3D Printed Multifunctional Biomimetic Bone Scaffold Combined with TP‐Mg Nanoparticles for the Infectious Bone Defects Repair

Small, Journal Year: 2024, Volume and Issue: unknown

Published: May 28, 2024

Abstract Infected bone defects are one of the most challenging problems in treatment due to high antibiotic failure rate and lack ideal grafts. In this paper, inspired by clinical cement filling treatment, α ‐c phosphate ( ‐TCP) with self‐curing properties is composited β ‐tricalcium constructed a bionic cancellous scaffolding system α/β‐tricalcium / low‐temperature 3D printing, gelatin preserved inside scaffolds as an organic phase, later loaded metal–polyphenol network structure tea polyphenol‐magnesium (TP‐Mg) nanoparticles. The mimic components mechanical strength (>100 MPa) based on ‐TCP through printing. Meanwhile, TP‐Mg exhibit significant inhibition Staphylococcus aureus S.aureus ) promote transition macrophages from M1 pro‐inflammatory M2 anti‐inflammatory phenotype. addition, composite scaffold also exhibits excellent bone‐enhancing effects synergistic effect Mg 2+ Ca . study, multifunctional ceramic ‐TCP@TP‐Mg) that integrates anti‐inflammatory, antibacterial, osteoinduction constructed, which promotes late regenerative healing while modulating early microenvironment infected defects, has promising application defects.

Language: Английский

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3D bioprinting: Advancing the future of food production layer by layer

Food Chemistry, Journal Year: 2025, Volume and Issue: 471, P. 142828 - 142828

Published: Jan. 9, 2025

Language: Английский

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Harnessing Native Blueprints for Designing Bioinks to Bioprint Functional Cardiac Tissue

iScience, Journal Year: 2025, Volume and Issue: 28(3), P. 111882 - 111882

Published: Jan. 23, 2025

Cardiac tissue lacks regenerative capacity, making heart transplantation the primary treatment for end-stage failure. Engineered cardiac tissues developed through three-dimensional bioprinting (3DBP) offer a promising alternative. However, reproducing native structure, cellular diversity, and functionality of requires advanced bioinks. Major obstacles in CTE (cardiac engineering) include accurately characterizing bioink properties, replicating microenvironment, achieving precise spatial organization. Optimizing properties to closely mimic extracellular matrix (ECM) is essential, as deviations may result pathological effects. This review encompasses rheological electromechanical bioinks function microenvironment design functional constructs. Furthermore, it focuses on improving characteristics, printability, bioinks, offering valuable perspectives developing new especially designed CTE.

Language: Английский

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Flexible Sono–Piezo Patch for Functional Sweat Gland Repair through Endogenous Microenvironmental Remodeling

ACS Nano, Journal Year: 2024, Volume and Issue: 18(31), P. 20283 - 20295

Published: July 22, 2024

Remodeling the endogenous regenerative microenvironment in wounds is crucial for achieving scarless, functional tissue regeneration, especially recovery of skin appendages such as sweat glands burn patients. However, current approaches mostly rely on use exogenous materials or chemicals to stimulate cell proliferation and migration, while remodeling a pro-regenerative remains challenging. Herein, we developed flexible sono-piezo patch (fSPP) that aims create an promote repair wounds. This patch, composed multifunctional fibers with embedded piezoelectric nanoparticles, utilized low-intensity pulsed ultrasound (LIPUS) activate electrical stimulation target tissue, resulting enhanced behaviors niche tissues cells, including peripheral nerves, fibroblasts, vasculatures. We further demonstrated effective wound healing regeneration injuries solely through physical stimulation. noninvasive drug-free therapeutic approach holds significant potential clinical treatment injuries.

Language: Английский

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Rheological Characterization and Printability of Sodium Alginate–Gelatin Hydrogel for 3D Cultures and Bioprinting

Biomimetics, Journal Year: 2025, Volume and Issue: 10(1), P. 28 - 28

Published: Jan. 4, 2025

The development of biocompatible hydrogels for 3D bioprinting is essential creating functional tissue models and advancing preclinical drug testing. This study investigates the formulation, printability, mechanical properties, biocompatibility a novel Alg-Gel hydrogel blend (alginate gelatin) use in extrusion-based bioprinting. A range compositions were evaluated their rheological behavior, including shear-thinning storage modulus, compressive which are crucial maintaining structural integrity during printing supporting cell viability. printability assessment 7% alginate-8% gelatin demonstrated that 27T tapered needle achieved highest normalized Printability Index (POInormalized = 1), offering narrowest strand width (0.56 ± 0.02 mm) accuracy (97.2%) at lowest pressure (30 psi). In contrast, 30R needle, with smallest inner diameter (0.152 (80 psi), resulted widest (0.70 0.01 (88.8%), resulting POInormalized 0.274. 30T 27R needles moderate performance, values 0.758 0.558, respectively. optimized alginate 8% favorable strength, compatibility MDA-MB-213 breast cancer cells, exhibiting high proliferation rates minimal cytotoxicity over 2-week culture period. formulation offers balanced approach, providing sufficient viscosity precision while minimizing shear stress to preserve health. work lays groundwork future advancements bioprinted models, contributing more effective tools screening personalized medicine.

