miRNA Involvement in Cerebral Ischemia-Reperfusion Injury

Frontiers in Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: June 10, 2022

Cerebral ischemia reperfusion injury is a debilitating medical condition, currently with only limited amount of therapies aimed at protecting the cerebral parenchyma. Micro RNAs (miRNAs) are small, non-coding RNA molecules that via RNA-induced silencing complex either degrade or prevent target messenger from being translated and thus, can modulate synthesis proteins. In neurological field, miRNAs have been evaluated as potential regulators in brain development processes pathological events. Following ischemic hypoxic stress, cellular molecular events initiated dysregulate different miRNAs, responsible for long-terming progression extension neuronal damage. Because their ability to regulate proteins, emerge possible therapeutic strategy limiting damage following event. This review aims summarize recent literature evidence involved signaling modulating ischemia-reperfusion injuries, thus pointing repair mechanisms. An in-depth overview pathways involvement specific could provide future perspectives neuroprotective agents targeting these miRNAs.

Language: Английский

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Design, synthesis and molecular docking of novel substituted azepines as inhibitors of PI3K/Akt/TSC2/mTOR signaling pathway in colorectal carcinoma

Bioorganic Chemistry, Journal Year: 2022, Volume and Issue: 131, P. 106299 - 106299

Published: Dec. 1, 2022

Language: Английский

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Exosomal miR‐128‐3p reversed fibrinogen‐mediated inhibition of oligodendrocyte progenitor cell differentiation and remyelination after cerebral ischemia

CNS Neuroscience & Therapeutics, Journal Year: 2023, Volume and Issue: 29(5), P. 1405 - 1422

Published: Feb. 8, 2023

Abstract Aims To investigate the role of exosomal miR‐128‐3p in promoting fibrinogen‐mediated inhibition oligodendrocyte progenitor cell (OPC) differentiation and therapeutic potential cerebral ischemia. Methods Mouse models middle artery occlusion (MCAO) were established as described previously. MCAO was treated with fibrinogen exosomes by stereotactically injecting into left stratum. cortical OPCs used for mRNA miRNA sequencing analysis. Exosomes isolated from neural stem cells (NSCs) mice. Results Fibrinogen deposition suppressed remyelination after inhibited OPC activating ACVR1, bone morphogenetic protein (BMP) signaling type I receptor. In vitro, miR‐sequencing verification studies revealed that is associated BMP mediated ACVR1. Additionally, transfer NSC‐derived to significantly increased myelin basic expression signaling. Furthermore, protected against fibrinogen‐induced demyelination related signaling, reduced infarct volume, improved neurological function MCAO. Conclusions inhibits ischemic damage promotes OLs suppressing indicating represents a target stroke.

Language: Английский

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Circ_0000647 promotes cell injury by modulating miR-126-5p/TRAF3 axis in oxygen-glucose deprivation and reperfusion-induced SK-N-SH cell model

International Immunopharmacology, Journal Year: 2022, Volume and Issue: 104, P. 108464 - 108464

Published: Jan. 10, 2022

Language: Английский

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Effects of dexmedetomidine on cognitive dysfunction and neuroinflammation via the HDAC2/HIF‐1α/PFKFB3 axis in a murine model of postoperative cognitive dysfunction

Journal of Biochemical and Molecular Toxicology, Journal Year: 2022, Volume and Issue: 36(6)

Published: May 2, 2022

Inhibition of histone deacetylase (HDAC) may be a useful approach in the treatment disorders characterized by cognitive dysfunction. Dexmedetomidine (DEX), an α2-adrenoceptor (α2-AR) agonist, has demonstrated neuroprotective effects. Here, we attempted to investigate protective effects DEX on postoperative dysfunction (POCD) involving HDAC2. Male C57BL/6 mice were selected develop POCD model, where HDAC2, HIF-1α, and PFKFB3 expression was quantified. administered before modeling. Then function evaluated with open field Y-maze tests. Meanwhile, lipopolysaccharide (LPS) employed induce BV-2 microglial cells simulate inflammatory response. The contents TNF-α, IL-6, IL-10 measured enzyme-linked immunosorbent assay (ELISA) mouse serum cell supernatant. Abundant confirmed (p < 0.05). Cognitive could alleviated following pharmacological inhibition HDAC2 FK228 Mechanistically, upregulated HIF-1α promoted secretion factors LPS-exposed attenuated neuroinflammation resulting decreasing HIF-1α-dependent upregulation In conclusion, DEX-regulated play inhibitory role through regulation HIF-1α/PFKFB3 axis.

