatic extraction methods. In addition, structural functional properties β-glucans maintaining stability explored, emphasizing their potential prebiotics, dietary fibers, biodegradable packaging materials. also examines valorization yeast cultivation, presenting sustainable bioprocessing strategy transforming into bioproducts.

Language: Английский

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Recovery of Sustainable Yeast Proteins: A Mechanistic Study of Autolysis and Enzyme Proteolysis as Pretreatments for Mechanical Cell Rupture

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Single-cell protein from yeast can provide a sustainable means to meet increasing demands. For be digestible it must released within the cells using methods such as autolysis, enzyme proteolysis, and mechanical rupture. This study investigates combination of autolysis or proteolysis subsequent rupture on release. The effects papain size, wall thickness, internal structure freshly harvested 7 day stored Saccharomyces cerevisiae were investigated particle sizing electron microscopy. Samples then subjected high-pressure homogenization (single pass, 800 bar) extent cell solubilization determined. During intracellular contents released, shrinking cells, allowing elastic walls contract thicken. Autolyzed mostly deformed rather than ruptured by homogenization, which expelled contents. Intact untreated with thin, stretched burst during proteins remaining unsolubilized. Stored smaller thicker generally more difficult These findings assist in developing efficient processes for recovering sustainably produced other microorganisms.

Language: Английский

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Yeast polysaccharides: The environmentally friendly polysaccharides with broad application potentials

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(5)

Published: Sept. 1, 2024

Abstract Yeast cell wall (YCW) polysaccharides, including β‐glucans, mannans, chitins, and glycogens, can be extracted from the waste of beer industry. They are environmentally friendly, abundant, inexpensive raw materials, have shown broad biological activities application potentials. The exploitation yeast polysaccharides is great importance for environmental protection resource utilization. This paper reviews structural features preparation YCW polysaccharides. solubility emulsification properties binding metal ions presented. In addition, such as blood glucose lipid lowering, immune regulation, antioxidant, promotion intestinal health, wound healing proposed, highlighting beneficial effects on human health. Through modification, physical chemical changed, which emphasizes their properties. food applications packaging film, emulsifier, thickening agent, fat alternatives, focused discussed.

Language: Английский

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Prospects for the use of complex additive to improve biotechnological characteristics of yeast Saccharomyces Cerevisiae

Proceedings of the Voronezh State University of Engineering Technologies, Journal Year: 2024, Volume and Issue: 86(3), P. 96 - 101

Published: Sept. 25, 2024

One of the main factors affecting quality bread are microbiological and biochemical processes occurring in dough semifinished products as a result yeast activity. These can be controlled by regulating biotechnological characteristics yeast, which allows to intensify technological operations baking production. Baking should have high enzymatic activity, ability release carbon dioxide anaerobic conditions quickly adapt changes composition nutrient medium. properties improved pre-activation media containing components that synthesis fermentation enzymes cells. The aim work was study effect pressed baker's Saccharomyces cerevisiae with food complex additive on their characteristics: lifting force activity (zymase, maltase). Yeast determined accelerated method. Zymase maltase activities were measuring time for 10 cm3 is released during digestion glucose maltose solutions respectively. novelty consists establishment parameters preliminary activation serevisiae use additive. It shown optimal duration 20 min. Pre-activation contributed increase increased 46.9 %, zymase - 37.7 13.4 %. obtained results indicate promising application medium production bakery

Language: Английский

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