Carbon-Based Bifunctional Heterogeneous Enzyme: Toward Sustainable Pollution Control DOI Creative Commons
Yuting Sun, Ming Guo, Shengnan Hu

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

We present a study on an immobilized functional enzyme (IFE), novel biomaterial with exceptional sustainability in utility, widely employed across various fields worldwide. However, conventional carriers are prone to eroding the active domain of IFE, thereby weakening its intrinsic activity. Consequently, there is burgeoning interest developing next-generation IFEs. In this study, we engineered carbon-based bifunctional heterogeneous (MIP-AMWCNTs@lipase) for intelligent recognition di(2-ethylhexyl)phthalate (DEHP), common plasticizer. The contains structural that both enriches and degrades DEHP. investigated dual-response performance enrichment specific removal imprinting factor carrier DEHP was 3.4, demonstrating selectivity rate reached up 94.2% over short period. exhibited robust activity, catalytic efficiency, excellent stability under harsh environmental conditions, retaining 77.7% initial lipase activity after 7 cycles. Furthermore, proposed stepwise reaction kinetic model based Michaelis-Menten equation enhance our understanding kinetics. Our employs dual-effect strategy molecular blotting immobilization establish method organic pollutants. These findings hold significant implications biomaterials science.

Language: Английский

Enzyme immobilization strategies for the design of robust and efficient biocatalysts DOI Creative Commons
José M. Guisán, Gloria Fernández‐Lorente, Javier Rocha‐Martín

et al.

Current Opinion in Green and Sustainable Chemistry, Journal Year: 2022, Volume and Issue: 35, P. 100593 - 100593

Published: Jan. 31, 2022

Different strategies for the preparation of efficient and robust immobilized biocatalysts are here reviewed. physico-chemical approaches discussed. i.- The stabilization enzyme by any kind immobilization on pre-existing porous supports. ii.- enzymes multipoint covalent attachment support surfaces. iii.- Additional immobilized-stabilized physical or chemical modification with polymers. These three can be easily developed when in In addition to that, these derivatives optimal develop reaction engineering reactor engineering. Stabilizations ranging between 1000 100,000 folds regarding diluted soluble reported.

Language: Английский

Citations

86
Biomass-derived functional materials as carriers for enzymes: towards sustainable and robust biocatalysts DOI Creative Commons
Meena Bisht,

Sarath Kumar Thayallath,

Pranav Bharadwaj

et al.

Green Chemistry, Journal Year: 2023, Volume and Issue: 25(12), P. 4591 - 4624

Published: Jan. 1, 2023

This review details the potential of BDFMs as promising carriers for enzymes design sustainable and robust biocatalysts with improved activity, stability, reusability.

Language: Английский

Citations

25
Extraction and Surfactant Properties of Glyoxylic Acid‐Functionalized Lignin DOI Creative Commons
Stefania Bertella, Monique Bernardes Figueirêdo, Gaia De Angelis

et al.

ChemSusChem, Journal Year: 2022, Volume and Issue: 15(15)

Published: May 9, 2022

The amphiphilic chemical structure of native lignin, composed by a hydrophobic aromatic core and hydrophilic hydroxy groups, makes it promising alternative for the development bio-based surface-active compounds. However, severe conditions traditionally needed during biomass fractionation make lignin prone to condensation cause lose groups in favour formation C-C bonds, ultimately decreasing lignin's abilities lower surface tension water/oil mixtures. Therefore, is often necessary further functionalize additional synthetic steps order obtain surfactant with suitable properties. In this work, multifunctional aldehyde-assisted glyoxylic acid (GA) was used prevent simultaneously introduce controlled amount carboxylic on backbone its use as surfactant. After fully characterizing extracted GA-lignin, activity measured several systems at different pH values. Then, stability water/mineral oil emulsions evaluated over course 30 days traditional photography microscopy imaging. Further, GA-lignin investigated formulation cosmetic hand cream industrially relevant ingredients. Contrary industrial lignins such Kraft did not alter color or smell formulation. Finally, compared other lignin-based fossil-based surfactants, showing that presented similar better properties some most commonly surfactants. overall results showed biopolymer can be made exclusively from renewable carbon, successfully one step lignocellulosic biomass. This an effective without modification, candidate new products.

Language: Английский

Citations

29
Extraction of lignin from corncob residue via a deep eutectic solvent for the preparation of nanoparticles by self-assembly DOI
Huan Zhang, Yi Shi,

Moying Li

et al.

