Journal of Neuroinflammation,
Journal Year:
2023,
Volume and Issue:
20(1)
Published: Jan. 31, 2023
Abstract
Chronic
microglia
activation
post-stroke
is
associated
with
worse
neurological
and
cognitive
outcomes.
However,
measurement
of
in
vivo
currently
limited.
Plasma
derived
extracellular
vesicles
(EVs)
are
cell-specific
indicators
that
may
allow
for
non-invasive
phenotype.
The
aim
this
study
was
to
identify
activation-state
specific
EVs
(MEVs)
vitro
followed
by
validation
an
experimental
stroke
model.
Following
pro-inflammatory
activation,
MEVs
contain
the
protein
TMEM119
alongside
increased
expression
Toll-like
receptor
4
co-receptor
CD14.
Immunoprecipitation
fluorescent
nanoparticle
tracking
analysis
(ONI
Nanoimager)
used
confirm
isolation
+
/CD14
from
rat
plasma.
Electron
microscopy
confirmed
CD14
localize
MEV
membrane.
To
model
ischemia,
plasma
collected
3-month
wildtype
Fischer344
rats
prior
to,
7
28
days
after
endothelin-1
or
saline
injection
into
dorsal
right
striatum.
Fluorescently
labelled
were
directly
measured
using
nanoflow
cytometry
(Apogee
A60
Microplus).
We
report
a
significant
increase
circulating
28-days
comparison
baseline
levels
saline-injected
rats,
which
correlated
weakly
volume.
/MHC-II
also
animals.
This
first
describe
EV
biomarker
activated
detected
following
represents
future
tool
activity
.
European Journal of Cell Biology,
Journal Year:
2024,
Volume and Issue:
103(3), P. 151441 - 151441
Published: July 6, 2024
Integrins
are
heterodimeric
membrane
proteins
expressed
on
the
surface
of
most
cells.
They
mediate
adhesion
and
signaling
processes
relevant
for
a
wealth
physiological
processes,
including
nervous
system
development
function.
Interestingly,
integrins
also
recognized
therapeutic
targets
inflammatory
diseases,
such
as
multiple
sclerosis.
Here,
we
discuss
role
in
brain
function,
well
neurodegenerative
diseases
affecting
(Alzheimer's
disease,
sclerosis,
stroke).
Furthermore,
targeting
these
receptors
brain.
Frontiers in Cellular Neuroscience,
Journal Year:
2022,
Volume and Issue:
16
Published: Sept. 13, 2022
As
resident
component
of
the
innate
immunity
in
central
nervous
system
(CNS),
microglia
are
key
players
pathology.
However,
they
also
exert
fundamental
roles
brain
development
and
homeostasis
maintenance.
They
extremely
sensitive
plastic,
as
assiduously
monitor
environment,
adapting
their
function
response
to
stimuli.
On
consequence,
may
be
defined
a
heterogeneous
community
cells
dynamic
equilibrium.
Extracellular
vesicles
(EVs)
released
by
mirror
nature
donor
cells,
exerting
important
versatile
functions
CNS
unbounded
conveyors
bioactive
signals.
In
this
review,
we
summarize
current
knowledge
on
EVs
microglia,
highlighting
properties
multifaceted
effects.
Alzheimer s & Dementia,
Journal Year:
2023,
Volume and Issue:
19(12), P. 5418 - 5436
Published: May 19, 2023
Abstract
INTRODUCTION
Extracellular
vesicles
(EVs)
have
been
implicated
in
the
spread
of
neuropathology
Alzheimer's
disease
(AD),
but
their
involvement
behavioral
outcomes
linked
to
AD
remains
be
determined.
METHODS
EVs
isolated
from
post
mortem
brain
tissue
control,
AD,
or
frontotemporal
dementia
(FTD)
donors,
as
well
APP/PS1
mice,
were
injected
into
hippocampi
wild‐type
(WT)
a
humanized
Tau
mouse
model
(hTau/mTauKO).
Memory
tests
carried
out.
Differentially
expressed
proteins
assessed
by
proteomics.
RESULTS
Both
AD‐EVs
and
APP/PS1‐EVs
trigger
memory
impairment
WT
mice.
We
further
demonstrate
that
FTD‐EVs
carry
protein,
present
altered
protein
composition
associated
with
synapse
regulation
transmission,
hTau/mTauKO
DISCUSSION
Results
negative
impacts
on
mice
suggest
that,
addition
spreading
pathology,
may
contribute
FTD.
Highlights
Aβ
was
detected
enriched
PSP
FTD
tissue.
AD‐derived
induce
cognitive
AD‐
FTD‐derived
Proteomics
findings
associate
dysregulation
tauopathies.
Journal of Neuroinflammation,
Journal Year:
2023,
Volume and Issue:
20(1)
Published: Jan. 31, 2023
Abstract
Chronic
microglia
activation
post-stroke
is
associated
with
worse
neurological
and
cognitive
outcomes.
However,
measurement
of
in
vivo
currently
limited.
Plasma
derived
extracellular
vesicles
(EVs)
are
cell-specific
indicators
that
may
allow
for
non-invasive
phenotype.
The
aim
this
study
was
to
identify
activation-state
specific
EVs
(MEVs)
vitro
followed
by
validation
an
experimental
stroke
model.
Following
pro-inflammatory
activation,
MEVs
contain
the
protein
TMEM119
alongside
increased
expression
Toll-like
receptor
4
co-receptor
CD14.
Immunoprecipitation
fluorescent
nanoparticle
tracking
analysis
(ONI
Nanoimager)
used
confirm
isolation
+
/CD14
from
rat
plasma.
Electron
microscopy
confirmed
CD14
localize
MEV
membrane.
To
model
ischemia,
plasma
collected
3-month
wildtype
Fischer344
rats
prior
to,
7
28
days
after
endothelin-1
or
saline
injection
into
dorsal
right
striatum.
Fluorescently
labelled
were
directly
measured
using
nanoflow
cytometry
(Apogee
A60
Microplus).
We
report
a
significant
increase
circulating
28-days
comparison
baseline
levels
saline-injected
rats,
which
correlated
weakly
volume.
/MHC-II
also
animals.
This
first
describe
EV
biomarker
activated
detected
following
represents
future
tool
activity
.
