Trends in biotechnology, Journal Year: 2023, Volume and Issue: 41(7), P. 965 - 981
Published: Feb. 6, 2023
Language: Английский
Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(2)
Published: Feb. 1, 2024
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical applications due challenges nomenclature, separation non-vesicular extracellular particles, characterisation functional studies. To address opportunities this rapidly evolving field, International Society for Vesicles (ISEV) updates its 'Minimal Information Studies Vesicles', which was first published 2014 then 2018 MISEV2014 MISEV2018, respectively. The goal current document, MISEV2023, is provide researchers with an updated snapshot available approaches advantages limitations production, multiple sources, including culture, body fluids solid tissues. In addition presenting latest art principles research, document also covers advanced techniques that are currently expanding boundaries field. MISEV2023 includes new sections on release uptake a brief discussion vivo study Compiling feedback ISEV expert task forces more than 1000 researchers, conveys research facilitate robust discoveries move field forward even rapidly.
Language: Английский
Citations
1384
Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(4)
Published: April 1, 2022
Abstract Nanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell‐derived extracellular vesicles (EVs). Here we demonstrate that nano‐sized EVs from therapy‐grade human placental‐expanded (PLX) stromal cells are surrounded by an imageable and functional when enriched with permissive technology. Scalable EV separation cell‐secreted soluble factors via tangential flow‐filtration (TFF) subtractive tandem mass‐tag (TMT) proteomics revealed significant enrichment of predominantly immunomodulatory proangiogenic proteins. Western blot, calcein‐based flow cytometry, super‐resolution electron microscopy verified PLX‐EVs partly protected proteins protease digestion. significantly ameliorated skin regeneration angiogenesis in vivo, induced differential signalling immune cells, dose‐dependently inhibited T cell proliferation vitro. removal size‐exclusion or ultracentrifugation abrogated angiogenesis. Re‐establishing artificial cloaking fluorescent albumin as model defined was depicted microscopy, zeta‐potential shift, served proof‐of‐concept. Understanding formation will improve rational EV‐inspired nano‐therapy design.
Language: Английский
Citations
170
Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(9)
Published: Sept. 1, 2022
Small extracellular vesicles (sEVs) provide major promise for advances in cancer diagnostics, prognostics, and therapeutics, ascribed to their distinctive cargo reflective of pathophysiological status, active involvement intercellular communication, as well ubiquity stability bodily fluids. As a result, the field sEV research has expanded exponentially. Nevertheless, there is lack standardisation methods isolation from cells grown serum-containing media. The majority researchers use media harvest employ ultracentrifugation primary method. Ultracentrifugation inefficient it devoid capacity isolate high yields without contamination non-sEV materials or disruption integrity. We comprehensively evaluated protocol using tangential flow filtration size exclusion chromatography sEVs variety human murine cell lines, including HeLa, MDA-MB-231, EO771 B16F10. directly compared performance traditional methods, that had undergone further purification by chromatography, separate sEVs, rigorously characterised properties multiple quantification devices, protein analyses both image nano-flow cytometry. enrich consistent populations, with similar distributions particles ranging up 200 nm. However, exceeds isolating significantly higher making more suitable large-scale applications. Our results demonstrate reliable robust approach surpasses yield, reproducibility, time, costs scalability. These advantages allow implementation comprehensive applications downstream investigations.
Language: Английский
Citations
120
Military Medical Research, Journal Year: 2023, Volume and Issue: 10(1)
Published: Aug. 17, 2023
Abstract Skin wounds are characterized by injury to the skin due trauma, tearing, cuts, or contusions. As such injuries common all human groups, they may at times represent a serious socioeconomic burden. Currently, increasing numbers of studies have focused on role mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in wound repair. cell-free therapy, MSC-derived EVs shown significant application potential field repair as more stable and safer option than conventional therapy. Treatment based can significantly promote damaged substructures, including regeneration vessels, nerves, hair follicles. In addition, inhibit scar formation affecting angiogenesis-related antifibrotic pathways promoting macrophage polarization, angiogenesis, proliferation, migration, inhibiting excessive matrix production. Additionally, these structures serve scaffold for components used repair, be developed into bioengineered support trauma Through formulation standardized culture, isolation, purification, drug delivery strategies, exploration detailed mechanism will allow them clinical treatments conclusion, EVs-based therapies important prospects Here we provide comprehensive overview their current status, potential, associated drawbacks.
