Published: Jan. 1, 2024
Language: Английский
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 12, 2025
Flow cytometry commonly utilizes fluorescence labeling and extensive sample preparation to detect specific cell surface markers, making analysis under native conditions impractical. In this work, a label-free flow technique is presented that spatiotemporally resolves cell-surface interactions in antibody-coated microfluidic channels. Using computational imaging, numerous cells are tracked across large field of view (12 × 3 mm2) the resulting motion profiles used for phenotypic characterization. As proof-of-principle, experiments targeting T-cell receptor CD8 performed directly on cultures. Individual T-cells successfully 98% cases velocities 1-3 mm·s-1. 14 μm high channels coated with only nonspecific antibodies, both CD8-positive SUP-T1 CD8-negative Jurkat exhibit mostly constant velocities. contrast, using functionalized CD8-specific but not show temporary delays linked interaction. Cell classification based observed results clear contrast ratio 23.9 ± 11.6 (mean standard deviation) between at 1 The decreases higher as fewer interact due increased hydrodynamic lift. Our affirm our method's ability differentiate without prior or preparation.
Language: Английский
Citations
0
Advanced Therapeutics, Journal Year: 2024, Volume and Issue: 7(12)
Published: Nov. 9, 2024
Abstract The present review provides a comprehensive overview of the current state in vitro cancer studies, focusing on recent advancements and ongoing cell culture models analyses techniques. Cancer cells grow complex dynamic environment, interacting with various cellular components, such as stromal cells, cancer‐associated fibroblasts, immune extracellular matrix (ECM). ECM structural support unique characteristics essential for tumorigenesis. Accurately modeling this intricate tumor microenvironment precisely analyzing cell–cell cell–ECM interactions are crucial understanding progression therapeutic responses. Consequently, oncology research is advancing toward a) three‐dimensional models, b) single‐cell level analyses, c) live‐cell analyses. This aims to elucidate knowledge field, emphasizing benefits these innovative approaches offer over traditional two‐dimensional bulk endpoint measurements.
Language: Английский
Citations
2
ACS Measurement Science Au, Journal Year: 2024, Volume and Issue: 4(6), P. 678 - 688
Published: Sept. 17, 2024
Flow cytometry-based immunoassays are valuable in biomedical research and clinical applications due to their high throughput multianalyte capability, but adoption areas such as food safety environmental monitoring is limited by long assay times complex workflows. Rapid, simplified bead-based cytometric needed make these methods viable for point-of-need applications, especially with the increasing accessibility of miniaturized cytometers. This work introduces superparamagnetic hybrid polystyrene-silica core-shell microparticles promising alternatives conventional polymer beads competitive immunoassays. These beads, featuring specificity, sensitivity, excellent handling capabilities via magnetic separation, were evaluated three different antibodies binding methods, showing variations signal intensity based on antibody its attachment method. The optimal performance was achieved through a secondary approach, providing strong consistent signals minimal uncertainty. optimized protocol made it possible achieve detection limit 0.025 nM total time only 15 min successfully used detect ochratoxin A (OTA) raw flour samples. highlights potential versatile tools flow immunoassays, significant implications safety, animal health, monitoring, diagnostics.
Language: Английский
Citations
1
Optics and Lasers in Engineering, Journal Year: 2024, Volume and Issue: 186, P. 108731 - 108731
Published: Dec. 7, 2024
Language: Английский
Citations
1
Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2066 - 2066
Published: Dec. 13, 2024
Interstitial lung diseases (ILDs) are pathologies affecting the pulmonary interstitium and, less frequently, alveolar and vascular epithelia. Bronchoalveolar lavage (BAL) is commonly used in ILD evaluation since it allows sampling of lower respiratory tract. The prognostic value BAL cell counts unknown. Flow cytometry quantification lymphocytes neutrophils 1074 real-life consecutive patients were retrospectively correlated with clinical, radiological, anatomopathological, functional/spirometry, evolutionary data. Cut-offs predictive established at 7% 5% for neutrophils, respectively. Three risk stratification groups (Risk-LN) established: FAVORABLE (lymphocytes > < 5%), INTERMEDIATE (rest patients), UNFAVORABLE showing 75th percentile overall survival (OS) 10.0 ± 1.4, 5.8 0.6, 3.0 0.3 years (p 0.001), A scoring model combining Risk-LN age great capacity OS on fibrotic non-fibrotic ILDs proposed. This score an independent factor (HR = 1.859, p 0.002) complementary to fibrosis status 2.081, 0.001) type treatment. provides rapid accurate allowing establishment a that useful clinical management from time diagnosis.
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: July 9, 2024
Peyer's patches (PPs), which contain an abundance of B and T cells, play a key role in inducing pivotal immune responses the intestinal tract. PPs are defined as aggregated lymph follicles, consist multiple follicles (LFs) that may interact with each other synergistic manner. LFs thought to be spherical shape; however, characteristics their structure not fully understood. To elucidate changes individuals grow, we generated serial 2D sections from entire harvested mice at 2, 4, 10 weeks age performed 3D analysis using software, Amira. Although number was changed throughout experiment, volume surface area increased significantly, indicating develop continuously by recruiting even after weaning. In response dramatic environment weaning, development germinal centers (GCs) observed 4 (but 2 weeks) age. addition, GCs gradually began form away center close muscle layer where export lymphatic vessels develop. Importantly, LF joined adjacent LF; this feature preweaning nonactivated PPs. These results suggest have unique organization enhance functions, allowing cells free access egress smoothly periphery upon stimulation
Language: Английский
Citations
0
Extracellular Vesicles and Circulating Nucleic Acids, Journal Year: 2024, Volume and Issue: 5(3), P. 517 - 37
Published: Aug. 9, 2024
Infection with SARS-CoV-2, the virus responsible for COVID-19 diseases, can impact different tissues and induce significant cellular alterations. The production of extracellular vesicles (EVs), which are physiologically involved in cell communication, is also altered during COVID-19, along dysfunction cytoplasmic organelles. Since circulating EVs reflect state their cells origin, they represent valuable tools monitoring pathological conditions. Despite challenges detecting due to size specific compartment origin using methodologies, flow cytometry has proven be an effective method assessing role COVID-19. This review summarizes involvement plasmatic patients individuals Long COVID (LC) affected by post-acute sequelae SARS-CoV-2 infection (PASC), highlighting dual exerting both pro- antiviral effects. We emphasize how cytometry, its multiparametric approach, employed characterize EVs, particularly infectious diseases such as suggest potential chronic impairments post-infection.
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0