Isolation and Characterization of Cetacean Cell-Derived Extracellular Vesicles DOI Creative Commons
Valentina Moccia, Cinzia Centelleghe, Ilaria Giusti

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(21), P. 3304 - 3304

Published: Oct. 24, 2023

Cetaceans are of scientific interest because they good candidates as environmental bioindicators. However, in vivo research is arduous and vitro studies represent a rarely used valid alternative. Extracellular vesicles (EVs) membrane-bound structures playing roles cell-to-cell communication. Despite being promising investigative tool different fields science, EVs have been poorly studied cetaceans. To fill this gap, we describe the preliminary characterization isolated from bottlenose dolphin Cuvier’s beaked whale cell line. with ultracentrifugation (UC) or size exclusion chromatography (SEC) characterized nanoparticle tracking analysis (NTA), Western blotting (WB), scanning transmission electron microscopy (STEM). UC SEC allowed isolation mainly small (<200 nm). A higher number particles were through compared to both lines. At WB, all expressed EV-markers CD9 integrin-β. Only positive for TSG101. In conclusion, first time line using two techniques. Further on cell-derived will be useful deepen our knowledge cetacean pathophysiology health status assessment.

Language: Английский

Plant-Derived Exosome-Like Nanovesicles: Current Progress and Prospects DOI Creative Commons
Nai Mu, Jie Li, Li Zeng

et al.

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 4987 - 5009

Published: Sept. 1, 2023

Abstract: Exosomes are small extracellular vesicles, ranging in size from 30– 150nm, which can be derived various types of cells. In recent years, mammalian-derived exosomes have been extensively studied and found to play a crucial role regulating intercellular communication, thereby influencing the development progression numerous diseases. Traditional Chinese medicine has employed plant-based remedies for thousands an increasing body evidence suggests that plant-derived exosome-like nanovesicles (PELNs) share similarities with terms their structure function. this review, we provide overview advances study PELNs potential implications human health. Specifically, summarize roles respiratory, digestive, circulatory, other Furthermore, investigated shortcomings challenges current research regarding mechanism action, safety, administration routes, isolation extraction methods, characterization identification techniques, as well drug-loading capabilities. Based on these considerations, propose recommendations future directions. Overall, our review highlights promising area research, broad treatment We anticipate continued interest hope summary findings will stimulate further exploration into Keywords: exosomes, nanocarriers, nanotherapeutics, plants, vesicles

Language: Английский

Citations

75
Plant-derived extracellular vesicles (PDEVs) in nanomedicine for human disease and therapeutic modalities DOI Creative Commons
Zhijie Xu, Yuzhen Xu, Kui Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: March 29, 2023

Abstract Background The past few years have witnessed a significant increase in research related to plant-derived extracellular vesicles (PDEVs) biological and medical applications. Using biochemical technologies, multiple independent groups demonstrated the important roles of PDEVs as potential mediators involved cell-cell communication exchange bio-information between species. Recently, several contents been well identified PDEVs, including nucleic acids, proteins, lipids, other active substances. These cargoes carried by could be transferred into recipient cells remarkably influence their behaviors associated with human diseases, such cancers inflammatory diseases. Main body abstract This review summarizes latest updates regarding focuses on its role nanomedicine applications, drug delivery strategies develop diagnostic therapeutic agents for clinical management especially like cancers. Conclusion Considering unique advantages, high stability, intrinsic bioactivity easy absorption, further elaboration molecular mechanisms factors driving function will provide new horizons treatment disease.

Language: Английский

Citations

46
Bioactive compounds and biological functions of medicinal plant-derived extracellular vesicles DOI Creative Commons
Siyu Hao,

Hongyu Yang,

Jiaojiao Hu

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 200, P. 107062 - 107062

Published: Jan. 9, 2024

Extracellular vesicles (EVs) are tiny lipid bilayer-enclosed membrane particles released from a variety of cell types into the surrounding environment. These EVs have massive participated in cell-to-cell communication and interspecies communication. In recent years, plant-derived extracellular (PDEVs) "exosome-like" populations found distinct plants attracted widespread attention. Especially, research on medicinal (MPDEVs) increasing, which considered kind promising natural compound. This review summarizes current knowledge MPDEVs terms bioactive compounds, including small RNA, protein, lipid, metabolite, been surface and/or lumen MPDEVs. Moreover, both vitro vivo experiments shown that exert broad biomedical functions, such as anti-inflammatory, anticancer, antioxidant, modulate microbiota, etc. may be better substitute than animal-derived (ADEVs) because safety biocompatibility future.