Language: Английский

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miR-181a targets PTEN to mediate the neuronal injury caused by oxygen-glucose deprivation and reoxygenation

Metabolic Brain Disease, Journal Year: 2023, Volume and Issue: 38(6), P. 2077 - 2091

Published: May 13, 2023

Language: Английский

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Role of non-coding RNAs in neuroblastoma

Cancer Gene Therapy, Journal Year: 2023, Volume and Issue: 30(9), P. 1190 - 1208

Published: May 22, 2023

Language: Английский

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Plant based radioprotectors as an adjunct to radiotherapy: advantages and limitations

Journal of Radiological Protection, Journal Year: 2022, Volume and Issue: 42(2), P. 021001 - 021001

Published: Feb. 7, 2022

Radioprotectors are agents that have the potential to act against radiation damage cells. These equally invaluable in protection, both intentional and unintentional exposure. It is however, complex use a universal radioprotector could be beneficial diverse contexts such as radiotherapy, nuclear accidents, space travel, each of these circumstances unique requirements. In clinical setting used increase efficacy cancer treatment. The protective agent must selectively normal healthy cells while enhancing imparted on context plant-based compounds offer more reliable solution over synthetic ones former less expensive, toxic, possess synergistic phytochemical activity, environmentally friendly. Phytochemicals with radioprotective anticancer properties may enhance treatment by two-fold. Hence, plant based promising field progress forward, expand boundaries protection. This review an account phytochemicals complications encountered development ideal adjunct radiotherapy.

Language: Английский

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Chitosan oligosaccharide attenuates hepatic steatosis in HepG2 cells via the activation of AMP‐activated protein kinase

Journal of Food Biochemistry, Journal Year: 2022, Volume and Issue: 46(5)

Published: Feb. 20, 2022

Chitosan oligosaccharides (COSs), oligomers of decomposed chitosan possess many biological functions including immunomodulatory, antitumor, and antiinflammation. The aim this study was to investigate the protective effects COS against free fatty acid (FFA)-induced cellular hepatic steatosis underlying mechanisms in HepG2 cells. Results showed that significantly reduced lipid contents elevated activities antioxidant enzymes total-superoxide dismutase, glutathione peroxidase, catalase FFA-stimulated phosphorylated acetyl-CoA carboxylase both mRNA protein levels lipogenesis markers synthase sterol regulatory element-binding 1c. also increased expression oxidation-related factors carnitine palmitoyltransferase 1A, acyl-coenzyme A oxidase 1, peroxisome proliferators-activated receptor α. Besides, markedly AMP-activated kinase (AMPK). inhibition enhancement oxidation induced by were all blocked AMPK antagonist (compound C), showing attenuation dependent on activation. In conclusion, attenuated via suppressing synthesis enhancing oxidation. involved alleviation COS. These results indicated might be used as a potential ingredient ameliorate nonalcoholic liver disease. Practical applications Nonalcoholic disease (NAFLD) has been regarded pathological fat deposition liver, which includes range pathologies, from steatohepatitis, fibrosis, carcinoma. Our findings demonstrated (COS) improving metabolism. suppressed enhanced molecular mechanism whereby elicited these beneficial proved through modulation upstream kinase, kinase. This provides new knowledge support food supplement for prevention NAFLD.

Language: Английский

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Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture

Biomolecules, Journal Year: 2022, Volume and Issue: 12(4), P. 515 - 515

Published: March 29, 2022

Charcot-Marie-Tooth (CMT) syndrome is the most common progressive human motor and sensory peripheral neuropathy. CMT type 1E a demyelinating neuropathy affecting Schwann cells due to peripheral-myelin-protein-22 (PMP22) mutations, modelized by Trembler-J mice. Curcumin, natural polyphenol compound obtained from turmeric (Curcuma longa), exhibits dose- time-varying antitumor, antioxidant neuroprotective properties, however, neurotherapeutic actions of curcumin remain elusive. Here, we propose as possible treatment capable enhancing cellular detoxification mechanisms, resulting in an improvement neurodegenerative phenotype. Using refined method for obtaining enriched cell cultures, evaluated action low dose on PMP22 expression, chaperones autophagy/mammalian target rapamycin (mTOR) pathways wild-type genotypes. In cells, resulted strong stimulation chaperone macroautophagy pathway, whereas modulation ribophagy showed mild effect. However, despite promising effects neurological diseases, demonstrate that could be impaired irreversible impact ethanol used vehicle necessary administration. These results contribute expanding our still limited understanding biology neurobiology expose intrinsic lability genotype. Furthermore, they unravel interesting physiological mechanisms resilience relevant pharmacological Tremble J phenotype with ethanol. We conclude analysis itself essential inescapable step comprehensibly assess full potential therapeutic purposes.

Language: Английский

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