Physiological Research,
Journal Year:
2024,
Volume and Issue:
1/2024, P. 127 - 138
Published: March 6, 2024
MicroRNAs
have
been
shown
to
potentially
function
in
cerebral
ischemia/reperfusion
(IR)
injury.
This
study
aimed
examine
the
expression
of
microRNA-320
(miR-320)
IR
injury
and
its
involvement
mitochondrial
function,
oxidative
stress,
inflammatory
responses
by
targeting
HMGB1/
NF-κB
axis.
Sprague-Dawley
rats
were
subjected
middle
artery
occlusion
simulate
The
miR-320
was
assessed
using
qRT-PCR.
Neurological
infarct
volume,
cytokines
evaluated
relevant
methods,
including
staining,
fluorometry,
ELISA.
HMGB1
analyzed
through
Western
blotting.
levels
miR-320,
HMGB1,
neurological
deficits,
infarction
significantly
higher
after
induction.
Intracerebral
overexpression
resulted
substantial
increased
elevated
8-isoprostane,
NF-κBp65,
TNF-α,
IL-1β,
ICAM-1,
VCAM-1,
expression.
It
also
promoted
loss
membrane
potential
ROS
while
reducing
MnSOD
GSH
levels.
Downregulation
inhibition
activity
reversed
outcomes
MiR-320
plays
a
negative
role
regulating
inflammatory/oxidative
reactions
induced
enhancing
modulating
function.
Frontiers in Neurology,
Journal Year:
2024,
Volume and Issue:
14
Published: Jan. 18, 2024
Brain
diseases
have
become
one
of
the
leading
roots
mortality
and
disability
worldwide,
contributing
a
significant
part
disease
burden
on
healthcare
systems.
The
blood-brain
barrier
(BBB)
is
primary
physical
biological
obstacle
that
allows
only
small
molecules
to
pass
through
it.
Its
selective
permeability
challenge
in
delivering
therapeutics
into
brain
for
treating
dysfunction.
It
estimated
2%
new
central
nervous
system
(CNS)
therapeutic
compounds
can
cross
BBB
achieve
their
targets.
Scientists
are
exploring
various
approaches
develop
effective
cargo
delivery
vehicles
promote
better
targeting
with
minimal
off-target
side
effects.
Despite
different
synthetic
carriers,
natural
systems,
“exosomes,”
now
employed
transport
drugs
BBB.
Exosomes
naturally
occurring
extracellular
vesicles
(EVs)
unique
advantages
as
disorders.
They
beneficial
innate
aspects
biocompatibility,
higher
stability,
ability
BBB,
low
cytotoxicity,
immunogenicity,
homing
potential,
targeted
delivery,
reducing
off-site
target
In
this
review,
we
will
discuss
limitations
carriers
utilization
exosomes
brain-targeted
highlight
methods
modifying
exosome
surfaces
drug
loading
exosomes.
We
also
enlist
neurodegenerative
disorders
genetically
modified
treatment.
Mediators of Inflammation,
Journal Year:
2024,
Volume and Issue:
2024, P. 1 - 16
Published: March 21, 2024
Background.
Cerebral
ischemia–reperfusion
injury
is
a
common
complication
of
ischemic
stroke
that
affects
the
prognosis
patients
with
stroke.
The
lipid-soluble
diterpene
Tanshinone
IIA,
which
was
isolated
from
Salvia
miltiorrhiza,
has
been
indicated
to
reduce
cerebral
injury.
In
this
study,
we
investigated
molecular
mechanism
IIA
in
alleviating
reperfusion-induced
brain
Methods.
Middle
artery
occlusion
animal
models
were
established,
and
neurological
scores,
tetrazolium
chloride
staining,
volume
quantification,
wet
dry
water
content
measurement,
Nissl
enzyme-linked
immunosorbent
assay,
flow
cytometry,
western
blotting,
reverse
transcription–quantitative
polymerase
chain
reaction
performed.
viability
cells
measured
by
3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl
bromide
assays,
while
cell
damage
lactate
dehydrogenase
release
vitro
oxygen
glucose
deprivation
model.
addition,
used
evaluate
therapeutic
effect
on
ischemia/reperfusion
(I/R)
induced
injury,
as
well
its
effects
inflammatory
response
neuronal
apoptosis,
vivo
vitro.