Language: Английский
Citations
101
Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)
Published: July 27, 2023
Language: Английский
Citations
89
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7986 - 7986
Published: July 20, 2022
Extracellular vesicles (EVs) are minute with lipid bilayer membranes. EVs secreted by cells for intercellular communication. Recently, have received much attention, as they rich in biological components such nucleic acids, lipids, and proteins that play essential roles tissue regeneration disease modification. In addition, can be developed vaccines against cancer infectious diseases, the vesicle membrane has an abundance of antigenic determinants virulent factors. therapeutic applications typically collected from conditioned media cultured cells. However, number is limited. Thus, it critical to devise new strategies large-scale production EVs. Here, we discussed utilized researchers scalable Techniques bioreactors, mechanical stimulation, electrical thermal magnetic field topographic clue, hypoxia, serum deprivation, pH modification, exposure small molecules, nanoparticles, increasing intracellular calcium concentration, genetic modification been used improve secretion nitrogen cavitation, porous extrusion, sonication prepare EV-mimetic nanovesicles share many characteristics naturally Apart inducing EV production, these upscaling interventions also reported modify EVs' cargo thus their functionality potential. summary, imperative identify a reliable technique produce large quantities consistently. Ideally, produced should possess improved
Language: Английский
Citations
79
Nature Cell Biology, Journal Year: 2022, Volume and Issue: 24(9), P. 1322 - 1325
Published: Aug. 30, 2022
Language: Английский
Citations
75
Nature Nanotechnology, Journal Year: 2024, Volume and Issue: 19(6), P. 846 - 855
Published: Feb. 16, 2024
Abstract Extracellular vesicles (EVs) derived from mesenchymal stem cells are promising nanotherapeutics in liver diseases due to their regenerative and immunomodulatory properties. Nevertheless, a concern has been raised regarding the rapid clearance of exogenous EVs by phagocytic cells. Here we explore impact protein corona on two culturing conditions which specific proteins acquired media were simultaneously adsorbed EV surface. Additionally, incubating with serum, simulating formation upon systemic delivery, further resolved corona–EV complex patterns investigated. Our findings reveal potential influences composition under vitro vivo kinetics. data suggest that bound albumin creates an signature can retarget hepatic macrophages. This results markedly improved cellular uptake hepatocytes, sinusoidal endothelial stellate phenomenon be applied as camouflage strategy precoating fabricate albumin-enriched complex, enhancing non-phagocytic liver. work addresses critical challenge facing intravenously administered for therapy tailoring cell targeting immune evasion.
Language: Английский
Citations
65
Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)
Published: March 27, 2023
Recent advances in extracellular vesicle (EVs) detection and isolation methods have led to the development of novel therapeutic modalities. Among different types EVs, exosomes (Exos) can transfer signaling biomolecules exhibit several superior features compared whole-cell-based therapies. Therapeutic factors are normally loaded into Exo lumen or attached their surface for improving on-target delivery rate regenerative outcomes. Despite these advantages, there limitations application Exos vivo conditions. It was suggested that a set proteins other biological compounds adsorbed around aqueous phases constitute an external layer named protein corona (PC). Studies shown PC affect physicochemical properties synthetic natural nanoparticles (NPs) after introduction biofluids. Likewise, is generated especially This review article preliminary attempt address interfering effects on bioactivity effects. Video Abstract.
Language: Английский
Citations
64
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(33)
Published: June 27, 2024
Extracellular vesicles (EVs) are promising next-generation therapeutics and drug delivery systems due to demonstrated safety efficacy in preclinical models early-stage clinical trials. There is an urgent need address the immunogenicity of EVs (beyond apparent lack immunotoxicity) advance development. To date, few studies have assessed unintended immunological recognition EVs. An in-depth understanding EV-induced clearance necessary develop effective therapeutic strategies, including approaches mitigate when undesired. This article summarizes various factors involved potential strategies reduce for improved benefit.
Language: Английский
Citations
56