Chemical Engineering Science, Journal Year: 2022, Volume and Issue: 256, P. 117694 - 117694

Published: April 29, 2022

Language: Английский

Citations

24
Perspectives on flow biocatalysis: the engine propelling enzymatic reactions DOI Creative Commons
Ana I. Benítez‐Mateos, Francesca Paradisi

Journal of Flow Chemistry, Journal Year: 2023, Volume and Issue: 14(1), P. 211 - 218

Published: Oct. 23, 2023

Abstract Flow biocatalysis has emerged as an empowering tool to boost the potential of enzymatic reactions towards more automatized, sustainable, and generally efficient synthetic processes. In last fifteen years, increasing number biocatalytic transformations carried out in continuous flow exemplified benefits that this technology can bring incorporate into industrial operations. This perspective aims capture a nutshell available methodologies for well discuss current limitations future directions field. Graphical abstract

Language: Английский

Citations

13
Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids DOI
Javier Santiago‐Arcos, Susana Velasco‐Lozano,

Eleftheria Diamanti

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(25), P. 9474 - 9489

Published: June 10, 2024

Cell-free biocatalysis is gaining momentum in producing value-added chemicals, particularly stepwise reaction cascades. However, the stability of enzyme cascades industrial settings often compromised when free enzymes are involved. In this study, we have developed a stable multifunctional heterogeneous biocatalyst coimmobilizing five on microparticles to transform 1,ω-diols into 1,ω-hydroxy acids. We improved operational efficiency and by fine-tuning loading spatial organization. Stability issues overcome through postimmobilization polymer coating. The general applicability demonstrated its scale-up both batch packed bed reactors, allowing product yield >80%. continuous process fed with H

Language: Английский

Citations

5
Dual Valorization of Lignin as a Versatile and Renewable Matrix for Enzyme Immobilization and (Flow) Bioprocess Engineering DOI Creative Commons
Ana I. Benítez‐Mateos, Stefania Bertella, Jean Behaghel de Bueren

et al.

ChemSusChem, Journal Year: 2021, Volume and Issue: 14(15), P. 3198 - 3207

Published: June 10, 2021

Lignin has emerged as an attractive alternative in the search for more eco-friendly and less costly materials enzyme immobilization. In this work, terephthalic aldehyde-stabilization of lignin is carried out during its extraction to develop a series functionalized lignins with range reactive groups (epoxy, amine, aldehyde, metal chelates). This expands immobilization pool enzymes (carboxylase, dehydrogenase, transaminase) by different binding chemistries, affording yields 64-100 %. As proof concept, ω-transaminase reversibly immobilized on polyethyleneimine-lignin integrated packed-bed reactor. The stability biocatalyst tested continuous-flow deamination reactions maintains same conversion 100 cycles. These results outperform previous tests covalently methacrylic resins, advantage that reversibility allows recycling reuse beyond inactivation. Additionally, in-line system also based added into downstream process separate reaction products catch-and-release. demonstrate fully closed-loop sustainable flow-biocatalytic exclusively lignin.

Language: Английский

Citations

31
Lipase immobilization using scalable and biocompatible lignin-based material as a carrier DOI
Huan Zhang, Rui Zhu, Yi Shi

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 193, P. 116241 - 116241

Published: Jan. 13, 2023

Language: Английский

Citations

12
Design and Applications of Enzyme-Linked Nanostructured Materials for Efficient Bio-catalysis DOI
Iqra Batool, Ayesha Iqbal, Muhammad Imran

et al.

Topics in Catalysis, Journal Year: 2023, Volume and Issue: 66(9-12), P. 649 - 675

Published: Jan. 3, 2023

Language: Английский

Citations

11
A review on the immobilization of bromelain DOI Creative Commons
Veymar G. Tacias-Pascacio, Daniel Castañeda‐Valbuena, Olga Luisa Tavano

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 273, P. 133089 - 133089

Published: June 14, 2024

This review shows the endeavors performed to prepare immobilized formulations of bromelain extract, usually from pineapple, and their use in diverse applications. extract has a potent proteolytic component that is based on thiol proteases, which differ depending location fruit. Stem fruit are areas where higher activity found. The edible origin this enzyme one features determines applications more significant degree. been wide diversity supports via different strategies (covalent bonds, ion exchange), also forming ex novo solids (nanoflowers, CLEAs, trapping alginate beads, etc.). preexisting nanoparticles as immobilization relevant, facilitates main enzyme, therapeutic (as wound dressing healing components, antibacterial or anticancer, mucus mobility control, A curiosity spores probiotic microorganisms adsorption, order have perfect vivo compatibility. Other outstanding stabilization wine versus haze during storage, mainly when chitosan. Curiously, scarcely applied production bioactive peptides.

Language: Английский

Citations

4