Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(30)
Published: June 14, 2024
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
Applied Materials Today,
Journal Year:
2024,
Volume and Issue:
36, P. 102061 - 102061
Published: Jan. 16, 2024
Formulation
development
is
a
critical
step
in
the
of
medicines.
The
process
requires
human
creativity,
ingenuity
and
in-depth
knowledge
formulation
processing
optimization,
which
can
be
time-consuming.
Herein,
we
tested
ability
artificial
intelligence
(AI)
to
create
de
novo
formulations
for
three-dimensional
(3D)
printing.
Specifically,
conditional
generative
adversarial
networks
(cGANs),
are
models
known
their
were
trained
on
dataset
consisting
1437
fused
deposition
modelling
(FDM)
printed
that
extracted
from
both
literature
in-house
data.
In
total,
27
different
cGANs
architectures
explored
with
varying
learning
rate,
batch
size
number
hidden
layers
parameters
generate
270
formulations.
After
comparison
between
characteristics
AI-generated
human-generated
formulations,
it
was
discovered
medium
rate
(10−4)
could
strike
balance
generating
novel
realistic.
Four
these
fabricated
using
an
FDM
printer,
first
successfully
printed.
Our
study
represents
milestone,
highlighting
capacity
AI
undertake
creative
tasks
its
potential
revolutionize
drug
process.
Cell Communication and Signaling,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Feb. 11, 2025
Extracellular
vesicles
(EVs)
are
relevant
elements
for
cell-to-cell
communication
and
considered
crucial
in
host-pathogen
interactions
by
transferring
molecules
between
the
pathogen
host
during
infections.
These
structures
participate
various
physiological
pathological
processes
promising
candidates
as
disease
markers,
therapeutic
reagents,
drug
carriers.
Both
H.
pylori
epithelial
cells
infected
secrete
EVs,
which
contribute
to
inflammation
development
of
phenotypes.
However,
many
aspects
cellular
molecular
biology
EV
functions
remain
incompletely
understood
due
methodological
challenges
studying
these
small
structures.
This
review
also
highlights
roles
EVs
derived
from
pylori-infected
pathogenesis
gastric
extragastric
diseases.
Understanding
specific
infections,
whether
advantageous
or
pathogen,
may
help
new
approaches
prevent
disease.
Stem Cell Research & Therapy,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Feb. 12, 2025
Bone-related
diseases
impact
a
large
portion
of
the
global
population
and,
due
to
their
high
disability
rates
and
limited
treatment
options,
pose
significant
medical
economic
challenges.
Mesenchymal
stem
cells
(MSCs)
can
differentiate
into
multiple
cell
types
offer
strong
regenerative
potential,
making
them
promising
for
treating
various
diseases.
However,
issues
with
immune
response
survival
limit
effectiveness
transplantation.
This
has
led
increased
interest
in
cell-free
therapy,
particularly
use
exosomes,
which
is
most
studied
form
this
approach.
Exosomes
are
extracellular
vesicles
that
contain
proteins,
lipids,
nucleic
acids
play
key
role
communication
material
exchange.
Pyroptosis,
death
involved
innate
immunity,
also
associated
many
Studies
have
shown
MSC-derived
exosomes
therapeutic
potential
range
conditions
by
regulating
inflammation
pyroptosis.
study
explored
modulating
pyroptosis
improve
bone-related
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
Extracellular
vesicles
(EVs)
are
critical
mediators
of
intercellular
communication,
carrying
bioactive
cargo
and
displaying
diverse
surface
components
that
reflect
their
cellular
origins
functions.
The
EV
surface,
composed
proteins,
lipids,
glycocalyx
elements,
plays
a
pivotal
role
in
targeting
recipient
cells,
mediating
biological
interactions,
enabling
selective
delivery.
This
review
comprehensively
examined
the
molecular
architecture
surfaces,
linking
biogenesis
to
functional
diversity,
highlights
therapeutic
diagnostic
potential
diseases
such
as
cancer
cardiovascular
disorders.
Additionally,
we
explore
emerging
applications
EVs,
including
machine-learning-assisted
analysis,
chemical
integration,
cross-system
combinations.
also
discusses
some
key
challenges
clinical
translation
EV-related
technologies.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(20), P. 15206 - 15206
Published: Oct. 15, 2023
Extracellular
vesicles
(EVs)
are
small,
membrane-bound
used
by
cells
to
deliver
biological
cargo
such
as
proteins,
mRNA,
and
other
biomolecules
from
one
cell
another,
thus
inducing
a
specific
response
in
the
target
powerful
method
of
organ
communication,
especially
during
pathogenesis
human
disease.
Thus,
EVs
may
be
utilized
prognostic
diagnostic
biomarkers,
but
they
also
hold
therapeutic
potential
just
mesenchymal
stem
have
been
therapeutics.
However,
unmodified
exhibit
poor
targeting
efficacy,
leading
necessity
engineered
EVS.
To
highlight
advantages
promises
EVs,
this
review,
we
summarized
research
progress
on
past
ten
years,
five
highlighted
their
applications
development
for
diseases.
Compared
existing
cell-derived
EV-based
strategies,
show
greater
promise
clinical
applications:
First,
mediate
good
efficacy
exhibiting
peptide
that
allows
them
specifically
or
even
type,
avoiding
accumulation
undesired
locations
increasing
potency
treatment.
Second,
can
artificially
pre-loaded
with
any
necessary
biomolecular
drugs
treat
variety
diseases
cancers,
neurological
diseases,
cardiovascular
ailments.
Further
is
improve
logistical
challenges
large-scale
EV
manufacturing,
current
developments
prove
promising
greatly
treatment
traditionally
difficult
