Journal of Controlled Release, Journal Year: 2022, Volume and Issue: 345, P. 832 - 850
Published: March 31, 2022
Language: Английский
Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(2)
Published: Feb. 1, 2024
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical applications due challenges nomenclature, separation non-vesicular extracellular particles, characterisation functional studies. To address opportunities this rapidly evolving field, International Society for Vesicles (ISEV) updates its 'Minimal Information Studies Vesicles', which was first published 2014 then 2018 MISEV2014 MISEV2018, respectively. The goal current document, MISEV2023, is provide researchers with an updated snapshot available approaches advantages limitations production, multiple sources, including culture, body fluids solid tissues. In addition presenting latest art principles research, document also covers advanced techniques that are currently expanding boundaries field. MISEV2023 includes new sections on release uptake a brief discussion vivo study Compiling feedback ISEV expert task forces more than 1000 researchers, conveys research facilitate robust discoveries move field forward even rapidly.
Language: Английский
Citations
1384
Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(5), P. 369 - 382
Published: March 8, 2022
Language: Английский
Citations
810
Journal of Extracellular Vesicles, Journal Year: 2021, Volume and Issue: 10(11)
Published: Sept. 1, 2021
Abstract In this study we tested whether a protein corona is formed around extracellular vesicles (EVs) in blood plasma. We isolated medium‐sized nascent EVs of THP1 cells as well Optiprep‐purified platelets, and incubated them EV‐depleted plasma from healthy subjects patients with rheumatoid arthritis. were subjected to differential centrifugation, size exclusion chromatography, or density gradient ultracentrifugation followed by mass spectrometry. Plasma protein‐coated had higher compared the ones carried numerous newly associated proteins. Interactions between proteins confirmed confocal microscopy, capillary Western immunoassay, immune electron microscopy flow cytometry. identified nine shared EV (ApoA1, ApoB, ApoC3, ApoE, complement factors 3 4B, fibrinogen α‐chain, immunoglobulin heavy constant γ2 γ4 chains), which appear be common among EVs, viruses artificial nanoparticles An unexpected finding was high overlap composition aggregates. This explained our that besides diffuse, patchy corona, large aggregates also associate surface EVs. However, while an external cargo induced increased expression TNF‐α, IL‐6, CD83, CD86 HLA‐DR human monocyte‐derived dendritic cells, EV‐free no effect. conclusion, data may shed new light on origin commonly reported ‘contamination’ preparations add perspective research.
Language: Английский
Citations
315
Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(6)
Published: June 1, 2022
Abstract Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological processes hold great potential for therapeutic diagnostic use. Despite significant advances within the last decade, issue of EV storage stability remains unresolved under investigated. Here, we aimed to identify conditions stabilizing EVs comprehensively compared impact various buffer formulations at different temperatures on derived from cellular sources up 2 years. features including concentration, diameter, surface protein profile nucleic acid contents were assessed by complementary methods, engineered containing fluorophores or functionalized proteins utilized compare uptake ligand binding. We show that storing PBS over time leads drastically reduced recovery particularly pure samples all tested, starting already days. further report using as diluent was found result severely rates minutes. Several tested new largely prevented observed effects, lead candidate being supplemented with human albumin trehalose (PBS‐HAT). PBS‐HAT facilitates clearly improved short‐term long‐term preservation stored ‐80°C, throughout several freeze‐thaw cycles, when downstream applications.
Language: Английский
Citations
248
Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(4)
Published: April 1, 2022
Abstract Nanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell‐derived extracellular vesicles (EVs). Here we demonstrate that nano‐sized EVs from therapy‐grade human placental‐expanded (PLX) stromal cells are surrounded by an imageable and functional when enriched with permissive technology. Scalable EV separation cell‐secreted soluble factors via tangential flow‐filtration (TFF) subtractive tandem mass‐tag (TMT) proteomics revealed significant enrichment of predominantly immunomodulatory proangiogenic proteins. Western blot, calcein‐based flow cytometry, super‐resolution electron microscopy verified PLX‐EVs partly protected proteins protease digestion. significantly ameliorated skin regeneration angiogenesis in vivo, induced differential signalling immune cells, dose‐dependently inhibited T cell proliferation vitro. removal size‐exclusion or ultracentrifugation abrogated angiogenesis. Re‐establishing artificial cloaking fluorescent albumin as model defined was depicted microscopy, zeta‐potential shift, served proof‐of‐concept. Understanding formation will improve rational EV‐inspired nano‐therapy design.
