Gold nanomaterials capped with bovine serum albumin for cell and extracellular vesicle imaging DOI
Re‐Wen Wu, Yu‐Han Lin, Cheng-Hsiu Lu

et al.

Nanotechnology, Journal Year: 2024, Volume and Issue: 36(10), P. 105101 - 105101

Published: Dec. 30, 2024

Abstract Bovine serum albumin-capped gold nanoclusters (AuNC@BSA) are ionic, ultra-small, and eco-friendly nanomaterials that exhibit red fluorescence emission. Upon modification, these can serve as imaging probes with multimodal functionality. Owing to their nanoscale properties, AuNC@BSA-based be readily endocytosed by cells for imaging. With the increasing interest in cell therapy, extracellular vesicles (EVs) have attracted considerable attention from researchers; however, effective methods EVs remain limited. Although several studies explored strategies using compounds, nuclear pharmaceuticals, nanoparticles, or genetic constructs, use of labeling parental has rarely been discussed, even less paid potential. To address this gap, we utilized three types derivatives: AuNC@BSA, AuNC@BSA-Gd, AuNC@BSA-Gd-I. Our findings demonstrate derivatives effectively label both a simple direct approach, which is particularly notable EVs, they typically require more complex procedures. Furthermore, potential labeled was evaluated, revealing capabilities In summary, study presents an strategy nanomaterials. These will contribute accelerating development drug delivery systems, cell- EV-based therapies, advanced strategies.

Language: Английский

Inter‐laboratory multiplex bead‐based surface protein profiling of MSC‐derived EV preparations identifies MSC‐EV surface marker signatures DOI Creative Commons

Vivian V. T. Nguyen,

Joshua A Welsh, Tobias Tertel

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(6)

Published: June 1, 2024

Abstract Mesenchymal stromal cells (MSCs) are promising regenerative therapeutics that primarily exert their effects through secreted extracellular vesicles (EVs). These EVs – being small and non‐living easier to handle possess advantages over cellular products. Consequently, the therapeutic potential of MSC‐EVs is increasingly investigated. However, due variations in MSC‐EV manufacturing strategies, products should be considered as highly diverse. Moreover, diverse array EV characterisation technologies used for further complicates reliable interlaboratory comparisons published data. this study aimed establish a common method can easily by various researchers characterise preparations facilitate comparisons. To end, we conducted comprehensive inter‐laboratory assessment using novel multiplex bead‐based flow cytometry assay panel. This involved 11 different from five laboratories with varying MSC sources, culture conditions, preparation methods. Through panel covering range mostly MSC‐related markers, identified set cell surface markers consistently positive (CD44, CD73 CD105) or negative (CD11b, CD45 CD197) on all explored preparations. Hierarchical clustering analysis revealed distinct marker profiles associated specific processes laboratory conditions. We propose CD73, CD105 CD44 robust minimally identifying MSC‐derived CD11b, CD14, CD19, CD79 markers. Additionally, highlight influence medium components, particularly human platelet lysate, profiles, underscoring conditions resulting standardisable approach profiling offers tool routine manufactured pre‐clinical clinical research, enhances quality control preparations, hopefully paves way higher consistency reproducibility emerging field.

Language: Английский

Citations

15
Surface Components and Biological Interactions of Extracellular Vesicles DOI
Jinping Wang, Kuoran Xing, Guo-Ying Zhang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins functions. The EV surface, composed proteins, lipids, glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, enabling selective delivery. This review comprehensively examined the molecular architecture surfaces, linking biogenesis to functional diversity, highlights therapeutic diagnostic potential diseases such as cancer cardiovascular disorders. Additionally, we explore emerging applications EVs, including machine-learning-assisted analysis, chemical integration, cross-system combinations. also discusses some key challenges clinical translation EV-related technologies.

Language: Английский

Citations

1
Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profiling DOI
Solène Lenoir, Elliot Thouvenot,

Giacomo Gropplero

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

ABSTRACT This study presents a novel method and device for the hydrodynamic production of extracellular vesicles (EVs) derived from biomimetic multicellular 3D spheroids, enabling high-throughput particle release that is 10 to 20 times higher than in non-stimulated conditions. The facilitates formation spheroids human mesenchymal stem cells (hMSCs), offering an all-in-one approach both spheroid generation EV release. Production are reduced just few hours, with yield further increased by alternating periods high flow recovery sequential approach. Using this system, we explored impact starvation conditions on protein cargo EVs, identifying distinct markers through proteomics. Specifically, stimulation enriched EVs plasma membrane-derived mitochondrial proteins, revealing divergent biogenesis pathways. Importantly, produced exhibited therapeutic properties, demonstrated effects wound healing, angiogenesis, anti-inflammatory responses, some showing enhanced efficacy under stimulation.

