Published: March 30, 2023
mais bem caraterizado tem a seguinte organização Sep1-Sep2-Pnut-Pnut-
Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
1
Molecular & Cellular Proteomics, Journal Year: 2024, Volume and Issue: 23(3), P. 100730 - 100730
Published: Feb. 2, 2024
Vibrio species, the Gram-negative bacterial pathogens causing cholera and sepsis, produce multiple secreted virulence factors for infection pathogenesis. Among these is multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin that releases several critical effector domains with distinct functions inside eukaryotic host cells. One such domain, Rho inactivation domain (RID), has been discovered to catalyze long-chain N
Language: Английский
Citations
6
Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 901 - 901
Published: April 8, 2025
Background/Objectives: Schizophrenia and nicotine misuse are a comorbid condition that frequently develops during adolescence. Considering the role of nucleus accumbens (NAcc) as common neurobiological substrate for these psychiatric disorders, label-free proteomics was employed to identify NAcc deregulated proteins in male female mouse models schizophrenia with history adolescent exposure. Methods: Phencyclidine used model schizophrenia, minipump infusions were misuse. Results: Enrichment Reactome pathway protein–protein interaction analyses showed cytoskeleton associated synaptic plasticity mechanisms, energy metabolism, nervous system development affected both sexes. In particular, Ncam1 (Neural cell adhesion molecule 1) could be interest candidate marker disbalance. Its deregulation sexes suggests it lies at core comorbidity pathophysiology. When considering sex-selective effects, Cs (Citrate synthase) Mapk3 (Mitogen-activated protein kinase 3) identified exclusively mice, respectively. Since previously shown medial prefrontal cortex co-modeled mesocortical mesolimbic effect can inferred, supporting aberrant metabolism model. Conclusions: The current data provide insights into proteome disbalance an preclinical combined misuse, pointing relevant pathways early markers comorbidity.
Language: Английский
Citations
0
Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 91, P. 102442 - 102442
Published: Nov. 6, 2024
Language: Английский
Citations
3
Biological Chemistry, Journal Year: 2022, Volume and Issue: 404(1), P. 1 - 13
Published: Nov. 24, 2022
The septins are a conserved family of guanine nucleotide binding proteins, often named the fourth component cytoskeleton. They self-assemble into non-polar filaments and further higher ordered structures. Properly assembled septin structures required for wide range indispensable intracellular processes such as cytokinesis, vesicular transport, polarity establishment cellular adhesion. Septins belong structurally to P-Loop NTPases. However, unlike small GTPases like Ras, do not mediate signals effectors through GTP hydrolysis. role subsequent hydrolysis by is rather controversially debated. We compile here structural features from existing crystal- cryo-EM regarding protofilament formation, inter-subunit interface architecture These findings supplemented with summary available biochemical studies providing information fungal mammalian septins.
Language: Английский
Citations
12
Cytoskeleton, Journal Year: 2023, Volume and Issue: 80(7-8), P. 169 - 181
Published: Feb. 17, 2023
Abstract Septins are cytoskeletal GTPases that form nonpolar filaments and higher‐ordered structures they take part in a wide range of cellular processes. conserved from yeast to mammals but absent higher plants. The number septin genes vary between organisms usually complex heteropolymeric networks. Most septins known be capable GTP hydrolysis which may regulate dynamics. Knowledge on regulation function by post‐translational modifications is still its infancy. In this review article, we highlight the reported for 13 human discuss their implications functions. addition functionally investigated modifications, also try make sense modification code revealed large‐scale phospho‐proteomic datasets. Future studies determine how these isoform‐specific homology group specific affect structure function.
Language: Английский
Citations
7
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: July 12, 2024
Septins can function as scaffolds for protein recruitment, membrane-bound diffusion barriers, or membrane curvature sensors. are important cytokinesis, but their exact roles still obscure. In fission yeast, four septins (Spn1 to Spn4) accumulate at the rim of division plane rings. The octameric exocyst complex, which tethers exocytic vesicles plasma membrane, exhibits a similar localization and is essential deposition during cytokinesis. Without septins, spreads across absent from septum formation. These results suggest that physically interact proper localization. Indeed, we predicted six pairs direct interactions between septin subunits by AlphaFold2 ColabFold, most them confirmed co-immunoprecipitation yeast two-hybrid assays. Exocyst mislocalization in mistargeting secretory cargos, leads cell-separation delay mutants. Our indicate guide targeting complex on vesicle tethering cytokinesis through physical interactions.
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16269 - 16269
Published: Nov. 13, 2023
Adipose-derived stem cells (ADSCs) have incredible potential as an avenue to better understand and treat neurological disorders. While they been successfully differentiated into neural neurons, most such protocols involve 2D environments, which are not representative of in vivo physiology. In this study, human ADSCs were cultured 1.1 kPa polyethylene-glycol 3D hydrogels for 10 days with B27, CultureOne (C1), N2 supplements examine the differentiation using both chemical mechanical cues. Following treatment, cell viability, proliferation, morphology, proteome changes assessed. Results showed that viability was maintained during treatments, while continued proliferate over time, proliferation slowed down. Morphological between untreated treated observed. However, observed among exhibited cellular elongation co-alignment. Proteome analysis consistent early B27 C1 but N2. No significant detected immunocytochemistry, potentially indicating a greater period required. conclusion, treatment 3D-cultured PEG-based further enhances marker expression, however, supplementation
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14634 - 14634
Published: Sept. 27, 2023
Tobacco misuse as a comorbidity of schizophrenia is frequently established during adolescence. However, markers are still missing. Here, the method label-free proteomics was used to identify deregulated proteins in medial prefrontal cortex (prelimbic and infralimbic) male female mice modelled with history nicotine exposure Phencyclidine (PCP), model (SCHZ), combined an minipump infusions (NIC). The insults led worse outcomes than each insult separately when considering absolute number that exclusively ones. Partially shared Reactome pathways between sexes PCP, NIC PCPNIC groups indicate functional overlaps. Distinctively, differentially expressed reveal unique effects associated model. Interactome maps these identified sex-selective subnetworks, within which some stood out: for females, peptidyl-prolyl cis-trans isomerase (Fkbp1a) heat shock 70 kDa protein 1B (Hspa1b), both components oxidative stress subnetwork, gamma-enolase (Eno2), component energy metabolism subnetwork; males, amphiphysin (Amph), synaptic transmission subnetwork. These proposed be further investigated validated
Language: Английский
Citations
2
Published: Sept. 4, 2024
Septins can function as scaffolds for protein recruitment, membrane-bound diffusion barriers, or membrane curvature sensors. are important cytokinesis, but their exact roles still obscure. In fission yeast, four septins (Spn1 to Spn4) accumulate at the rim of division plane rings. The octameric exocyst complex, which tethers exocytic vesicles plasma membrane, exhibits a similar localization and is essential deposition during cytokinesis. Without septins, spreads across absent from septum formation. These results suggest that physically interact proper localization. Indeed, we predicted six pairs direct interactions between septin subunits by AlphaFold2 ColabFold, most them confirmed co-immunoprecipitation yeast two-hybrid assays. Exocyst mislocalization in mistargeting secretory cargos, leads cell-separation delay mutants. Our indicate guide targeting complex on vesicle tethering cytokinesis through physical interactions.
Language: Английский
Citations
0