Synergistic effects of diazotrophs and arbuscular mycorrhizal fungi on soil biological nitrogen fixation after three decades of fertilization

iMeta, Journal Year: 2023, Volume and Issue: 2(1)

Published: Jan. 27, 2023

Abstract Biological nitrogen (N) fixation (BNF) via diazotrophs is an important ecological process for the conversion of atmospheric N to biologically available N. Although soil play a dominant role in BNF and arbuscular mycorrhizal fungi (AMF) serve as helpers favor BNF, response diazotrophic communities different long‐term fertilizations AMF diazotrophs‐driven are poorly understood. Herein, 33‐year fertilization experiment wheat–maize intercropping system was conducted investigate changes rates, communities, their interactions after representative (chemical fertilizer, cow manure, wheat straw, green manure). We found remarkable increase rates more than three decades compared with nonfertilized soil, manure treatment rendered highest enhancement. The functionality strengthening mainly associated absolute abundance relative key cluster Module #0 (gained from co‐occurrence network species) such Skermanella Azospirillum . Furthermore, although positive correlations between were reduced under organic regimes, manuring could reverse decline within #0, this had relationship rate. This study suggests that promote select specific groups fixers certain areas. Our work provides solid evidence taxa interspecies will be largely favored fertilized strategy manure.

Language: Английский

---

Patterns and drivers of microbiome in different rock surface soil under the volcanic extreme environment

iMeta, Journal Year: 2023, Volume and Issue: 2(3)