Language: Английский

Citations

0
Identification of robust and abundant reference transcripts for EV mRNA cargo normalization DOI
Antje M. Zickler, Radosław Grochowski, André Görgens

et al.

Extracellular Vesicle, Journal Year: 2025, Volume and Issue: 5, P. 100065 - 100065

Published: Jan. 22, 2025

Language: Английский

Citations

0
Therapeutic targets in aging-related osteoarthritis: A focus on the extracellular matrix homeostasis DOI Creative Commons
Hao Wan, Ming‐Fu Chang, Di Shi

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123487 - 123487

Published: Feb. 1, 2025

Language: Английский

Citations

0
Mesenchymal stem cell exosome therapy: current research status in the treatment of neurodegenerative diseases and the possibility of reversing normal brain aging DOI Creative Commons

Junjie Quan,

Qing Liu,

Pinghui Li

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 21, 2025

With the exacerbation of aging population trend, a series neurodegenerative diseases caused by brain have become increasingly common, significantly impacting daily lives elderly and imposing heavier burdens on nations societies. Brain is complex process involving multiple mechanisms, including oxidative stress, apoptosis damaged neuronal cells, chronic inflammation, mitochondrial dysfunction, research into new therapeutic strategies to delay has gradually focus in recent years. Mesenchymal stem cells (MSCs) been widely used cell therapy due their functions such as antioxidative anti-inflammation, tissue regeneration. However, accompanying safety issues immune rejection, tumor development, pulmonary embolism cannot be avoided. Studies shown that using exosome derived from mesenchymal (MSC-Exo) for treatment safe effective method. It not only effects but also avoids risks associated with therapy. Therefore, exploring normal mechanism MSC-Exo feasible. This review summarizes characteristics clinical progress diseases, aiming explore possibility potential mechanisms reversing aging.

Language: Английский

Citations

0
Multi-omics analysis of uterine fluid extracellular vesicles reveals a resemblance with endometrial tissue across the menstrual cycle: biological and translational insights DOI Creative Commons
Apostol Apostolov, Danilo Mladenović,

Kadi Tilk

et al.

Human Reproduction Open, Journal Year: 2025, Volume and Issue: 2025(2)

Published: Jan. 1, 2025

Abstract STUDY QUESTION Does the molecular composition of uterine fluid extracellular vesicles (UF-EVs) reflect endometrial tissue changes across menstrual cycle? SUMMARY ANSWER Concordance between and UF-EVs exists on miRNA mRNA levels along cycle phases UF-EV surface proteomic signatures suggest EVs originate from several major cell populations. WHAT IS KNOWN ALREADY The clinical value receptivity testing is restricted by invasiveness use only one omics level input. There promising evidence that can in mid-secretory endometrium, highlighting potential to establish right before embryo transfer. However, dynamic cargo have not been directly compared multiple levels. DESIGN, SIZE, DURATION This cross-sectional study included fertile women four phases: proliferative early-, mid-, late-secretory phases. In total, 26 paired samples UF were collected. sequenced, differential analysis was performed consecutive profiled for various protein markers associated with different types. epithelial organoid-conditioned culture media used as a reference pure EVs. PARTICIPANTS/MATERIALS, SETTING, METHODS Paired collected fertile, reproductive-age women. EV isolation validated using electron microscopy western blotting, particle numbers measured nanoparticle tracking analysis. transcriptome miRNome expression conducted cycle. Bead-based flow cytometry targeting 37 characterize organoids. MAIN RESULTS AND THE ROLE OF CHANCE Surface proteome revealed phase had significantly increased natural killer marker CD56 (P < 0.005), pan-leukocyte CD45 pan-T-cell CD3 coagulation-related CD142 0.005) those phase, whereas cells (CD29, CD133, CD326) did change Transcriptomic highlighted histone metallothionein genes correlated tissues each tested phase. Principal component miRNomes resulted similar clustering patterns, where mid- clustered closely, early-secretory separately. Half differentially expressed miRNAs also endometrium. Importantly, nine DE identified, five which common biopsies, including hsa-miR-30d-5p hsa-miR-200b-3p, both previously implicated implantation. Notably, three miRNAs, hsa-miR-141-3p, hsa-miR-200a-3p, predicted regulate mRNAs pre-implantation trophectoderm. LARGE SCALE DATA N/A LIMITATIONS, REASONS FOR CAUTION dating based first day menstruation time LH peak, does exclude possibility expected reached. wider limitation our lack standardized procedures collecting gynaecological practice, could challenge replication findings. WIDER IMPLICATIONS FINDINGS Evidence supports testing. Additionally, further studies pathologies be beneficial diagnostics, considering more invasive biopsies biopsy site full FUNDING/COMPETING INTEREST(S) supported European Regional Development Fund Enterprise Estonia’s Applied Research Program under grant agreement number 2014-2020.4.02.21-0398 (EVREM), Estonian Council (grant nos. PRG1076 PSG1082), Horizon Europe NESTOR no. 101120075) Commission, Swedish 2024-02530), Novo Nordisk Fonden NNF24OC0092384), National Recovery Resilience Plan Republic Bulgaria, project BG-RRP-2.004-0001-C01. A.S.L. received funding Becas Fundación Ramón Areces para Estudios Postdoctorales. All authors declare no conflict interest.

