Unraveling multilayered extracellular vesicles: Speculation on cause DOI
Kelly Broad, Sierra A. Walker,

Irina Davidovich

et al.

Journal of Extracellular Vesicles, Journal Year: 2023, Volume and Issue: 12(2)

Published: Feb. 1, 2023

Extracellular vesicles (EVs) are cell-released, heterogenous nanoparticles that play important roles in (patho)physiological processes through intercellular communication. EVs often depicted as having a single lipid bilayer, but many studies have demonstrated the existence of multilayered EVs. There has been minimal inquiry into differences between unilamellar and multilamellar terms biogenesis mechanisms functional effects. This commentary speculates on potential causes serves call to action for research community unravel complex layers

Language: Английский

Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications DOI Creative Commons
Liwei Wang, Di Wang, Zhaoming Ye

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)

Published: April 20, 2023

Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.

Language: Английский

Citations

62
Intervertebral disc degeneration—Current therapeutic options and challenges DOI Creative Commons

Ankita Samanta,

Thomas Lufkin, Petra Kraus

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: July 6, 2023

Degeneration of the intervertebral disc (IVD) is a normal part aging. Due to spine's declining function and development pain, it may affect one's physical health, mental socioeconomic status. Most degeneration (IVDD) therapies today focus on symptoms low back pain rather than underlying etiology or mechanical disc. The deteriorated typically not restored by conservative surgical that largely correcting structural abnormalities. To enhance clinical outcome quality life patient, several therapeutic modalities have been created. In this review, we discuss genetic environmental causes IVDD describe promising modern endogenous exogenous approaches including their applicability relevance process.

Language: Английский

Citations

55
MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research DOI Creative Commons
Fabrice Lucien, Dakota Gustafson, Metka Lenassi

et al.

Journal of Extracellular Vesicles, Journal Year: 2023, Volume and Issue: 12(12)

Published: Dec. 1, 2023

Abstract Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma serum) are not only donor‐dependent but also influenced by collection preparation protocols. Since there hundreds pre‐analytical protocols over forty variables, development standard operating procedures EV research very challenging. To improve reproducibility research, International Society Extracellular Vesicles (ISEV) Task Force proposes standardized reporting (i) applied protocol (ii) quality prepared serum samples. Gathering detailed information will provide insight into performance more effectively identify potential confounders in collect this information, ISEV created Minimal Information (MIBlood‐EV), tool to record report about as well assays assess these preparations. This does require modifications established local can be easily implemented enhance existing databases thereby enabling evidence‐based optimization through meta‐analysis. Taken together, samples biobanks guide exchange between laboratories, (iii) facilitate inter‐laboratory comparative studies, (iv) peer review process.

Language: Английский

Citations

51
Towards artificial intelligence-enabled extracellular vesicle precision drug delivery DOI Creative Commons
Zachary Greenberg, Kiley Graim, Mei He

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 199, P. 114974 - 114974

Published: June 24, 2023

Language: Английский

Citations

45
Plant-Derived Exosome-like Nanoparticles for Biomedical Applications and Regenerative Therapy DOI Creative Commons
Andari Sarasati, Muhammad Hidayat Syahruddin, Archadian Nuryanti

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1053 - 1053

Published: March 29, 2023

Plant-derived exosome-like nanoparticles (PDENs) comprise various bioactive biomolecules. As an alternative cell-free therapeutic approach, they have the potential to deliver nano-bioactive compounds human body, and thus lead anti-inflammatory, antioxidant, anti-tumor benefits. Moreover, it is known that Indonesia one of herbal centers world, with abundance unexplored sources PDENs. This encouraged further research in biomedical science develop natural richness plants as a source for welfare. study aims verify PDENs purposes, especially regenerative therapy applications, by collecting analyzing data from latest relevant developments.

Language: Английский

Citations

44
Extracellular vesicles as human therapeutics: A scoping review of the literature DOI Creative Commons
Clorinda Fusco,

Giusy De Rosa,

Ilaria Spatocco

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(5)

Published: May 1, 2024

Abstract Extracellular vesicles (EVs) are released by all cells and contribute to cell‐to‐cell communication. The capacity of EVs target specific efficiently deliver a composite profile functional molecules have led researchers around the world hypothesize their potential as therapeutics. While studies EV treatment in animal models numerous, actual clinical benefit humans has more slowly started be tested. In this scoping review, we searched PubMed other databases up 31 December 2023 and, starting from 13,567 records, selected 40 pertinent published testing therapeutics humans. analysis those shows that they small pilot trials with large heterogeneity terms administration route disease. Moreover, absence placebo control most studies, predominant local application formulations inconsistent dose metric still impede comparison across firm conclusions about safety efficacy. On hand, recording some promising outcomes strongly calls out for well‐designed larger test an alternative approach treat human diseases no or few therapeutic options.