Published: June 19, 2023

Soil microbial communities were investigated under the volcanic extreme environment. bacterial networks exhibited higher stability than fungal networks. Holocene granite had a more complex network basalt. pH and total protein key drivers of stability. Volcanic activity is great significance not only because it powerful strength shaping global landscape but also common cause geological ecological disturbances [1, 2]. The Wulanhada volcanoes in Inner Mongolia Northern China erupted during Quaternary period, which could be divided into late Pleistocene stages [3, 4]. rocks provide information regarding volcanic–tectonic processes certain tectonic environments from specific rock assemblages [3]. volcano consists dominantly alkali basalts with small amount [5]. eruptions lead to destruction surface vegetation loss primary soil; therefore, soil microorganisms tend search for habitats on [6, 7]. Previous studies have shown that colonization by microbes was influenced physicochemical properties rocks, such as mineral composition, permeability, pore structure, environmental factors, including water availability nutrient sources [8, 9]. Numerous research has progressed different localities rocks. Despite type can occur sedimentary limestone [10], halite [11], gypsum [12], igneous basalt [13, 14]. However, environment been widely reported, attracted our attention. some areas are dry atmosphere, extremely low temperature, utilization, high ultraviolet radiation [15, 16]. field belongs semi-arid continental monsoon climate middle temperate zone, characterized drought, cold wind, large temperature differences [17]. Thus, living may find suitable protect themselves [18]. influence extensively explored. For example, Atacama Desert, denitrification function maintained despite population being reduced [19]. adapt resist short-term perturbations Antarctic [20]. Studies unique regulation, fertility, ecosystem [21]. adaptable sensitive external changes, play an important role maintaining functions [22, 23]. Performing co-occurrence analyses enhance understanding [24, 25]. According previous studies, various pH, protein, nutrients, affect community especially [26]. main abiotic element microflora [27]. pattern distribution holds entire well individual microbiomes [28, 29]. In addition, exhibit significant conditions [30, 31]. As mentioned above, curious about variations soils environment, we studied [32]. This present study intended further (1) identify structure (2) explore major members inhabit rocky environments, (3) investigate factors communities. new opinions studying Volcano. results samples subjected Illumina paired-end sequencing showed reads 16 S rRNA gene sequences 447,762, average length 253.10 bp. While internal transcribed spacer sequence 472,558, 242.49 Then operational taxonomic units (OTUs) belonging mitochondrial, chloroplast, chlorophyte removed, then subsampled minimized sample, namely 283,590 (bacteria) 279,270 (fungi) (Figure 1A,B). richness assessed using sparse curves between plots based OTUs observed each plot. rarefaction all saturated, suggesting depth adequate represent almost (Supporting Information: Figure S1). curve principal component analysis result grouping reliable S2). composition periods same 1C,D). Bacterial groups primarily comprised phyla Actinobacteria (46.70%–52.90%), Proteobacteria (13.63%–16.10%), Acidobacteria (10.12%–12.23%) 1C). Ascomycota (58.30%–79.21%) Basidiomycota (26.21%–12.78%) 1D). Analysis similarity (ANOSIM) stable community. 2A). three identified linear discriminant effect size (LEfSe) method core statistical illustrated 2B,C. letters p, c, o, f, g represented phylum, class, order, family, genus, respectively. LEfSe revealed 63 clades (2 phyla, 4 classes, 10 orders, 17 families, 30 genera) 2B) 32 (1 3 12 2C). (LDA) there 33, 15, 15 bacteria basalt, granite, fungi, 11, 13, 8 phylotypes detected respectively S3). It shows fungi other, having biomarkers Regarding microorganisms, previously defined top 100 most abundant genera microbiome [33, 34]. used construct whole 2D–F) [35]. modules, where four modules; however, Late modules. topology Table S2) nodes same; links, connectivity (avgK), clustering coefficient (avgCC) highest path (GD) proportion positive correlation greater negative plots. contrast, modularity (M), GD, avgCC random lower empirical Given hubs, connectors, module hubs considered keystone taxa, peripherals, connectors value among-module (Pi) within-module (Zi) 2G–I); taxa. taxa accessorial unclassified_f_Micrococcaceae, Humicola, wb1_P19 2G); Luedemannella, Acidibacter, unclassified_o_Helotiales, Sporormiella, Keissleriella, Psychroglaciecola, MND1, Cladophialophora, Mortierella, unclassified_o_Gammaproteobacteria 2H); while Rubrobacter 2I). full forest model, diversity (Shannon index) 60.05% 33.95%, Six predictors community, plant chlorophyll content (plant TC) (9.8%), followed (8.7%), (soil PRO) (6.9%), organic phosphorus OP) (7.2%), peroxidase POD) (6.1%), carbon-to-nitrogen ratios C/N) (4.3%) 3A). Ten-fold cross-validation error minimal when variables included model S4a). exclusion PRO C/N greatest impact accuracy, least accuracy 3C). Additionally, five (9.6%), superoxide dismutase SOD) (9.5%), (8.9%), TP) (5.7%), (3.4%) 3B). S4b). 3D). closely associated PRO. Structural equation (SEM) direct indirect relationships multifunctionality 3E). Based forests, factors. diversity. Fungal multifunctionality. Furthermore, Sankey diagram contributor contributed 3F). Although at times rock, variation types relative abundance degree difference These caused chemical microenvironment they located. [16]. Interestingly, significantly differs other due inclusions [36]. Basalt magnesium better avgK 2D–F). species often related considerably [37]. natural ecosystems after succession, substantially influences biogeochemical cycling carbon, nitrogen, phosphorus, addition regulating [38, 39]. Different species, granite. soil, this few reported requires investigation. Therefore, speculate covering surface. number nodes, connections, avgK, consistent showing structurally diverse older younger [40]. archaea very (1%–2%), hence discussed subsequently. Actinobacteria, Proteobacteria, Acidobacteria, Ascomycota, dominant samples. Members these found survive [41]. Volcanoes need existing conditions. [14, 16], affects [42]. Actinobacteriota produce spores withstand promote weathering [43]. show 1), pro-colonizers commonly nutritionally endowed grow substrate [44]. explained rapid-growth prefer tardy-growth oligotrophic nutritional [16, 45, 46]. Similar research, attributed lack nutrients volcanic. Soils developed varying properties, strongly [47]. Microorganisms form close links plants [48, 49]. Plant TC factor affecting 3A), derivatives radical quenchers [50, 51]. study, exert irreversible effects [52]. driver [53]. regulates enzyme activities mineralization matter, biodegradation [54]. Notably, interactive stronger either single factor, multiple SEM priori visualizing causal variables, fitting data hypothesis [55]. multifunctionality, supports crucial alpha functions, resilient 39, 56]. Since experiments should carried out future field. results, soils; PH findings enhancing comprehension contribution Jin Chen Zishan Li conceived conducted analysis, wrote manuscript. Qingchen Xiao Xiaoyu analyzed data. Daolong Xu, Haijing Liu, Lumeng Chao, Hanting Qu, Yaxin Zheng, Xinyan Pengfei Wang designed experiments. Yuying Bao supervised process. All authors read approved final work supported National Natural Science Foundation (32201308, 31760005, 32160004), Autonomous Region (2021BS03030), Technology Plan (2020GG0079). declare no conflict interest. deposited NCBI Sequence Read Archive (SRA) accession PRJNA911355 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA911355) PRJNA911411 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA911411). Supplementary materials (methods, figures, tables, scripts, graphical abstract, slides, videos, Chinese translated version update materials) online DOI or iMeta http://www.imeta.science/. Please note: publisher responsible functionality any supporting supplied authors. Any queries (other missing content) directed corresponding author article.

