Metagenomic ecosystem monitoring of soft scale and mealybug infestations in Australian vineyards DOI Creative Commons
Christopher M. Ward, Cristóbal A. Onetto, Steven Van Den Heuvel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 30, 2023

Abstract Soft scale insects and mealybugs are phloem feeding Hemipterans that considered majors pests in agricultural horticultural settings throughout the world. Viticulturally, a major issue due to their ability secrete honeydew, which facilitates development of sooty mould for propensity as transmission vectors several viral diseases grapevine. To facilitate rapid identification quantification vineyard-associated metagenomic-based bioinformatic pipeline was developed generalised ecosystem monitoring automated assembly classification insect mitochondrial genomes from shotgun sequencing data using Barcode Life Database API. Parthenolecanium corni (European fruit scale), thought be absent Australian grapevines, identified dominant coccid species infesting all vines sampled, along with secondary infestation by Pseudococcus viburni (obscure mealybug) Pseudo. longispinus (long-tailed mealybug). In addition, parisitoidism Coccophagus scutellaris (Aphelinidae) wasps also detected. The discovery Parth. significant member infestations Australia has implications effective control strategies this important group pests.

Language: Английский

Monitoring the birds and the bees: Environmental DNA metabarcoding of flowers detects plant–animal interactions DOI Creative Commons
Joshua P. Newton, Philip W. Bateman, Matthew J. Heydenrych

et al.

Environmental DNA, Journal Year: 2023, Volume and Issue: 5(3), P. 488 - 502

Published: March 8, 2023

Abstract Animal pollinators are vital for the reproduction of ~90% flowering plants. However, many these pollinating species experiencing declines globally, making effective pollinator monitoring methods more important than ever before. Pollinators can leave DNA on flowers they visit, and metabarcoding environmental (eDNA) traces provides an opportunity to detect presence flower visitors. Our study, collecting from seven plant with diverse floral morphologies, eDNA analysis, illustrated value this novel survey tool. using three assays, including one developed in study target common bush birds, recorded animal visiting visual surveys conducted concurrently, bees, other species. We also a visit western pygmy possum; our knowledge is first simultaneously identify interaction insect, mammal, bird flowers. The highest diversity taxa was detected large inflorescence types found Banksia arborea Grevillea georgeana. demonstrates that ease sample collection robustness methodology has profound implications future management biodiversity, allowing us monitor both plants their attendant cohort potential pollinators. This opens avenues rapid efficient comparison biodiversity ecosystem health between different sites may provide insights into surrogate event declines.

Language: Английский

Citations

40
Environmental DNA metabarcoding from flowers reveals arthropod pollinators, plant pests, parasites, and potential predator–prey interactions while revealing more arthropod diversity than camera traps DOI Creative Commons
Mark D. Johnson, Aron D. Katz, Mark A. Davis

et al.

Environmental DNA, Journal Year: 2023, Volume and Issue: 5(3), P. 551 - 569

Published: March 29, 2023

Abstract Arthropods can strongly impact ecosystems through pollination, herbivory, predation, and parasitism. As such, characterizing arthropod biodiversity is vital to understanding ecosystem health, functions, services. Emerging environmental DNA (eDNA) methods targeting trace eDNA left behind on flowers have the potential track interactions. The goal of this study was determine extent which metabarcoding identify plant‐arthropod arthropod‐arthropod interactions assess compared conventional sampling. We deployed camera traps document activity specific flowers, sampled from those same then performed a analysis that targets partial fragment cytochrome c oxidase subunit I gene (COI) all present. found our detected small pollinators, plant pests, parasites, shed light predator–prey while detecting 55 species just 21 trapping. trapping survey, however, larger, more conspicuous nectarivores successfully. also explored ecology residual eDNA, finding rainfall had significant negative effect ability detect eDNA. Preliminary evidence indicates flower may amount be detected. provide clues highlights insights gained future studies. show valuable tool for not only pollinator communities but revealing among plants, predators. Future research should focus how improve detection large pollinators/nectivores studying further explore method's utility.

Language: Английский

Citations

27
Environmental DNA as an emerging tool in botanical research DOI Creative Commons
Mark D. Johnson, Joanna R. Freeland, Laura Parducci

et al.

American Journal of Botany, Journal Year: 2023, Volume and Issue: 110(2)

Published: Jan. 12, 2023

Over the past quarter century, environmental DNA (eDNA) has been ascendant as a tool to detect, measure, and monitor biodiversity (species communities), means of elucidating biological interaction networks, window into understanding patterns biodiversity. However, only recently potential eDNA realized in botanical world. Here we synthesize state applications systems with emphases on aquatic, ancient, contemporary sediment, airborne systems, focusing both single-species approaches multispecies community metabarcoding. Further, describe how abiotic biotic factors, taxonomic resolution, primer choice, spatiotemporal scales, relative abundance influence utilization interpretation results. Lastly, explore several areas opportunities for further development tools plants, advancing our knowledge efficacy, utility, cost-effectiveness, ultimately facilitating increased adoption analyses systems.

Language: Английский

Citations

25
Optimized DNA isolation from marine sponges for natural sampler DNA metabarcoding DOI Creative Commons
Lynsey R. Harper, Erika F. Neave, Graham S. Sellers

et al.