Language: Английский

Citations

34
A critical systematic review of extracellular vesicle clinical trials DOI Creative Commons
Rachel R. Mizenko,

Madison Feaver,

Batuhan Turhan Bozkurt

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(10)

Published: Sept. 27, 2024

Abstract This systematic review examines the landscape of extracellular vesicle (EV)‐related clinical trials to elucidate field's trends in applications and EV‐related methodologies, with an additional focus on acknowledgement EV subpopulations. By analysing data from public reporting repositories, we catalogued 471 date, indications for over 200 diseases. Diagnostics companion diagnostics represented bulk cancer being most frequent application. therapeutics mainly utilized mesenchymal stromal cell (MSC) EVs were frequently used treatment respiratory illnesses. Ultracentrifugation RNA‐sequencing common isolation characterization techniques; however, methodology each was not reported study records. Most relied isolates, only 11% utilizing subpopulations their experimental design. While this may be connected a lack available techniques suitable implementation, it also highlights opportunity use improve translational efforts. As academic research identifies more chemically distinct technologies enrichment, forecast refined near future. emphasizes need meticulous methodological consideration enhance success EV‐based interventions, pointing towards paradigm shift personalized medicine.

Language: Английский

Citations

24
Insight into the Functional Dynamics and Challenges of Exosomes in Pharmaceutical Innovation and Precision Medicine DOI Creative Commons
Anu Sharma, Anita Yadav,

Aparajita Nandy

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(6), P. 709 - 709

Published: May 24, 2024

Of all the numerous nanosized extracellular vesicles released by a cell, endosomal-originated exosomes are increasingly recognized as potential therapeutics, owing to their inherent stability, low immunogenicity, and targeted delivery capabilities. This review critically evaluates transformative of exosome-based modalities across pharmaceutical precision medicine landscapes. Because precise biomolecular cargo delivery, posited ideal candidates in drug enhancing regenerative strategies, advancing diagnostic technologies. Despite significant market growth projections exosome therapy, its utilization is encumbered substantial scientific regulatory challenges. These include lack universally accepted protocols for isolation complexities associated with navigating environment, particularly guidelines set forth U.S. Food Drug Administration (FDA). presents comprehensive overview current research trajectories aimed at addressing these impediments discusses prospective advancements that could substantiate clinical translation exosomal therapies. By providing analysis both capabilities hurdles therapeutic applications, this article aims inform direct future paradigms, thereby fostering integration systems into mainstream practice.

Language: Английский

Citations

17
Amniotic fluid stem cell extracellular vesicles as a novel fetal therapy for pulmonary hypoplasia: a review on mechanisms and translational potential DOI Creative Commons
Fabian Doktor, Lina Antounians, Rebeca Lopes Figueira

et al.

Stem Cells Translational Medicine, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 1, 2025

Disruption of developmental processes affecting the fetal lung leads to pulmonary hypoplasia. Pulmonary hypoplasia results from several conditions including congenital diaphragmatic hernia (CDH) and oligohydramnios. Both entities have high morbidity mortality, no effective therapy that fully restores normal development. Hypoplastic lungs impaired growth (arrested branching morphogenesis), maturation (decreased epithelial/mesenchymal differentiation), vascularization (endothelial dysfunction vascular remodeling leading postnatal hypertension). Herein, we discuss pathogenesis role microRNAs (miRNAs) during pathological Since multiple cells pathways are altered, ideal strategy for hypoplastic is deliver a addresses all aspects abnormal In this review, report on novel regenerative approach based administration extracellular vesicles derived amniotic fluid stem (AFSC-EVs). Specifically, describe effects AFSC-EVs in rodent human models hypoplasia, their mechanism action via release cargo, miRNAs, anti-inflammatory properties. We also compare cargo contents EVs AFSCs mesenchymal stromal (MSCs). Overall, there compelling evidence antenatal rescues features development experimental Lastly, steps need be taken translate promising EV-based bench bedside. These include strategies overcome barriers commonly associated with EV therapeutics specific challenges related cell-based therapies medicine.

Language: Английский

Citations

2
Impact of Storage Conditions on EV Integrity/Surface Markers and Cargos DOI Creative Commons
Ayyanar Sivanantham, Yang Jin

Life, Journal Year: 2022, Volume and Issue: 12(5), P. 697 - 697

Published: May 7, 2022

Extracellular vesicles (EVs) are small biological particles released into biofluids by every cell. Based on their size, they classified EVs (<100 nm or <200 nm) and medium large (>200 nm). In recent years, have garnered interest for potential medical applications, including disease diagnosis, cell-based biotherapies, targeted drug delivery systems, others. Currently, the long-term short-term storage temperatures −80 °C 4 °C, respectively. The capacity of can depend number, function, temperature, duration, freeze−thaw cycles. While these parameters increasingly studied, effects preservation conditions integrity remain to be understood. Knowledge gaps in areas may ultimately impede widespread applicability EVs. Therefore, this review summarizes current knowledge effect stability critically explores prospective ways improving ensure EV stability.

Language: Английский

Citations

65