Strain‐Level Screening of Human Gut Microbes Identifies Three Lactobacillus Strains as Next‐Generation Antidiabetic Probiotics DOI Creative Commons
Yanan Yang, Mingxi Li, Fang Zhang

et al.

Food Frontiers, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

ABSTRACT Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by hyperglycemia. Probiotic supplementation improves glucolipid metabolism modulating the gut microbiota. However, current research lacks systematic understanding of overall hypoglycemic ability human microbes. First, high‐throughput screening platform based on HepG2 cells was developed to screen candidate strains with potential. Subsequently, we utilized T2DM mouse model investigate antidiabetic effects and mechanisms three selected Lactobacillus through biochemical analyses, histopathological RNA‐seq metagenomics analyses. We categorized ranked 4811 belonging 8 phyla, 17 orders, 68 genera, 241 species. The all tested showed significant normal distribution, several species efficacy were screened, primarily from Bifidobacterium . found that significantly improve glycolipid disorders in mice, include inhibiting hepatic gluconeogenesis via AKT–FOXO1–PEPCK/G6pase pathway activating GPR41 GPR43 bind short‐chain fatty acids, promoting glucagon‐like peptide‐1 (GLP‐1) secretion. These also regulate microbiota structure composition, contributing their effects. This study comprehensively examined for first time, resulting functional map hypoglycemia. Additionally, investigated strains. provides rich bacterial pool targeted development probiotics as potential treatments related diseases.

Language: Английский

Nanopore sequencing: flourishing in its teenage years DOI
Tianyuan Zhang, Hanzhou Li, Mian Jiang

et al.

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

20
Enhancement of gut barrier integrity by a Bacillus subtilis secreted metabolite through the GADD45A‐Wnt/β‐catenin pathway DOI Creative Commons
Shiqi Liu, Peiran Cai, Wenjing You

et al.

iMeta, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

Inflammatory bowel disease (IBD) represents a significant challenge to global health, characterized by intestinal inflammation, impaired barrier function, and dysbiosis, with limited therapeutic options. In this study, we isolated novel strain of Bacillus subtilis (B. subtilis) observed promising effects in protecting against disruption the gut barrier. Our findings indicate that enhancement function is primarily attributed its metabolites. We identified metabolite, 2-hydroxy-4-methylpentanoic acid (HMP), derived from B. subtilis, significantly improved function. also show growth arrest DNA damage 45A (GADD45A) key regulator mucosal integrity, which activated HMP subsequently activates downstream Wnt/β-catenin pathway. potentially contribute development probiotics-derived metabolites or targeted "postbiotics" as therapeutics for treatment prevention IBD other diseases associated dysfunction.

Language: Английский

Citations

5
From ancient wisdom to modern science: Gut microbiota sheds light on property theory of traditional Chinese medicine DOI
Yanan Yang, Jiaguo Zhan, Ying Cao

et al.

Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: 22(4), P. 413 - 444

Published: June 13, 2024

Language: Английский

Citations

7
The Gut Microbiota‐Xanthurenic Acid‐Aromatic Hydrocarbon Receptor Axis Mediates the Anticolitic Effects of Trilobatin DOI Creative Commons
Xiaoyu Wu,

Jiajia Wei,

Ran Wang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Current treatments for ulcerative colitis (UC) remain limited, highlighting the need novel therapeutic strategies. Trilobatin (TLB), a naturally derived food additive, exhibits potential anti‐inflammatory properties. In this study, dextran sulfate sodium (DSS)‐induced animal model is used to investigate effects of TLB on UC. It found significantly alleviates DSS‐induced UC in mice, as evidenced by reduction disease activity index, an increase colon length, improvement histopathological lesions. Furthermore, treatment results decrease proinflammatory cytokines and cytokines. mitigates modulating intestinal microbiota, particularly Akkermansia , which enhances tryptophan metabolism upregulates production xanthurenic acid (XANA). To confirm role TLB‐induced microbiota changes, experiments are performed with pseudogerm‐free mice fecal transplantation. also identified XANA key metabolite that mediates TLB's protective effects. Both markedly activate aromatic hydrocarbon receptor (AhR). Administration AhR antagonist abrogates their effects, thereby confirming involvement underlying mechanism. conclusion, study reveals mechanism through correcting imbalances, regulating metabolism, enhancing production, activating AhR.

Language: Английский

Citations

1
Isobutyrate confers resistance to inflammatory bowel disease through host-microbiota interactions in pigs DOI Creative Commons

Xiuyu Fang,

Haiyang Liu, Junling Liu

et al.