Language: Английский

---

Biochar immobilized hydrolase degrades PET microplastics and alleviates the disturbance of soil microbial function via modulating nitrogen and phosphorus cycles

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 474, P. 134838 - 134838

Published: June 5, 2024

Language: Английский

---

Long-term green manuring increases soil carbon sequestration via decreasing qCO2 caused by lower microbial phosphorus limitation in a dry land field

Agriculture Ecosystems & Environment, Journal Year: 2024, Volume and Issue: 374, P. 109142 - 109142

Published: July 5, 2024

Language: Английский

---

Short-term continuous monocropping reduces peanut yield mainly via altering soil enzyme activity and fungal community

Environmental Research, Journal Year: 2023, Volume and Issue: 245, P. 117977 - 117977

Published: Dec. 21, 2023

Language: Английский

---

The dissolved organic matter from the co-decomposition of Chinese milk vetch and rice straw induces the strengthening of Cd remediation by Fe-modified biochar

Biochar, Journal Year: 2024, Volume and Issue: 6(1)

Published: March 25, 2024

Abstract Fe-modified biochar (FB) and co-using Chinese milk vetch rice straw (MR) are two effective ways for mitigating the cadmium (Cd) contamination in paddy fields southern China. Nevertheless, effects of FB combined with MR on Cd passivation mechanism remain unclear. In current study, strengthening induced by were found mechanisms extracted dissolved organic matter (DOM) from co-decomposition alleviation investigated through pot experiment adsorption experiment. Pot demonstrated that co-incorporating decreased available 23.1% increased iron plaque concentration 11.8%, resulting a 34.7% reduction concentrations brown compared addition FB. Furthermore, improved nutrients soil. The molecular characteristics DOM derived decomposition (DOM-MR) analyzed fluorescence excitation emission matrix spectroscopy-parallel factor analysis (EEM-PARAFAC) Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS). Results showed lignin/carboxylic-rich alicyclic molecules protein/amino sugar main compounds, potentially involved binding. Adsorption experiments revealed DOM-MR functional groups, specific surface area, negative charges FB, inducing both physisorption chemisorption Cd(II). maximum capacity after adding was 634 mg g −1 , 1.30 times without DOM-MR. This study suggested MR, could serve as an innovative practice simultaneous remediation soil fertilization Cd-polluted fields. It also provided valuable insights basis optimize performances enhance its potential immobilization. Graphical

Language: Английский

---

Optimizing cover crop practices as a sustainable solution for global agroecosystem services

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 5, 2024

The practice of cover crops has gained popularity as a strategy to improve agricultural sustainability, but its full potential is often limited by environmental trade-offs. Using meta-analytic and data-driven quantifications 2302 observations, we optimized crop practices evaluated their benefits for global agroecosystems. Cover have historically boosted yields, soil carbon storage, stability, also stimulated greenhouse gas emissions. However, combining them with long-term implementation (five years or more) climate-smart (such no-tillage) can enhance these services synergistically. A biculture legume non-legume crops, terminated 25 days before planting the next followed residue mulching, optimal portfolio. Such are projected increase agroecosystem multiservices 1.25%, equivalent annual gains 97.7 million metric tons in production, 21.7 billion dioxide sequestration, 2.41 erosion reduction. By 2100, continued could mitigate climate-related yield losses contribute climate neutrality stabilization, especially harsh underdeveloped areas. These findings underscore promising achieve synergy food security protection. sustainability. In this meta-analysis, authors find that may promote multiple while mitigating 2100.

Language: Английский

---

Green manuring relocates microbiomes in driving the soil functionality of nitrogen cycling to obtain preferable grain yields in thirty years

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 67(3), P. 596 - 610

Published: Dec. 5, 2023

Language: Английский

---

Soil Microorganisms in Agricultural Fields and Agronomic Regulation Pathways

Agronomy, Journal Year: 2024, Volume and Issue: 14(4), P. 669 - 669

Published: March 26, 2024

Agricultural soil microorganisms play a crucial role in farmland ecosystems and are integral to the material cycle these environments. The composition abundance of influenced by agronomic measures that alter microenvironment. These changes pivotal enhancing crop resistance, maximizing yield, facilitating nutrient cycling farmlands. Drawing on prior research advancements, this study systematically examined functions microorganisms, effects various their populations, ways which regulate article offers comprehensive agricultural influences microorganisms. Additionally, it outlines key areas for future farmlands, aiming provide valuable insights sustainable development ecosystems.

Language: Английский

---

Influence of Cover Crop Root Functional Traits on Sweet Potato Yield and Soil Microbial Communities

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 471 - 471

Published: Feb. 20, 2025

The symbiotic relationship between cover crops and soil microorganisms is closely linked to nutrient cycling crop growth within agroecosystems. However, how with different root functional traits influence microbial communities, properties, yields has remained understudied. This study assessed the of hairy vetch (HV) rapeseed (RP), along sweet potato yield, enzyme activity under red dryland conditions. High-throughput sequencing was also employed characterize diversity, composition, network structure bacterial fungal communities. According plant economic spectrum theory our research results on traits, HV can be identified as a resource-acquisitive crop, RP treatment resource-conservative crop. Although did not significantly increase rate reached 8.49%. Resource-conservative were associated increased pH, SOC, TP, which enhanced species diversity boosted populations Chloroflexi Alphaproteobacteria. In contrast, promoted proliferation Gammaproteobacteria. Network analysis indicated that facilitated complexity through intensified intra-community competition. Resource-acquisitive stability Collectively, these findings underscore distinct advantages varying in shaping Appropriate rotations effectively regulate communities hold potential enhance yield.

Language: Английский

---