Biodiversity Assessment of a Mississippi River Backwater Complex Using eDNA Metabarcoding DOI Creative Commons

E. Ludwig,

Veronica M. Lee, Leah K. Berkman

et al.

Diversity, Journal Year: 2024, Volume and Issue: 16(8), P. 495 - 495

Published: Aug. 14, 2024

The backwater lowland habitats of large rivers, like the Mississippi River in North America, present complex and often inaccessible environments for traditional capture-based fish biodiversity sampling. Our knowledge assemblages fishes that occupy such is incomplete, this can compromise management efforts. We employed environmental DNA (eDNA) metabarcoding methods to sample a bottom wetland system assess ichthyofaunal diversity assemblage structure across habitat types, we compared our results with survey records same habitats. collected water samples spring fall 2022 from slough, ditch, shallow lake, bayou varied depth, vegetation, seasonal variability, connectivity channel. detected diverse array species included 51 taxa. Nearly all previously documented were using eDNA metabarcoding, increased number by more than third. Most ubiquitous range habitats, but there was also substantial structure, some exhibiting clear specificity. Fall sampling limited deeper where variation between minimal. metabarcode demonstrated be effective at detecting invasive as well uncommon species, which several conservation concern.

Language: Английский

Ichthyofaunal Metabarcoding in the Southern Appalachians: Use of eDNA Metabarcoding in Fish Surveys in Lotic Systems of the Great Smoky Mountains National Park with Comparisons to Historic Electrofishing Data DOI Creative Commons
Ben F. Brammell, Sara A. Brewer,

Karsner S. Fetter

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(4), P. 145 - 145

Published: March 22, 2025

eDNA appears well positioned to play a significant role in the future of biomonitoring, and need assess efficacy eDNA-based surveys variety habitats is increasing. We conducted an metabarcoding-based survey fish communities Great Smoky Mountains National Park (GSMNP), located eastern Tennessee western North Carolina. The GSMNP, widely recognized as biodiversity hotspot, encompasses 211,419 hectares Southern Appalachian with elevations up 2205 meters home approximately 73 species fish, including 12 families three classified endangered or threatened. collected 50 water samples first sixth order streams at 336 1462 meters, all major watersheds found park. was amplified utilizing two primer sets which each target differing regions 12S mitochondrial gene generate amplicons varying size (97 225 bp, respectively), sequencing expected read depth 400,000 reads per sample marker. detected total 40 species; these, 36 were set produces 97 bp amplicon, these only by this set. Species assemblages varied between stream orders, richness decreased increasing elevation increased order. Significant correlations observed biomass data from electrofishing monitoring (1984–2023) metabarcoding counts five seven examined, salmonids. demonstrated be effective assessing high-elevation lotic systems Appalachians, our results suggest that primers targeting shorter may exhibit greater ecosystems.

Language: Английский

Citations

0
Biodiversity Assessment of a Mississippi River Backwater Complex Using eDNA Metabarcoding DOI Open Access

E. Ludwig,

Veronica M. Lee, Leah K. Berkman

et al.

Published: July 24, 2024

The backwater lowland habitats of large rivers, like the Mississippi River in North America, present complex often inaccessible environments for traditional capture-based fish biodiversity sampling. Our knowledge assemblages fishes that occupy such are incomplete, and this can compromise management efforts. We employed environmental DNA (eDNA) metabarcoding methods to sample a bottom wetland system assess ichthyofaunal diversity, assemblage structure across habitat types, we compared our results survey records same habitats. collected water samples spring fall 2022 from slough, ditch, shallow lake bayou varied depth, vegetation, seasonal variability, connectivity channel. detected diverse array species included 51 taxa. Nearly all previously documented were by eDNA metabarcoding, increased number more than third. Most ubiquitous range habitats, but there was also substantial structure, with some exhibiting clear specificity. Fall sampling limited deeper where variation between minimal. metabarcode demonstrated be effective at detecting invasive as well uncommon species, which several conservation concern.

Language: Английский

Citations

2
Complete mitochondrial genomes of Notropis oxyrhynchus and Notropis buccula (Cypriniformes: Leuciscidae) DOI Creative Commons
Andrew T. Fields, Kevin W. Conway, M. Eileen Dolan

et al.

Mitochondrial DNA Part B, Journal Year: 2024, Volume and Issue: 9(11), P. 1569 - 1574

Published: Nov. 1, 2024

The Leuciscidae (minnows, shiners and relatives) is a diverse family of freshwater fishes with many species endangered due to anthropogenic stressors.

Language: Английский

Citations

1
Environmental DNA metabarcoding for whole community inventories of vertebrates in rivers of the midwestern United States DOI Creative Commons

Sophie Picq,

Amanda N. Curtis, Andrew D. George

et al.

Journal of Freshwater Ecology, Journal Year: 2024, Volume and Issue: 39(1)

Published: July 31, 2024

The application of environmental DNA (eDNA) methods to simultaneously study vertebrate diversity holds promise accelerate conservation efforts, especially in freshwater systems which are among the most imperiled world. Here, using eDNA sampling, we identify patterns biodiversity across different habitats Kankakee River watershed, one diverse lotic Illinois, USA. Our metabarcoding analyses identified 147 taxa, including 77 fishes, 38 birds, 24 mammals, five amphibians and three reptiles at 11 locations tributaries mainstem stretches upstream within State Park protected area. When compared seining, sampling consistently detected more fish species, non-native species. We also found that communities significantly varied taxonomic composition, showing an upstream-downstream shift along river as well tributary-specific assemblages. demonstrates ability single-marker document aquatic terrestrial large temperate ecosystems monitor areas.

Language: Английский

Citations

0
Biodiversity Assessment of a Mississippi River Backwater Complex Using eDNA Metabarcoding DOI Creative Commons

E. Ludwig,

Veronica M. Lee, Leah K. Berkman

et al.

Diversity, Journal Year: 2024, Volume and Issue: 16(8), P. 495 - 495

Published: Aug. 14, 2024

The backwater lowland habitats of large rivers, like the Mississippi River in North America, present complex and often inaccessible environments for traditional capture-based fish biodiversity sampling. Our knowledge assemblages fishes that occupy such is incomplete, this can compromise management efforts. We employed environmental DNA (eDNA) metabarcoding methods to sample a bottom wetland system assess ichthyofaunal diversity assemblage structure across habitat types, we compared our results with survey records same habitats. collected water samples spring fall 2022 from slough, ditch, shallow lake, bayou varied depth, vegetation, seasonal variability, connectivity channel. detected diverse array species included 51 taxa. Nearly all previously documented were using eDNA metabarcoding, increased number by more than third. Most ubiquitous range habitats, but there was also substantial structure, some exhibiting clear specificity. Fall sampling limited deeper where variation between minimal. metabarcode demonstrated be effective at detecting invasive as well uncommon species, which several conservation concern.

Language: Английский

Citations

0