Photobiocatalytic Strategies for Organic Synthesis

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(9), P. 5459 - 5520

Published: April 28, 2023

Biocatalysis has revolutionized chemical synthesis, providing sustainable methods for preparing various organic molecules. In enzyme-mediated most reactions involve molecules operating from their ground states. Over the past 25 years, there been an increased interest in enzymatic processes that utilize electronically excited states accessed through photoexcitation. These photobiocatalytic a diverse array of reaction mechanisms are complementary to one another. This comprehensive review will describe state-of-the-art strategies photobiocatalysis synthesis until December 2022. Apart reviewing relevant literature, central goal this is delineate mechanistic differences between general employed field. We organize based on relationship photochemical step and transformations. The include studies, substrate scopes, protein optimization strategies. By clearly defining mechanistically-distinct chemistry, we hope illuminate future synthetic opportunities area.

Language: Английский

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Biocatalysis for the Synthesis of Active Pharmaceutical Ingredients in Deep Eutectic Solvents: State-of-the-Art and Prospects

Catalysts, Journal Year: 2024, Volume and Issue: 14(1), P. 84 - 84

Published: Jan. 19, 2024

Biocatalysis holds immense potential for pharmaceutical development as it enables synthetic routes to various chiral building blocks with unparalleled selectivity. Therein, solvent and water use account a large contribution the environmental impact of reactions. In spirit Green Chemistry, transition from traditional highly diluted aqueous systems intensified non-aqueous media overcome limitations (e.g., shortages, recalcitrant wastewater treatments, low substrate loadings) has been observed. Benefiting spectacular advances in enzyme stabilization techniques, plethora biotransformations non-conventional have established. Deep eutectic solvents (DESs) emerge sort (potentially) greener medium increasing biocatalysis. This review discusses state-of-the-art DESs focus on biocatalytic pathways synthesis active ingredients (APIs). Representative examples different classes are discussed, together critical vision discussing prospects using

Language: Английский

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From green to circular chemistry paved by biocatalysis

Green Chemistry, Journal Year: 2023, Volume and Issue: 25(18), P. 7041 - 7057

Published: Jan. 1, 2023

Biocatalysts raised by the green chemistry and circular principles can constitute most important efficient strategy for achieving many of 17 Sustainable Development Goals launched UN.

Language: Английский

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The joint effort of enzyme technology and flow chemistry to bring biocatalytic processes to the next level of sustainability, efficiency and productivity

Journal of Flow Chemistry, Journal Year: 2023, Volume and Issue: 14(1), P. 85 - 96

Published: Nov. 7, 2023

Abstract The number of biocatalyzed reactions at industrial level is growing rapidly together with our understanding on how we can maximize the enzyme efficiency, stability and productivity. While biocatalysis nowadays recognized as a greener way to operate in chemistry, its combination continuous processes has lately come up powerful tool enhance process selectivity, productivity sustainability. This perspective aims describing recent advances this technology future developments leading smart, efficient strategies for optimization large-scale production. Graphical

Language: Английский

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Biocatalytic conversion of fatty acids into drop-in biofuels: Towards sustainable energy sources

EFB Bioeconomy Journal, Journal Year: 2023, Volume and Issue: 3, P. 100049 - 100049

Published: March 10, 2023

Intensifying effects of global warming necessitates swift solutions for decarbonization. A big source the increasing atmospheric CO 2 is burning transportation fuels. Although electrification light vehicles an important step to reduce emissions, carbon-based fuels will still be likely power up heavy-duty transport, marine and aviation some time. Thus, fastest way decarbonize these sectors could immediate transition carbon-neutral biofuels. Recently, conversion fatty acids into hydrocarbons, as "drop-in biofuels", emerging a promising alternative fossil However, current technologies rely on energy intensive chemical methods that are not quite eco-friendly. Therefore, enzymatic hydrocarbons present benign production method. In last decade, multiple enzymes have been discovered convert free alka(e)nes. Here, we introduce with description studies performed so far, especially regarding cell-free biocatalysis.

