Enhancing Lipase Immobilization via Physical Adsorption: Advancements in Stability, Reusability, and Industrial Applications for Sustainable Biotechnological Processes

ACS Omega, Journal Year: 2024, Volume and Issue: 9(47), P. 46698 - 46732

Published: Nov. 14, 2024

Immobilization of lipases by physical adsorption improves their stability, recovery, and reusability in biotechnological processes. The present review provides an advanced bibliometric analysis a comprehensive overview research progress this field. By searching Web Science, 39,575 publications were analyzed, 325 relevant articles selected. Key journals, countries, institutions, authors identified. most cited focus on biofuel production industrial applications. revealed four themes with the biofuel. method is effective when appropriate support used. Despite decrease patent applications, interest remains high. Future studies should optimizing materials exploring new applications technique. detailed understanding immobilization adsorption.

Language: Английский

---

Latest Trends in Lipase-Catalyzed Synthesis of Ester Carbohydrate Surfactants: From Key Parameters to Opportunities and Future Development

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3727 - 3727

Published: March 27, 2024

Carbohydrate-based surfactants are amphiphilic compounds containing hydrophilic moieties linked to hydrophobic aglycones. More specifically, carbohydrate esters biosourced and biocompatible derived from inexpensive renewable raw materials (sugars fatty acids). Their unique properties allow them be used in various areas, such as the cosmetic, food, medicine industries. These multi-applications have created a worldwide market for biobased consequently expectations their production. Biobased can obtained processes, chemical synthesis or microorganism culture surfactant purification. In accordance with need more sustainable greener of these molecules by enzymatic pathways is an opportunity. This work presents state-of-the-art lipase action mode, focus on active sites proteins, then four essential parameters optimizing reaction: type lipase, reaction medium, temperature, ratio substrates. Finally, this review discusses latest trends recent developments, showing unlimited potential optimization syntheses.

Language: Английский

---

Investigation on solvent-free esterification of oleic acid by hemp tea waste-immobilized Candida Rugosa lipase

Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 392, P. 118 - 127

Published: July 3, 2024

Language: Английский

---

Waste Valorization in a Sustainable Bio‐Based Economy: The Road to Carbon Neutrality

Chemistry - A European Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract The development of sustainable chemistry underlying the quest to minimize and/or valorize waste in carbon‐neutral manufacture chemicals is followed over last four five decades. Both chemo‐ and biocatalysis have played an indispensable role this odyssey. particular developments protein engineering, metagenomics bioinformatics preceding three decades a crucial supporting facilitating widespread application both whole cell cell‐free biocatalysis. pressing need, driven by climate change mitigation, for drastic reduction greenhouse gas (GHG) emissions, has precipitated energy transition based on decarbonization defossilization organic production. latter involves biomass CO 2 as feedstock green electricity generated using solar, wind, hydroelectric or nuclear energy. use polysaccharides feedstocks will underpin renaissance carbohydrate with pentoses hexoses base bio‐based solvents polymers environmentally friendly downstream products. availability inexpensive solar led increasing attention electro(bio)catalysis photo(bio)catalysis which turn leading myriad innovations these fields.

Language: Английский

---

Energetical analysis of a method for the scale-up of a microwave flow P-esterification by recirculation

Reaction Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In a previous communication, we described flow chemical microwave implementation, where the reaction mixture is circulated.

Language: Английский

---

Biocatalysis: Important considerations for testing and evaluation of biocatalysts

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

---

An Accessible Continuous Flow Procedure for the Enantioselective Desymmetrization of a Key Precursor of Bioactive Myo‐Inositol Derivatives

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(12)

Published: March 1, 2025

Abstract We report herein continuous flow conditions for the TL‐IM lipase‐catalyzed enantioselective desymmetrization of 4,6‐di‐ O ‐benzyl‐ myo ‐inositol, a relevant precursor bioactive ‐inositol derivatives to D‐1‐ ‐acetyl derivative. This study represents first example an inositol under conditions. Given roles in cell biology research and medicine, practical, sustainable, efficient preparative procedures chiral inositols are highly desirable. Upon testing substrate solutions vinyl acetate/hexanes at various ratios passing them through packed‐bed lipase reactor, home‐made assembly, we identified optimal Reactions conducted 1.0:1.5 mixture 45 °C achieved conversions 97–95% with residence times 2.6–1.6 min, respectively. Notably, reactions more hydrophobic solvent system (1.0:2.5 acetate/hexanes) also showed high (95%) 1.6‐min time, highlighting benefits increased hydrophobicity. Productivity assessments reuse experiments confirmed robustness biocatalyst conditions, contrasting significant decline performance batch

Language: Английский

---

Active learning maps the emergent dynamics of enzymatic reaction networks.

Published: Aug. 29, 2024

The dynamic properties of enzymatic reaction networks (ERNs) are difficult to predict due the emergence allosteric interactions, product inhibitions and competition for resources, that all only materialize once have been assembled. Combining experimental kinetics studies with computational modelling allows us extract information on these emergent build predictive models. Here, we utilized pentose phosphate pathway demonstrate previously reported approaches construct maximally informative datasets can be significantly improved by pulsing both enzymes substrates into microfluidic flow reactors (instead only). Our method augments available from online databases, map behaviours a network.

Language: Английский

---

The scale-up of microwave flow syntheses by recirculation; The chlorine-free preparation of alkyl phenyl-H-phosphinates

Reaction Chemistry & Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

The MW flow esterification of phenyl- H -phosphinic acids with alcohols at ca. 150 °C in the presence 5% [bmim][PF 6 ] afforded target alkyl -phosphinates productivity 6–9 g h −1 by applying a recirculating mode operation.

Language: Английский

---

Biocatalysis in microfluidic systems: an experimental basis for data science

Reaction Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 9(8), P. 2028 - 2033

Published: Jan. 1, 2024

Accelerated development of new biocatalytic processes using data science from designed microfluidic experiments, aimed at scale-down exposure enzyme variants to conditions mimicking those found in industrial processes.

Language: Английский

---