Environmental DNA methods for biosecurity and invasion biology in terrestrial ecosystems: Progress, pitfalls, and prospects
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
926, P. 171810 - 171810
Published: March 19, 2024
Language: Английский
Environmental DNA Methods for Detection of Varroa destructor in Honey Bee (Apis mellifera) Hives
Environmental DNA,
Journal Year:
2025,
Volume and Issue:
7(3)
Published: May 1, 2025
ABSTRACT
The
parasitic
mite,
Varroa
destructor
,
is
a
worldwide
problem
for
honey
bees
(
Apis
mellifera
).
recent
spread
of
this
pest
to
Australia,
which
was
one
the
few
varroa‐free
bee
populations
remaining,
highlights
importance
effective
biosecurity
methods
managing
incursions.
Detection
varroa
mite
environmental
DNA
(eDNA)
could
be
valuable
complementary
tool
increase
sensitivity
and
cost‐effectiveness
surveillance
as
it
spreads
new
areas.
Using
newly
developed
V.
quantitative
PCR
assay,
we
deployed
eDNA
testing
on
hives
in
New
Zealand
Australia
with
range
infestation
levels.
detection
using
hive
surface
swabs
comparable
conventional
alcohol
wash
method
used
by
beekeepers,
advantage
not
harming
bees.
However,
observed
greater
variability
detection,
particularly
entrance
swabs,
when
estimated
levels
were
below
1%.
introduced
from
Chatham
Islands
Wellington,
Zealand,
also
able
show
that
detectable
during
initial
invasion
hives.
Our
data
highlight
potential
incorporating
into
Australia's
programs
help
limit
mites
areas
detect
future
Language: Английский
Development of an Environmental DNA Assay for Prohibited Matter Weed Amazon Frogbit (Limnobium laevigatum)
Environments,
Journal Year:
2024,
Volume and Issue:
11(4), P. 66 - 66
Published: March 28, 2024
Environmental
DNA
(eDNA)
is
widely
used
for
detecting
target
species,
including
monitoring
endangered
species
and
the
presence
of
invasive
species.
Detecting
targeted
using
eDNA
approach
typically
carried
out
with
species-specific
qPCR
assays.
Amazon
frogbit
(Limnobium
laevigatum)
classified
as
a
State-Prohibited
Matter
Weed
in
NSW,
Australia.
It
fast-growing
perennial
aquatic
weed
that
outcompetes
native
plants,
leading
to
reduction
habitats
animals.
Early
detection
crucial
effective
management
this
In
study,
we
developed
assay
L.
laevigatum
based
on
rpoB
gene
sequence.
This
was
validated
against
25
non-target
terrestrial
found
be
species-specific,
positive
signal
exclusively
detected
laevigatum.
The
highly
sensitive
modelled
limits
3.66
copies
DNA/µL.
Furthermore,
our
environmental
samples
collected
from
field
sites
without
Our
an
tool
laevigatum,
which
will
enhance
efforts
monitor
control
weed.
Language: Английский