Environmental DNA, Journal Year: 2023, Volume and Issue: 5(3), P. 438 - 461

Published: Jan. 20, 2023

Abstract Marine sponges have recently been recognized as natural samplers of environmental DNA (eDNA) due to their effective water filtration and ubiquitous, sessile, regenerative nature. However, laboratory workflows for metabarcoding sponge tissue not optimized ensure that these achieve full potential community survey. We used a phased approach investigate the influence isolation procedures on biodiversity information recovered from sponges. In Phase 1, we compared three treatments residual ethanol preservative in alongside five extraction protocols. The results 1 informed which treatment protocol should be 2, where assessed effect starting mass success whether homogenization is required. indicated may contain unique and/or additional present tissue, but blotting dry generally more taxa generated sequence reads wild species. Tissue protocols performed best terms concentration, taxon richness, proportional read counts, non‐commercial was selected 2 cost‐efficiency greater recovery target taxa. overall, found required material does necessarily improve detection. These combined provide an procedure enhance marine assessment using sampler metabarcoding.

Language: Английский

Citations

12
Using individual‐based trait frequency distributions to forecast plant‐pollinator network responses to environmental change DOI Creative Commons
Aoife Cantwell‐Jones, Jason M. Tylianakis, Keith Larson

et al.

Ecology Letters, Journal Year: 2024, Volume and Issue: 27(1)

Published: Jan. 1, 2024

Abstract Determining how and why organisms interact is fundamental to understanding ecosystem responses future environmental change. To assess the impact on plant‐pollinator interactions, recent studies have examined effects of change individual interactions accumulate generate species‐level responses. Here, we review developments in using networks interacting individuals along with their functional traits, where are nested within species nodes. We highlight these individual‐level, trait‐based connect intraspecific trait variation (as frequency distributions multiple traits) dynamic communities. This approach can better explain interaction plasticity, changes probabilities network structure over spatiotemporal or other gradients. argue that only through appreciating such plasticity accurately forecast potential vulnerability follow this general guidance collect analyse high‐resolution data, hope improving predictions for targeted effective conservation.

Language: Английский

Citations

4
Environmental DNA profiling for detecting plant-insect interactions in endangered and native flora DOI Creative Commons
Andrew R. Pugh,

Max Trower,

Céline Mercier

et al.

Folia oecologica, Journal Year: 2025, Volume and Issue: 52(1), P. 82 - 90

Published: Jan. 1, 2025

Abstract Environmental DNA (eDNA) is an established technique for studying plant-insect interactions, that has so far had very limited use in exploring flower-visiting insect communities. This study provides important evidence of the effectiveness eDNA insects, proving its ability to provide a comprehensive overview pollinator communities beyond traditional observational methods. Our data revealed surprising diversity including both expected pollinators and possible non-pollinating species utilising pollen and/or nectar as nutritional resource. Native bees, such Leioproctus spp., various flies, those with uncertain roles pollination, were detected. also shed light on underexplored area nocturnal providing native moth involvement pollinating plant species. While there was no definitive rare insects visiting mānuka ( Leptospermum scoparium ) or Lophomyrtus this did reveal importance these resources flowers not just pollinators, but other key ecosystem.

Language: Английский

Citations

0
Molecular Identification of Wild Bees DOI
Mélodie Ollivier,

Giovanni Cilia,

Diego Cejas

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Unraveling the potential of environmental DNA for deciphering recent advances in plant–animal interactions: a systematic review DOI
Shahnawaz Hassan,

Sabreena,

Shahid Ahmad Ganiee

et al.

Planta, Journal Year: 2023, Volume and Issue: 258(6)

Published: Nov. 13, 2023

Language: Английский

Citations

6
Recovering plant-associated arthropod communities by eDNA metabarcoding historical herbarium specimens DOI Creative Commons
Manuel Stothut, Lisa Mahla,

Lennart Backes

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(18), P. 4318 - 4324.e6

Published: Aug. 27, 2024

Natural history collections are a priceless resource for understanding patterns and processes of biodiversity change in the Anthropocene.

Language: Английский

Citations

1
Utilizing herbarium specimens to assist with the listing of rare plants DOI Creative Commons
Brenda Molano‐Flores, Sara A. Johnson,

Paul B. Marcum

et al.

Frontiers in Conservation Science, Journal Year: 2023, Volume and Issue: 4

Published: Aug. 28, 2023

Funding for rare plant conservation is limited. In addition, many aspects of the biology and ecology plants are unknown. Therefore, low-cost data generation approaches to fill these gaps should be pursued. Herbarium specimens can used as a alternative learn about basic species. The information provided on herbarium labels has dramatically increased in recent decades include precise locality (i.e., latitude/longitude), exact dates, habitat, associated species, substrate. being digitized resulting images available via clearinghouses such GBIF SEINet. Already, have been develop habitat suitability models, predict range shifts, assess changes flower phenology due climate change. also provide wealth reproductive biotic interactions plants. this paper, we will demonstrate how accessed present practical application using populate an important federal listing document USA, Species Status Assessments (SSA). We examples from literature, well case studies our own research, collected where incorporate into SSAs. More generally, gleaned become part conservationist’s tool kit further knowledge past, present, future trends Additional species’ allows land managers conservationists make more informed decisions greater protection listed

Language: Английский

Citations

3