Language: Английский

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Design and Construct Cyclic Carbonate Functionalized Chitosan‐Based Hydrogel Enabling Photodecarboxylase Immobilization with Enhanced Stability and Reusability

Advanced Sustainable Systems, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 26, 2025

Abstract Fatty acid photodecarboxylase (FAP) plays a crucial role in the green production of biofuel and other valuable biochemicals. However, reusability immobilized FAP has been limited due to inadequate durability. Here, porous, translucent chitosan hydrogel sphere carrier functionalized with cyclic carbonate group enhance is presented. Based on arrangement basic amino residues surface FAP, bis(cyclic carbonate) containing flexible chain from CO 2 designed synthesized. This compound used modify porous hydrogels obtained via template‐etching process. then covalently within framework through reaction remaining groups, as evidenced by quartz crystal microbalance analysis. The modified carrier, PH3‐BC‐II, significantly improves activity achieving maximum conversion 70.0%, enzyme loading 125.3 mg g −1 (dry carrier). Furthermore, PH3‐BC‐II retains >50% its initial after eight consecutive cycles (total runtime 24 h) at high fatty substrate concentrations. study provides an effective strategy for constructing stable (photo)enzymes sustainable materials.

Language: Английский

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Immobilization and Application of Fatty Acid Photodecarboxylase in Deep Eutectic Solvents

ChemBioChem, Journal Year: 2022, Volume and Issue: 23(23)

Published: Oct. 12, 2022

Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been focus of extensive research, given ability to convert acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit applicability this biocatalyst, such as poor solubility substrates aqueous media, photostability, and impossibility reusing catalyst for several cycles. In work, we demonstrate use FAP non-conventional media a free enzyme an immobilized preparation. Namely, deep eutectic solvents (DESs) proof-of-concept immobilization commercial His-tag selective carrier, thorough study reaction conditions each case, well reusability studies shown. We observed almost complete selectivity towards C18 decarboxylation over C16 when used DES, with product analytical yield up 81 % whole cells. Furthermore, applying obtained yields >10-fold higher than ones media.

Language: Английский

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Eutectogels as Promising Materials in Biocatalysis

ChemCatChem, Journal Year: 2024, Volume and Issue: 16(12)

Published: May 3, 2024

Abstract The entrapment of deep eutectic solvents (DES) and systems into porous scaffolds renders a new class soft nonvolatile materials called eutectogels that have recently stepped the spotlight in different areas ranging from electronics to drug delivery. Recent progress use DES biocatalysis, where they been demonstrated improve substrate supply, conversion, enzyme stability, has opened an unparalleled opportunity exploit merits for immobilizing biological catalysts. resulting functional could outperform traditional hydrogels ionic liquid gels, offering fresh perspectives broaden application scope many enzymes. In this perspective, we go potential as innovative support biocatalytic reactions discuss applications these show plain benefits compared materials. Future directions newly developed technology are highlighted.

Language: Английский

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Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

Natural Products and Bioprospecting, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 23, 2024

Abstract Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl- γ -aminopropyl trimethoxy (R). R-SBA-15 employed to purify and immobilize recombinant β -glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step the first time. Optimum pH of constructed R-SBA-15@BgpA were 7.0, it has 20 ℃ higher optimal temperature than free enzyme. Relative activity still retained > 70% at 42 after 8-h incubation. The investigation on organic reagent resistance revealed that immobilized can maintain strong stability 15% DMSO. leaching test evaluation storage stability, only trace amount protein detected buffer 4 33 days, BgpA maintained 50% relative activity. It also demonstrated good reusability, with 76.1% remaining fourteen successive enzymatic hydrolyses epimedin A sagittatoside A. newly proposed strategy is an effective approach purification immobilization concurrently. addition, high efficiency application, suggesting its great feasibility potential produce bioactive compounds such as secondary glycosides or aglycones natural products. Graphical

Language: Английский

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Comprehensive insight into recent algal enzymes production and purification advances: Toward effective commercial applications: A review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137783 - 137783

Published: Nov. 1, 2024

Language: Английский

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