Research, Journal Year: 2025, Volume and Issue: 8

Published: Jan. 1, 2025

Supplementation with short-chain fatty acids (SCFAs) is a potential therapeutic approach for inflammatory bowel disease (IBD). However, the effects and mechanisms of action isobutyrate in IBD remain unclear. Clinical data indicate that fecal levels are markedly lower patients Crohn’s than healthy controls. Compared mice pigs, pigs colitis presented significantly levels. Furthermore, level was negatively correlated activity index. We speculate may play crucial role regulating host gut homeostasis. established model dextran sulfate sodium-induced which have gastrointestinal structure function similar to those humans; we performed multiomic analysis investigate on at both animal cellular validated results. Phenotypically, can alleviate diarrhea, bloody stools, weight loss, colon shortening caused by pigs. Mechanistically, increase relative abundance Lactobacillus reuteri , thereby increasing production indole-3-lactic acid, aryl hydrocarbon receptor expression downstream signaling pathways, Foxp3 + CD4 T cell recruitment colitis. Isobutyrate directly activate G protein-coupled 109A, promote Claudin-1, improve intestinal barrier function. In addition, SCFAs 3-hydroxybutyric acid inhibit TLR4/MyD88/NF-κB pathway suppress inflammation. conclusion, our findings demonstrate confers resistance through host–microbiota interactions, providing theoretical basis use alleviating

Language: Английский

Citations

1
Gut-lung Axis mediates asthma pathogenesis: Roles of dietary patterns and their impact on the gut microbiota DOI Creative Commons
Yanbo Liu, Ying Zhou, Haoyue Zhang

et al.

Experimental and Molecular Pathology, Journal Year: 2025, Volume and Issue: 142, P. 104964 - 104964

Published: April 8, 2025

The gut-lung axis, a vital signaling network linking the gastrointestinal and pulmonary systems, regulates immune responses progression of respiratory diseases. Nutritional components can modulate gut microbiome regulate synthesis critical intestinal microbial metabolites, which are essential for maintaining homeostasis supporting health. Conversely, poor dietary habits exacerbate asthma other conditions through modulation systemic inflammation responses. Dietary interventions, such as Mediterranean diet, reported to restore balance improve health by increasing production anti-inflammatory potentiating responses, preserving epithelial barrier integrity. In contrast, Western patterns, characterized high fat low fiber intake, disrupt diversity, resulting in increased levels pro-inflammatory metabolites that aggravate airway severity. This review aimed elucidate mechanisms underlying regulatory effects microbes their on asthma. Additionally, previous findings related axis have been summarized, providing insights into potential therapeutic strategies management.

Language: Английский

Citations

1
Compound oolong tea ameliorates lipid accumulation through AMPK-PPAR pathway of hepatic lipid metabolism and modulates gut microbiota in HFD induced mice DOI

Mingxiu Gong,

Yiwei Yuan, Xiao‐Lei Shi

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 196, P. 115041 - 115041

Published: Sept. 6, 2024

Language: Английский

Citations

6
Gut microbiota in women with polycystic ovary syndrome: an individual based analysis of publicly available data DOI Creative Commons
Yanan Yang,

Jiale Cheng,

Chongyuan Liu

et al.

EClinicalMedicine, Journal Year: 2024, Volume and Issue: 77, P. 102884 - 102884

Published: Oct. 18, 2024

Language: Английский

Citations

5
UPLC-MS metabolite profiling and antioxidant activity of Sanghuangporus sanghuang extract DOI Creative Commons
Weike Wang, Na Lu, Cheng Jiang

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18758 - e18758

Published: Jan. 22, 2025

Background The objective of the present study is to examine total phenolic and flavonoid content an ethanol extract Sanghuangporus sanghuang evaluate its phytochemical properties, antioxidant activity, capacity protect DNA from damage. This pharmaceutical/food resource mushroom may serve as a novel substitute functional food for health-conscious consumers, given promising source phenolics flavonoids. Methods S. (SEE) was evaluated contents, while UPLC-MS analysis used terpenoids, phenylpropanoid, flavonoids, steroidal, phenols identification, function prediction. Antioxidant anti-DNA damage activities were tested in vitro using ferric reducing power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS), protection assay. Results Conclusion Total (TPC) SEE 385.38 ± 1.36 mg GA/g extract, (TFC) 298.22 2.38 QE/g extract. extracts exhibited high free radical scavenging with relatively stronger activity. A 491 metabolites investigated by Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Most top 20 compounds predicted have various functions like antioxidant, anti-cancer anti-inflammatory. highlighted beneficial It contains potential natural that could be lead contender development medicines treatment wide range oxidative stress-related illnesses.

Language: Английский

Citations

0
Bioactivity profiling of Sanghuangporus lonicerinus : antioxidant, hypoglycaemic, and anticancer potential via in-vitro and in-silico approaches DOI Creative Commons
Yusufjon Gafforov, Sofija S. Bekić, Manzura Yarasheva

et al.

Journal of Enzyme Inhibition and Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 40(1)

Published: Feb. 24, 2025

This study investigates the mycochemical profile and biological activities of hydroethanolic (EtOH), chloroform (CHCl3), hot water (H2O) extracts Sanghuangporus lonicerinus from Uzbekistan. Antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), NO, FRAP assays, in vitro hypoglycaemic effects were evaluated through α-amylase α-glucosidase inhibition. Antiproliferative potential explored by analysing binding affinities EtOH H2O to estrogen receptor α (ERα), ERβ, androgen (AR), glucocorticoid (GR), with molecular docking providing structural insights. LC-MS/MS analysis revealed solvent-dependent phenolic profiles, extract containing highest total content (143.15 ± 6.70 mg GAE/g d.w.) best antioxidant capacity. The showed significant effects, 85.29 5.58% inhibition 41.21 0.79% α-amylase. Moderate ERβ suggests for estrogen-mediated cancer therapy, while strong AKR1C3 supports its therapeutic potential.

Language: Английский

Citations

0