Language: Английский

Citations

17
An in vitro approach to understand contribution of kidney cells to human urinary extracellular vesicles DOI Creative Commons
Karina Barreiro, Abigail C. Lay,

Germán Leparc

et al.

Journal of Extracellular Vesicles, Journal Year: 2023, Volume and Issue: 12(2)

Published: Feb. 1, 2023

Abstract Extracellular vesicles (EV) are membranous particles secreted by all cells and found in body fluids. Established EV contents include a variety of RNA species, proteins, lipids metabolites that considered to reflect the physiological status their parental cells. However, date, little is known about cell‐type enriched cargo complex mixtures, especially urine. To test whether secretion from distinct human kidney culture differ can recapitulate findings normal urine, we comprehensively analysed components, (particularly miRNAs, long RNAs protein) conditionally immortalised cell lines (podocyte, glomerular endothelial, mesangial proximal tubular cells) compared media derived were isolated hydrostatic filtration dialysis (HFD) characterised electron microscopy (EM), nanoparticle tracking analysis (NTA) Western blotting (WB). was subjected miRNA sequencing proteins profiled tandem mass tag proteomics. Representative sets detected each type urinary isolates (uEV), database, biopsy bulk proteomics, single‐cell transcriptomics. This revealed high proportion vitro signatures also vivo datasets. Thus, highlighting robustness our model showing this approach enables dissection specific biofluids potential identification biomarkers disease.

Language: Английский

Citations

19
Engineered plant extracellular vesicles for autoimmune diseases therapy DOI

Ruina Han,

Yan Wu, Yafei Han

et al.

Nano Research, Journal Year: 2023, Volume and Issue: 17(4), P. 2857 - 2873

Published: Sept. 18, 2023

Language: Английский

Citations

16
The compound XueShuanTong promotes podocyte mitochondrial autophagy via the AMPK/mTOR pathway to alleviate diabetic nephropathy injury DOI

Chuangbiao Zhang,

Weiwei Ren, Xiaohua Lü

et al.

Mitochondrion, Journal Year: 2025, Volume and Issue: unknown, P. 102024 - 102024

Published: March 1, 2025

Language: Английский

Citations

0
Unraveling Extracellular Vesicle DNA: Biogenesis, Functions, and Clinical Implications DOI

Mehraneh Nouri,

F Nasiri,

Samaneh Sharif

et al.

Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: unknown, P. 155937 - 155937

Published: March 1, 2025

Language: Английский

Citations

0
FLT1-enriched extracellular vesicles induce a positive feedback loop between nasopharyngeal carcinoma cells and endothelial cells to promote angiogenesis and tumour metastasis DOI
Fei Li, Song Lin, Yue He

et al.

Oncogene, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

Language: Английский

Citations

0
Extracellular vesicles: From large-scale production and engineering to clinical applications DOI Creative Commons

Yuxuan Wang,

Jiali Xiong,

Kun Ouyang

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Extracellular vesicles (EVs) have emerged as a promising strategy for treating wide spectrum of pathologies, they can deliver their cargo to recipient cells and regulate the signaling pathway these modulate fate. Despite great potential EVs in clinical applications, low yield challenges loading remain significant obstacles, hindering transition from experimental research practice. Therefore, promoting EV release enhancing cargo-loading are fields with substantial broad application prospects. In this review, we summarize applications EVs, methods technologies large-scale production, engineering, modification, well that must be addressed during development. We also discuss future perspectives exciting field facilitate its transformation bench reality.

Language: Английский

Citations

0
Exploring the role of urinary extracellular vesicles in kidney physiology, aging, and disease progression DOI Creative Commons
Cristina Grange,

A P Dalmasso,

Judiel John Cortez

et al.

AJP Cell Physiology, Journal Year: 2023, Volume and Issue: 325(6), P. C1439 - C1450

Published: Oct. 16, 2023

Extracellular vesicles (EVs), membranous present in all body fluids, are considered important messengers, carrying their information over long distance and modulating the gene expression profile of recipient cells. EVs collected urine (uEVs) mainly originated from apical part urogenital tract, following flow. Moreover, bacterial-derived within may reflect composition microbiota. Consolidated evidence has established involvement uEVs renal physiology, being responsible for glomerular tubular cross talk among different segments. also be involved other physiological functions such as modulation innate immunity, coagulation, or metabolic activities. Furthermore, it been recently remonstrated that age, sex, endurance excise, lifestyle influence uEV release, modifying cargo. On hand, appear modulators pathological conditions, triggering disease progression. sustain fibrosis inflammation processes, both acute chronic kidney diseases, aging, stone formation. The molecular signature diseased patients can interest understanding physiopathology identifying diagnostic prognostic biomarkers.

Language: Английский

Citations

9