Prolidase-proline oxidase axis is engaged in apoptosis induction by birch buds flavonol santin in endometrial adenocarcinoma cell line DOI Creative Commons
Łukasz Szoka, Jolanta Nazaruk,

Joanna Giegiel

et al.

Frontiers in Molecular Biosciences, Journal Year: 2023, Volume and Issue: 10

Published: Sept. 6, 2023

Cancer of the corpus uteri and cervix uteri, collectively ranks second among new cancer cases in women after breast cancer. Therefore, investigation anticancer agents identifying molecular targets presents a challenge to improve effectiveness chemotherapy. In this study, antiproliferative activity flavonoids derived from buds silver birch downy was evaluated endometrial Ishikawa cells cervical HeLa cells. It found that flavanol santin reduced viability both cell lines better than other flavonoids, including apigenin luteolin. Moreover, slightly higher induced by chemotherapy drug, cisplatin. Santin promoted intrinsic extrinsic apoptosis pathways cells, but it had low toxicity normal fibroblasts. The mechanisms impairing included induction oxidative proline catabolism, however different ways used. increase oxidation due activation p53 leading oxidase upregulation. contrast, having basal level significantly treatment decreased its expression. Nevertheless, these since increased expression prolidase, an enzyme providing protein degradation. lines, associated with generation reactive oxygen species reduction viability. Our findings reveal involvement identify role prolidase oxidase-dependent apoptosis.

Language: Английский

Human papillomavirus is an important risk factor for esophageal carcinoma in a Chinese population DOI Creative Commons

Wen‐Jun Yuan,

Shuying Li, Jun Jia

et al.

Journal of Cancer Research and Clinical Oncology, Journal Year: 2022, Volume and Issue: 149(8), P. 5241 - 5253

Published: Nov. 17, 2022

Different types of HPV have been associated with cancer in humans, but the role esophageal (EC) is controversial. The purpose this study was to evaluate correlation between infection and EC Chinese population provide scientific basis for future prevention, control, early diagnosis, treatment strategies China.PCR detected 1112 tissue samples, 89 HPV-positive samples were by genotyping. Proximity ligation assays (PLAs) immunohistochemistry used detect expression E6 E7 proteins. Real-time fluorescent quantitative PCR integration HPV16 E6. level HPV-specific antibody IgG serum ELISA PLA.The positive rates L1, HPV16, HPV18, hpv16 + 18 hpv16/18 1,112 77.6%, 41.4%, 27.2%, 14.2% 55.4% respectively. Multiple subtypes samples. PLA showed that expressed EC109 formed complexes p53 pRb, Immunohistochemistry 56.4% 37.0%, HPV-DNA rate tissues (88.79%) higher than adjacent (54.17%). found patients a serological test.The suggests HPV, especially may be risk factor protein play key HPV-associated malignancies. These results important prevention China.

Language: Английский

Citations

4
Nouveaux marqueurs développés pour le dépistage des cancers liés aux HPV DOI Open Access
Valentine Marie Ferré, Julie Brousseau, Charlotte Charpentier

et al.

Revue Francophone des Laboratoires, Journal Year: 2024, Volume and Issue: 2024(558), P. 46 - 58

Published: Jan. 1, 2024

Citations

0
Deep feature extraction and fine κ-nearest neighbour for enhanced human papillomavirus detection in cervical cancer – a comprehensive analysis of colposcopy images DOI Creative Commons

Lipsarani Jena,

Santi Kumari Behera, Srikanta Dash

et al.

Współczesna Onkologia, Journal Year: 2024, Volume and Issue: 28(1), P. 37 - 44

Published: Jan. 1, 2024

This study introduces a novel methodology for classifying human papillomavirus (HPV) using colposcopy images, focusing on its potential in diagnosing cervical cancer, the second most prevalent malignancy among women globally. Addressing crucial gap literature, this highlights unexplored territory of HPV-based image diagnosis cancer. Emphasising suitability screening underdeveloped and low-income regions owing to small, cost-effective setup that eliminates need biopsy specimens, methodological framework includes robust dataset augmentation feature extraction EfficientNetB0 architecture.

Language: Английский

Citations

0
Prolidase-proline oxidase axis is engaged in apoptosis induction by birch buds flavonol santin in endometrial adenocarcinoma cell line DOI Creative Commons
Łukasz Szoka, Jolanta Nazaruk,

Joanna Giegiel

et al.

Frontiers in Molecular Biosciences, Journal Year: 2023, Volume and Issue: 10

Published: Sept. 6, 2023

Cancer of the corpus uteri and cervix uteri, collectively ranks second among new cancer cases in women after breast cancer. Therefore, investigation anticancer agents identifying molecular targets presents a challenge to improve effectiveness chemotherapy. In this study, antiproliferative activity flavonoids derived from buds silver birch downy was evaluated endometrial Ishikawa cells cervical HeLa cells. It found that flavanol santin reduced viability both cell lines better than other flavonoids, including apigenin luteolin. Moreover, slightly higher induced by chemotherapy drug, cisplatin. Santin promoted intrinsic extrinsic apoptosis pathways cells, but it had low toxicity normal fibroblasts. The mechanisms impairing included induction oxidative proline catabolism, however different ways used. increase oxidation due activation p53 leading oxidase upregulation. contrast, having basal level significantly treatment decreased its expression. Nevertheless, these since increased expression prolidase, an enzyme providing protein degradation. lines, associated with generation reactive oxygen species reduction viability. Our findings reveal involvement identify role prolidase oxidase-dependent apoptosis.

Language: Английский

Citations

1