Furthermore,
study
validated
targeting
relationship
between
miR-124-5p
FoxO1
using
dual
luciferase
assay.
Finally,
examined
role
perspective
inhibiting
or
increasing
levels.
Results.
After
treatment
middle
occlusion–reperfusion
(MCAO/R)
rats,
infarction
reduced,
decreased,
nerve
function
rats
significantly
improved,
reduced.
effectively
inhibited
I/R-induced
is,
it
expression
cytokines
IL-1β,
IL-6,
TNF-α,
decreased
apoptotic
protein
Bax
Cleaved-caspase-3,
promoted
antiapoptotic
Bcl-2.
oxygen-glucose
deprivation/reoxygenation
(OGD/R)
model,
also
factors
occurrence
apoptosis.
miR-124-5p.
Transfection
mimic
same
positive
OGD
cells,
transfection
inhibitor
opposite
effect.
negatively
regulates
expression.
Inhibition
overexpression
can
weaken
inhibitory
I/R,
inhibition
further
IIA.
Conclusion.
alleviates
ischemic–reperfusion
neuroinflammation
through
miR-124-5p/FoxO1
axis.
This
finding
provides
theoretical
basis
for
mechanistic
research
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(16), P. 12899 - 12899
Published: Aug. 17, 2023
In
recent
times,
there
has
been
a
significant
increase
in
researchers’
interest
the
functions
of
microRNAs
and
role
these
molecules
pathogenesis
many
multifactorial
diseases.
This
is
related
to
diagnostic
prognostic
potential
microRNA
expression
levels
as
well
prospects
using
it
personalized
targeted
therapy.
review
literature
analyzes
existing
scientific
data
on
involvement
molecular
cellular
mechanisms
underlying
development
pathologies
such
Alzheimer’s
disease,
cerebral
ischemia
reperfusion
injury,
dysfunction
blood–brain
barrier.
World Journal of Gastrointestinal Surgery,
Journal Year:
2025,
Volume and Issue:
17(2)
Published: Jan. 20, 2025
Intestinal
ischemia-reperfusion
injury
(IIRI)
is
a
complex
and
severe
pathophysiological
process
characterized
by
oxidative
stress,
inflammation,
apoptosis.
In
recent
years,
the
critical
roles
of
extracellular
matrix
(ECM)
genes
microRNAs
(miRNAs)
in
IIRI
have
garnered
widespread
attention.
This
review
aims
to
systematically
summarize
diagnostic
therapeutic
potential
ECM
gene
sets
miRNA
regulatory
networks
IIRI.
First,
we
molecular
mechanisms
IIRI,
focusing
on
dual
role
tissue
repair
processes.
The
expression
changes
functions
components
such
as
collagen,
elastin,
metalloproteinases
during
progression
are
deeply
analyzed.
Second,
miRNAs
particularly
miR-125b
miR-200a
regulating
apoptosis,
remodeling.
Additionally,
this
discusses
biomarkers
treatment
strategies
based
miRNAs.
We
extensively
evaluate
prospects
miRNA-targeted
therapy
component
modulation
preventing
treating
emphasizing
clinical
translational
these
emerging
therapies.
conclusion,
provides
new
directions
for
further
research,
necessitating
additional
basic
studies
validate
expand
findings
improving
outcomes
patients.
Biomedicines,
Journal Year:
2025,
Volume and Issue:
13(2), P. 449 - 449
Published: Feb. 12, 2025
Background/Objectives:
Hemorrhagic
transformation
after
endovascular
thrombectomy
predicts
poor
outcomes
in
acute
ischemic
stroke
with
large-vessel
occlusion.
The
roles
of
microRNAs
(miRNAs)
the
pathogenesis
parenchymal
hematoma
(PH)
still
remain
unclear.
This
study
aimed
to
investigate
miRNA
and
mRNA
regulatory
network
associated
PH
mechanical
reperfusion
an
animal
model
oxygen-glucose
deprivation/reoxygenation
(OGD/R)
model.
