Dynamic equilibrium of skeletal muscle macrophage ontogeny in the diaphragm during homeostasis, injury, and recovery DOI Creative Commons
Qian Li, Feng‐Xia Liang, Salyan Bhattarai

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 21, 2024

The diaphragm is a unique skeletal muscle due to its continuous activation pattern during the act of breathing. ontogeny macrophages, pivotal cells for maintenance and regeneration, primarily based on two distinct origins: postnatal bone marrow-derived monocytes prenatal embryonic progenitors. Here we employed chimeric mice study dynamics these macrophage populations under different conditions. Traditional generated through whole body irradiation showed virtually complete elimination original tissue-resident pool. We then developed novel method which employs lead shielding protect tissue niche from irradiation. This allowed us determine that up almost half macrophages in can be maintained independently steady-state These findings were confirmed by long-term (5 months) parabiosis experiments. Acute injury shifted balance toward an overwhelming predominance marrow (monocyte)-derived macrophages. However, there was remarkable reversion pre-injury ontological landscape after recovery. permits analysis origin corresponding function physiological pathological It may especially useful studying diseases are characterized acute or chronic accompanying inflammation.

Language: Английский

Metabolism and bioenergetics in the pathophysiology of organ fibrosis DOI
Verónica Miguel, Elena Alcalde‐Estévez,

Belén Sirera

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 222, P. 85 - 105

Published: June 4, 2024

Language: Английский

Citations

3
Unveiling the Intricate Dance: How Cancer Orchestrates Muscle Wasting and Sarcopenia DOI Open Access
Salvatore Lavalle, Maria Rosaria Valerio,

Edoardo Masiello

et al.

In Vivo, Journal Year: 2024, Volume and Issue: 38(4), P. 1520 - 1529

Published: Jan. 1, 2024

Sarcopenia is a prevalent and clinically significant condition, particularly among older age groups those with chronic disease. Patients cancer frequently suffer from sarcopenia progressive loss of muscle mass, strength, function. The complex interplay between its treatment, including medical therapy, radiotherapy, surgery, significantly contributes to the onset worsening sarcopenia. Cancer induces wasting through inflammatory processes, metabolic alterations, hormonal imbalance. Moreover, radiation therapies exert direct toxic effects on muscles, contributing impairment physical Loss appetite, malnutrition, inactivity further exacerbate in patients. Imaging techniques are cornerstones for diagnosis. Magnetic resonance imaging, computed tomography, dual-energy X-ray absorptiometry provide valuable insights into structure quality. Although each modality has advantages limitations, magnetic imaging produces high-resolution images provides dynamic information about Despite these challenges, addressing essential optimizing treatment outcomes improving survival rates patients cancer. This review explored factors oncologic patients, emphasizing importance early detection comprehensive management strategies.

Language: Английский

Citations

3
Radiation induces long‐term muscle fibrosis and promotes a fibrotic phenotype in fibro‐adipogenic progenitors DOI Creative Commons
Nicolás Collao,

Donna M. D’Souza,

Laura Messeiller

et al.

Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2023, Volume and Issue: 14(5), P. 2335 - 2349

Published: Sept. 6, 2023

Radiation-induced muscle pathology, characterized by atrophy and fibrotic tissue accumulation, is the most common debilitating late effect of therapeutic radiation exposure particularly in juvenile cancer survivors. In healthy muscle, fibro/adipogenic progenitors (FAPs) are required for maintenance regeneration, while pathology FAPs precursors exacerbated extracellular matrix deposition. However, role radiation-induced has not previously been explored.Four-week-old Male CBA or C57Bl/6J mice received a single dose (16 Gy) irradiation (IR) to hindlimb with shielded contralateral limb (CLTR) serving as non-IR control. Mice were sacrificed 3, 7, 14 (acute IR response), 56 days post-IR (long-term response). Changes skeletal morphology, myofibre composition, niche cellular dynamics, DNA damage, proliferation, mitochondrial respiration, metabolism changes progenitor cell fate where assessed.Juvenile resulted smaller cross-sectional area, type I IIA myofibres (P < 0.05) reduced proportion 0.05). Skeletal fibrosis was evident at post-IR. The IR-limb had fewer endothelial cells fibro-adipogenic Fewer satellite (stem) detected 3 induced FAP senescence 0.05), increased their fibrogenic differentiation 0.01), promoted glycolytic metabolism. Further, altered secretome manner that impaired fusion 0.05).Our study suggests following exposure, contribute long-term fibrosis. These findings provide rationale investigating FAP-targeted therapies ameliorate negative effects muscle.

Language: Английский

Citations

6
Point of Care Design and Manufacturing Accelerates Innovation Translation for Patients with Head and Neck Cancer DOI
Emma Charters, Shaheen Hasmat, Kai Cheng

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Obesity Promotes Marrow-Derived Myeloid Cell Accumulation while Exercise Reduces Proliferative Signaling in Colon Cancer DOI
James J. Vanhie,

Lisa Ek Orloff,

Alice Tate

et al.

Medicine & Science in Sports & Exercise, Journal Year: 2024, Volume and Issue: 57(2), P. 317 - 326

Published: Oct. 1, 2024

ABSTRACT Purpose Obesity increases colon cancer risk that has been previously linked to marrow-derived myeloid cells. We demonstrated exercise training (EX) prevents initiation, potentially through reduced myelopoiesis. However, it remains unknown whether early cell accumulation and inflammation in the precedes carcinogenesis with high-fat diet (HFD)-induced obesity, if EX can attenuate these effects. hypothesized obesity would promote was preceded by be attenuated EX. Methods C57BL/6 mice were randomized a HFD or control (CON) for 8 weeks. The switched CON all given intraperitoneal injections of azoxymethane (AOM) induce into sedentary (SED) conditions. Results developed more aberrant crypt foci (ACF), marker carcinogenesis, compared ( P < 0.01), fewer ACF SED 0.0001). Marrow-derived 0.001) CD206 + macrophages elevated at study week 16 0.01). − 0.05) abundant 42. did not alter immune populations. β-catenin protein higher 42 0.05), STAT3 content lower 28 0.05). Conclusions results suggest promotes formation, inflammatory accumulation. Despite attenuating ACF, colon, suggesting inhibits formation alternative mechanisms which may include signaling.

Language: Английский

Citations

0
Dynamic equilibrium of skeletal muscle macrophage ontogeny in the diaphragm during homeostasis, injury, and recovery DOI Creative Commons
Qian Li, Feng‐Xia Liang, Salyan Bhattarai

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 21, 2024

The diaphragm is a unique skeletal muscle due to its continuous activation pattern during the act of breathing. ontogeny macrophages, pivotal cells for maintenance and regeneration, primarily based on two distinct origins: postnatal bone marrow-derived monocytes prenatal embryonic progenitors. Here we employed chimeric mice study dynamics these macrophage populations under different conditions. Traditional generated through whole body irradiation showed virtually complete elimination original tissue-resident pool. We then developed novel method which employs lead shielding protect tissue niche from irradiation. This allowed us determine that up almost half macrophages in can be maintained independently steady-state These findings were confirmed by long-term (5 months) parabiosis experiments. Acute injury shifted balance toward an overwhelming predominance marrow (monocyte)-derived macrophages. However, there was remarkable reversion pre-injury ontological landscape after recovery. permits analysis origin corresponding function physiological pathological It may especially useful studying diseases are characterized acute or chronic accompanying inflammation.

Language: Английский

Citations

0