Language: Английский
Citations
170
Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(10)
Published: Oct. 1, 2022
Extracellular vesicles (EVs) are lipid-membrane enclosed nanoparticles that play significant roles in health and disease. EVs abundant body fluids carry an array of molecules (proteins, lipids, nucleic acids glycans) reflect the identity activity their cell-of-origin. While advent high throughput omics technologies has allowed in-depth characterisation EV compositions, how these molecular species spatially distributed within structures is not well appreciated. This particularly true surface where a plethora reported to be both integral peripherally associated membrane. coronal layer or 'atmosphere' surrounds membrane contributes large, highly interactive dynamic area responsible for facilitating interactions with extracellular environment. The harbours parent cells, which likely valuable property context diagnostic liquid biopsies. In this review, we describe current understanding mechanical, electrostatic properties offer biomarker potential contribute interactome.
Language: Английский
Citations
122
Military Medical Research, Journal Year: 2023, Volume and Issue: 10(1)
Published: Aug. 17, 2023
Abstract Skin wounds are characterized by injury to the skin due trauma, tearing, cuts, or contusions. As such injuries common all human groups, they may at times represent a serious socioeconomic burden. Currently, increasing numbers of studies have focused on role mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in wound repair. cell-free therapy, MSC-derived EVs shown significant application potential field repair as more stable and safer option than conventional therapy. Treatment based can significantly promote damaged substructures, including regeneration vessels, nerves, hair follicles. In addition, inhibit scar formation affecting angiogenesis-related antifibrotic pathways promoting macrophage polarization, angiogenesis, proliferation, migration, inhibiting excessive matrix production. Additionally, these structures serve scaffold for components used repair, be developed into bioengineered support trauma Through formulation standardized culture, isolation, purification, drug delivery strategies, exploration detailed mechanism will allow them clinical treatments conclusion, EVs-based therapies important prospects Here we provide comprehensive overview their current status, potential, associated drawbacks.
Language: Английский
Citations
98
Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)
Published: July 27, 2023
Language: Английский
Citations
89
Acta Pharmacologica Sinica, Journal Year: 2022, Volume and Issue: 43(11), P. 2759 - 2776
Published: April 4, 2022
Language: Английский
Citations
88
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7986 - 7986
Published: July 20, 2022
Extracellular vesicles (EVs) are minute with lipid bilayer membranes. EVs secreted by cells for intercellular communication. Recently, have received much attention, as they rich in biological components such nucleic acids, lipids, and proteins that play essential roles tissue regeneration disease modification. In addition, can be developed vaccines against cancer infectious diseases, the vesicle membrane has an abundance of antigenic determinants virulent factors. therapeutic applications typically collected from conditioned media cultured cells. However, number is limited. Thus, it critical to devise new strategies large-scale production EVs. Here, we discussed utilized researchers scalable Techniques bioreactors, mechanical stimulation, electrical thermal magnetic field topographic clue, hypoxia, serum deprivation, pH modification, exposure small molecules, nanoparticles, increasing intracellular calcium concentration, genetic modification been used improve secretion nitrogen cavitation, porous extrusion, sonication prepare EV-mimetic nanovesicles share many characteristics naturally Apart inducing EV production, these upscaling interventions also reported modify EVs' cargo thus their functionality potential. summary, imperative identify a reliable technique produce large quantities consistently. Ideally, produced should possess improved
Language: Английский
Citations
79