Language: Английский

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In Situ Proefferocytosis Microspheres as Macrophage Polarity Converters Accelerate Osteoarthritis Treatment

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Efferocytosis in macrophages typically engages an anti‐inflammatory positive feedback regulatory mechanism. In osteoarthritis (OA), characterized by imbalanced inflammatory homeostasis, the proinflammatory state of immune microenvironment can be reversed through enhanced efferocytosis. This study develops situ proefferocytosis hydrogel microsphere (macrophage polarity converter, H‐C@IL) for OA treatment. Immunoliposomes (IL), CD16/32 antibody‐modified clodronate liposomes, are initially prepared using Re‐emulsion method. Then, IL is loaded into CCL19‐modified HAMA microspheres microfluidic technology. vitro, H‐C@IL specifically recruit M0 and M1 via CCL19, induce apoptosis secondary targeting with IL, provide “Find/Eat‐me” signals to enhance Additionally, it promotes macrophage polarization toward M2 phenotype. vivo, behavioral, imaging, histological analyses demonstrate that effectively facilitates M2, inhibits inflammation, cartilage regeneration. Mechanistically, enhances efferocytosis activating proteins such as PROS1 TIMD4 macrophages. Concurrently, signaling pathways, including PQLC2‐Arg‐Rac1 Pbx1/IL‐10, activated drive from M2. summary, situ, restores regeneration, offering a comprehensive treatment strategy OA.

Language: Английский

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Spinal Cord Injury Repair Based on Drug and Cell Delivery: from Remodeling Microenvironment to Relay Connection Formation

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101556 - 101556

Published: Feb. 4, 2025

Spinal cord injury (SCI) presents a formidable challenge in clinical settings, resulting sensory and motor function loss imposing significant personal societal burdens. However, owning to the adverse microenvironment limited regenerative capacity, achieving complete functional recovery after SCI remains elusive. Additionally, traditional interventions including surgery medication have series of limitations that restrict effectiveness treatment. Recently, tissue engineering (TE) has emerged as promising approach for promoting neural regeneration SCI, which can effectively delivery drugs into site cells improve survival differential. Here, we outline main pathophysiology events post injury, further discuss materials common assembly strategies used scaffolds treatment, expound on latest advancements treatment methods based drug cell detail, propose future directions repair with TE highlight potential applications.

Language: Английский

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Advances in Abiotic Tissue-Based Biomaterials: A Focus on Decellularization and Devitalization Techniques

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101735 - 101735

Published: April 1, 2025

This Review explores the growing and diversifying field of tissue-derived abiotic constructs for tissue engineering applications, with main focus on decellularization devitalization techniques principles. Acellular fractions derived from biological tissues, such as extracellular matrix (ECM), have long been considered a valuable approach generation numerous scaffolds more complex constructs. The removal cellular content has essential to prevent development adverse immunological reactions. Nevertheless, discovery promising features certain components sparked interest in use inactivated or devitalized several particularly regenerative medicine inflammation control. Devitalization described clinical but remains poorly explored terms vitro compared methods currently available. In this review, we present critically evaluate spectrum approaches whole-organs constructs, most prevalent techniques, discussion their implications composition, structure, potentially therapeutic properties. Processing methodologies achieve optimal cell-based materials effective characterization are discussed. application these healthcare, including examples commercially available products, is also addressed.

Language: Английский

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Electrohydrodynamic Direct‐Writing Micro/Nanofibrous Architectures: Principle, Materials, and Biomedical Applications

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Electrohydrodynamic (EHD) direct-writing has recently gained attention as a highly promising additive manufacturing strategy for fabricating intricate micro/nanoscale architectures. This technique is particularly well-suited mimicking the extracellular matrix (ECM) present in biological tissue, which serves vital function facilitating cell colonization, migration, and growth. The integration of EHD with other techniques been employed to enhance performance scaffolds, significant advancements have made development tailored scaffold architectures constituents meet specific requirements various biomedical applications. Here, comprehensive overview provided, including its underlying principles, demonstrated materials systems, A brief chronology along an examination observed phenomena that occur during printing process. impact biomaterial selection architectural topographic cues on also highlighted. Finally, major limitations associated are discussed.

Language: Английский

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Exploiting the Potential of Decellularized Extracellular Matrix (ECM) in Tissue Engineering: A Review Study

Macromolecular Bioscience, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 16, 2024

Abstract While significant progress has been made in creating polymeric structures for tissue engineering, the therapeutic application of these scaffolds remains challenging owing to intricate nature replicating conditions native organs and tissues. The use human‐derived biomaterials purposes closely imitates properties natural tissue, thereby assisting regeneration. Decellularized extracellular matrix (dECM) derived from tissues have become popular because their unique biomimetic properties. These dECM can enhance body's ability heal itself or be used generate new restoration, expanding beyond traditional transfers transplants. Enhanced knowledge how ECM scaffold materials affect microenvironment at injury site is expected improve clinical outcomes. In this review, recent advancements are explored relevant perspectives offered, highlighting development engineering various organs, such as skin, nerve, bone, heart, liver, lung, kidney.

Language: Английский

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