Bayesian Optimization for an ATP-Regenerating In Vitro Enzyme Cascade
Catalysts,
Journal Year:
2023,
Volume and Issue:
13(3), P. 468 - 468
Published: Feb. 23, 2023
Enzyme
cascades
are
an
emerging
synthetic
tool
for
the
synthesis
of
various
molecules,
combining
advantages
biocatalysis
and
one-pot
multi-step
reactions.
However,
more
complex
enzyme
cascade
is,
difficult
it
is
to
achieve
adequate
productivities
product
concentrations.
Therefore,
whole
process
must
be
optimized
account
synergistic
effects.
One
way
deal
with
this
challenge
involves
data-driven
models
in
combination
experimental
validation.
Here,
Bayesian
optimization
was
applied
ATP-producing
-regenerating
consisting
polyphosphate
kinases.
The
co-substrate
concentrations
were
adjusted
ATP-dependent
reaction,
catalyzed
by
mevalonate
kinase
(MVK).
With
a
total
16
experiments,
we
able
iteratively
optimize
initial
components
used
improve
specific
activity
MVK
10.2
U
mg−1.
even
exceeded
results
reference
reaction
stoichiometrically
added
ATP
amounts,
which
8.8
mg−1
reached.
At
same
time,
also
improved
so
that
complete
yields
achieved.
Language: Английский
Modeling Enzymatic Cascade Reactions Immobilized in Plug‐Flow Reactors for Flow Biocatalysis
Chemie Ingenieur Technik,
Journal Year:
2024,
Volume and Issue:
96(6), P. 741 - 748
Published: April 16, 2024
Abstract
Flow
biocatalysis
has
emerged
as
a
promising
technology
for
the
sustainable
production
of
chemicals.
Multiple
immobilized
enzymes
are
often
used
biocatalysts
to
perform
enzymatic
cascade
reactions
in
flow
biocatalytic
systems.
When
designing
packed‐bed
reactors
with
multiple
enzymes,
distributed
porous
particles,
and
particles
catalyst
zones.
A
recently
developed
methodology
is
extended
selecting
spatial
immobilization
distributions
batch
reactors.
Mechanistic
models
compare
three
most
basic
designs
reactor,
i.e.,
mixed
individually
separated
co‐immobilized
enzymes.
For
first‐order
microkinetics,
design
was
found
always
outperform
other
two.
Separating
two
zones
proven
mixing
them
one
zone
any
positive
order
reaction
kinetic.
Language: Английский
Using Adsorption Energy Distribution for Parameter Estimation of Competitive Cofactor Coupled Enzyme Reaction
Processes,
Journal Year:
2023,
Volume and Issue:
11(9), P. 2686 - 2686
Published: Sept. 7, 2023
The
chemical
and
biotechnology
industries
are
facing
new
challenges
in
the
use
of
renewable
resources.
complex
nature
these
materials
requires
advanced
techniques
to
understand
kinetics
reactions
this
context.
This
study
presents
an
interdisciplinary
approach
analyze
cofactor
coupled
enzymatic
two-substrate
competitive
a
fast
efficient
manner.
By
studying
adsorption
energy
distribution
(AED),
it
is
possible
determine
individual
parameters
reaction
kinetics.
In
case
single
alcohol
reaction,
AED
able
identify
agreement
with
literature
few
experimental
data
points
compared
classical
methods.
analysis
can
automatically
number
competing
substrates,
whereas
traditional
nonlinear
regression
prior
knowledge
information
for
parameter
identification.
Language: Английский
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems
ChemElectroChem,
Journal Year:
2023,
Volume and Issue:
10(22)
Published: Oct. 5, 2023
Abstract
Adenosine‐5’‐triphosphate
(ATP)
plays
a
crucial
role
in
many
biocatalytic
reactions
and
its
regeneration
can
influence
the
performance
of
related
enzymatic
reaction
significantly.
Here,
we
established
electrochemically
coupled
ATP
by
pyruvate
oxidase
acetate
kinase
(ACK)
for
phosphorylation
mevalonate
catalyzed
kinase.
A
yield
84
%
product
phosphate
was
reached
total
turnover
number
ADP
68.
These
metrics
are
promising
development
an
economic
feasible
bioprocess
surpass
other
systems.
comparison
made
to
polyphosphate
kinases
(PPKs),
ACK,
kinase,
creatine
terms
donor
properties
exemplary
reactions.
Furthermore,
our
system
expanded
PPK
that
enables
AMP,
which
broaden
spectrum
applications
even
further.
Language: Английский
Boosting of Enzymatic Cascades by Intermediates: Theoretical Analysis and Model-Based Optimization
Published: Jan. 1, 2024
In
an
earlier
work
we
demonstrated
that
adding
intermediates
of
enzymatic
cascade
reaction
at
the
start
process
can
increase
space-time
yield
significantly.
This
method
was
coined
boosting
by
(BBI)
and
potentially
productivity
systems.
BBI
remains
largely
unexplored,
its
mechanism,
potential,
limitations
are
not
fully
understood.
present
work,
provide
a
theoretical
background
studying
four
chemical
networks
(CRN)
exhibit
intermediates.
We
study
dynamics
these
via
quasi
steady-state
approximation
numerical
solutions.
novel
graphical
be
used
to
deduce
which
boost
linear,
redox
neutral
networks.
Further,
demonstrate
how
such
designed
using
model-based
optimization.
Language: Английский
Boosting of enzymatic cascades by intermediates: Theoretical analysis and model-based optimization
Biochemical Engineering Journal,
Journal Year:
2024,
Volume and Issue:
210, P. 109440 - 109440
Published: July 27, 2024
A
recent
paper
demonstrated
that
adding
intermediates
of
an
enzymatic
cascade
reaction
at
the
start
process
can
increase
space-time
yield
significantly.
This
method
was
coined
boosting
by
(BBI)
and
potentially
productivity
systems.
BBI
remains
largely
unexplored,
its
mechanism,
potential,
limitations
are
not
fully
understood.
In
this
short
communication,
theoretical
background
is
revealed
studying
four
chemical
networks
exhibit
through
intermediates.
The
dynamics
these
studied
via
quasi-steady
state
approximation
numerical
solutions.
novel
graphical
be
used
to
deduce
which
boost
linear,
redox-neutral
presented.
Further,
how
such
a
designed
using
model-based
optimization
demonstrated.
Language: Английский