Language: Английский

Citations

0
Recent Advances in Mass Spectrometry-Based Bottom-Up Proteomics DOI
Cameron S. Movassaghi, Jie Sun, Yuming Jiang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Mass spectrometry-based proteomics is about 35 years old, and recent progress appears to be speeding up across all subfields. In this review, we focus on advances over the last two in select areas within bottom-up proteomics, including approaches high-throughput experiments, data analysis using machine learning, drug discovery, glycoproteomics, extracellular vesicle structural proteomics.

Language: Английский

Citations

0
Mesenchymal Stromal Cell-Derived Extracellular Vesicles in the Management of Atopic Dermatitis: A Scoping Review of Therapeutic Opportunities and Challenges DOI Creative Commons
Maimonah Eissa Al-Masawa, Loai A. Elfawy, Chiew Yong Ng

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 2673 - 2693

Published: March 1, 2025

Atopic dermatitis (AD) is a global concern marked by inflammation, skin barrier dysfunction, and immune dysregulation. Current treatments primarily address symptoms without offering cure, underscoring the need for innovative therapeutic approaches. Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) have attracted attention their potential in immunomodulation tissue repair, similar to parent cells. This review provides comprehensive analysis of current landscape MSC-EV research AD management. We identified 12 studies that met our predefined inclusion criteria. thoroughly reviewed both human animal studies, analyzing aspects such as source, isolation, characterization MSC-EVs, well disease models, dosage strategies, efficacy, mechanisms, adverse effects. While this highlights promising therapy AD, it also emphasizes significant challenges, including heterogeneity insufficient reporting. Given area still its early stages, addressing these uncertainties will require collaborative efforts among researchers, regulatory bodies, international societies advance field improve patient outcomes.

Language: Английский

Citations

0
Comprehensive Comparison of Extracellular Vesicles Derived from Mesenchymal Stem Cells Cultured with Fetal Bovine Serum and Human Platelet Lysate DOI
Yue Zhang, Jing Song, Bin Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have emerged as a promising approach in regenerative therapy. However, the clinical application of MSC-EVs is hindered by presence xenogenic components, such fetal bovine serum (FBS), which most used culture supplement for MSCs. Human platelet lysate (HPL) has been proposed an alternative to FBS, but whether derived from HPL-cultured MSCs are suitable translation remains unclear. In this study, we comprehensively compared characterization EVs cultured medium with FBS (F-EVs) and HPL (H-EVs). Our study showed that promoted MSC-EV production without compromising critical quality attributes. Multiomics sequencing revealed stability H-EVs different umbilical cord donors global functional alterations under conditions. comparison F-EVs, enriched more angiogenesis-related molecules exhibited enhanced angiogenesis, were further confirmed vivo vitro studies. significantly reduced renal microvascular rarefaction regeneration vein endothelial cells hypoxia stimulation F-EVs. conclusion, our findings demonstrated supplements did not alter attributes MSC-EVs, specifically holding higher yield angiogenic potential.

Language: Английский

Citations

0