Applications of scanning electron microscopy in taxonomy with special reference to family Euphorbiaceae

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 83(9), P. 1066 - 1078

Published: June 9, 2020

Abstract The present study was carried out to identify the 20 medicinally important species of family Euphorbiaceae by aid scanning electron microscopy (SEM) foliar anatomical characteristics. Both qualitative and quantitative measurements for characters like epidermal cells, stomata, trichomes, subsidiary cells on both abaxial(ab) adaxial(ad) epidermis were recorded. Remarkable variations in these features had been observed among studied species. Most irregular or polygonal shape, only five hexagonal that is, Euphorbia neriifolia L., prostate Aiton, Jatropha integerrima Jacq., Vernicia fordii (Hemsl.), royleana Boiss. Stomata abundant abaxial as compared adaxial epidermis. E. pulcherrima Willd. Ex Klotzsch Phyllanthus emblica L. possessed anomocytic helioscopia cotinifolia Ricinus communis anisocytic while rest paracytic stomata. Trichomes very few including hirta Klotzsch, Putranjiva roxburghii Wall. Similarly, also reported such can be distinguished from basis length 103.4 ± 0.15 74.9 0.55 μm, respectively. Moreover, trichomes with 408 μm P. trichome 314.2 1.35 These findings confirmed taxonomic utility traits identification taxa.

Language: Английский

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Seed morphometry and ultrastructure studies on some Turkish orchids (Orchidaceae)

Microscopy Research and Technique, Journal Year: 2021, Volume and Issue: 84(10), P. 2409 - 2420

Published: April 27, 2021

Abstract Orchid seeds have great morphological variations that imply the phylogenetic relationship of species depending on biodiversity family or act as an adaptation to seed dispersal mechanisms life form. This study aims both describe and analyse qualitative quantitative traits 12 Turkish orchids representing epidendroids orchidoids in detail investigate which properties are diagnostic among these taxa also reveal if differentiated ecological preferences studied species. Both features were determined, measurements obtained using light scanning electron microscopy. We applied unweighted pair group method with arithmetic mean (UPGMA) cluster analysis canonical discriminant traits. Furthermore, we analyzed same orchid correlation such habitats elevation confirmed usefulness data sets for effectively assessing variation seeds. Although characters cell shape differences chalazal medial region, sizes, numbers longitudinal axis, periclinal wall ornamentation taxonomically conserved, some other characteristics shape, absence ornamentation, larger embryo size developmental achievement germination. confirms value features, effective explaining variety.

Language: Английский

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Comparative authentication of Semiliquidambar cathayensis and its substituted species via macroscopic and microscopic features

Chinese Herbal Medicines, Journal Year: 2022, Volume and Issue: 14(4), P. 535 - 542

Published: Sept. 1, 2022

Ban Fenghe recorded in the Quality Standard of Yao Medicine Guangxi Zhuang Autonomous Region (Volume 1) is derived from dried stems and leaves Semiliquidambar cathayensis. It usually confused with medicinal herbs Pterospermum heterophyllum Dendropanax dentiger. However, they are very different chemical composition, should not be used as same drug. To ensure their safety efficacy, a method based on macroscopic microscopic characteristics was developed to distinguish them.A total 14 batches samples three species were collected producing areas China. The examined by observing external traits. tissue structures transverse sections leaves, leaf epidermis, powder observed microscopically.The branchlets surfaces S. cathayensis P. hairy, especially lower densely covered hairs, but those D. dentiger hairless. pericyclic fibers intermittently distributed circular shape accompanied stone cells, whereas bundled without cells. So cells hairs present powder, found powder. distribution sites, sizes types secretory tissues these also leaves. Stomata epidermis paracytic, anomocytic.Ban drugs could readily distinguished features.

Language: Английский

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Micromorphology of the leaf surface in some species of Dryadoideae (Rosaceae)

Micron, Journal Year: 2023, Volume and Issue: 167, P. 103428 - 103428

Published: Feb. 10, 2023

Language: Английский

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Scanning electron microscopic screening; Can it be a taxonomic tool for identification of traditional therapeutic plants

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 84(4), P. 730 - 745

Published: Oct. 30, 2020

Abstract The limiting factor in the utilization of herbal medicine is risk replacement or substitution their plant resources. Therefore, authentication medicinal plants before its use medicines essentially required. Hence, prime objective this study was to provide some reliable morpho‐anatomical and morpho‐palynological tools for identification nine traditional therapeutic district Lahore. For study, scanning electron microscopic light approaches has been employed. Results reported great variations qualitative quantitative features epidermal cell, stomatal complexes, trichomes, silica bodies oil droplets. example, Aegle marmelos L. can be distinguished from Cestrum diurnum based on cell shapes, as irregular cells former lobed shape later one. Similarly unicellular, bicellular, multicellular trichomes could an basis Cynodon dactylon L., Lantana camara Pongamia pinnata respectively. In combination with these characters, SEM‐based characterization also diagnostic value. Both possessed similar (irregular) but delimited pollen morphology spheroidal whereas prolate . Moreover, characters aid well species delimitation, such found both Terminalia chebula Retz. varied P/E ratio, that is, 0.97 0.88, leaf correlation morph‐palynological traits showed potential taxonomic resolution at level. Research Highlights This provides valuable district, Lahore, Pakistan. Light studies provided useful systematic data studied plants. include moprho‐anatomical keys used future purposes.

Language: Английский

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