Methods:
Twenty-five
miRNAs
were
assessed
a
reperfusion-induced
hemorrhage
rats
under
hyperglycemic
conditions
receiving
5
h
middle
cerebral
artery
differentially
expressed
neuron,
astrocyte,
microglia,
brain
microvascular
endothelial
cell
(BMEC),
pericyte
OGD/R.
predicted
target
genes
further
miRNA-mRNA
was
established.
Results:
Thirteen
down-regulated
(miRNA-29a-5p,
miRNA-29c-3p,
miRNA-126a-5p,
miRNA-132-3p,
miRNA-136-3p,
miRNA-142-3p,
miRNA-153-5p,
miRNA-218a-5p,
miRNA-219a-2-3p,
miRNA-369-5p,
miRNA-376a-5p,
miRNA-376b-5p,
miRNA-383-5p)
one
up-regulated
(miRNA-195-3p)
found
rat
peri-infarct
reperfusion.
Of
these
14
PH-related
miRNAs,
10
significantly
at
least
two
five
BMEC,
models
OGD/R,
consistent
results.
Thirty-one
hub
Forty-nine
axes
revealed,
they
related
mechanisms
inflammation,
immunity,
oxidative
stress,
apoptosis.
Conclusions:
Fourteen
OGD/R
models.
Simultaneously
several
cells
neurovascular
unit
may
serve
as
valuable
targets
for
stroke.
Molecular Neurobiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 12, 2025
This
study
investigated
the
role
of
integrin-linked
kinase
(ILK)
in
neuronal
apoptosis
induced
by
cerebral
ischemia‒reperfusion
injury
(CIRI)
and
its
interaction
with
a
circRNA
(0000964)
miR-758-3p.
Using
vivo
vitro
rat
models,
we
clarified
how
ILK
regulates
during
CIRI.
Our
findings
revealed
that
expression
is
upregulated
response
to
CIRI
modulated
(0000964)/miR-758-3p
axis.
provides
new
insights
into
molecular
mechanisms
suggests
potential
therapeutic
targets
reduce
apoptosis.
A
model
was
created
through
middle
artery
occlusion
(MCAO).
After
miR-758-3p
overexpression,
neurological
deficits,
volume,
levels
circRNAs
were
evaluated.
Neurons
subjected
oxygen‒glucose
deprivation
(OGD)
simulate
CIRI,
same
molecules
analyzed.
MCAO-induced
downregulated
Similarly,
OGD-induced
Further
analysis
confirmed
negatively
miR-758-3p,
which
turn
ILK.
axis
controls
Caspase-3
expression,
influencing
has
been
identified
as
key
regulator
The
modulates
ILK,
impacting
survival.
network
offers
pathophysiology
highlights
possible
approaches.
Neurology International,
Journal Year:
2025,
Volume and Issue:
17(4), P. 55 - 55
Published: April 9, 2025
Background/Objectives:
Ischemic
stroke
remains
a
leading
cause
of
death
and
disability
worldwide.
Despite
significant
progress
in
reperfusion
therapy,
the
optimal
ischemic
management
strategy
has
not
been
developed.
Recent
studies
demonstrate
that
microRNA
may
play
an
essential
role
pathophysiology
its
possible
potential
to
be
treatment
target
point.
The
proposed
systematic
review
aimed
report
relationship
between
IS's
clinical
severity
miRNA
expression.
Secondary
outcomes
included
infarct
volume,
systemic
inflammatory
markers,
prognosis,
as
well
additional
features
such
subtype,
comorbidity,
risk
subsequent
correlation
Methods:
We
have
performed
search
database
resources
according
PRISMA
statement
guidelines.
Twenty-seven
on
total
number
3906
patients
were
assessed
suitable
for
present
SR.
Included
analyzed
expression
30
different
fragments.
Results:
After
investigating
available
data,
we
identified
set
fragment
candidates
used
diagnostics
base
development
future
protocols.
Conclusions:
Studies
presented
SR
indicate
significantly
associated
with
severity,
inflammation
stroke.
More
prospective,
properly
designed
protocols
consistent
methods
testing
optimized
assessment
are
needed
confirm
course
