A Novel High-Throughput Sample-in-Result-Out Device for the Rapid Detection of Viral Nucleic Acids DOI Creative Commons
F Wang, Fei Hu,

Yunyun Zhang

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 549 - 549

Published: Nov. 13, 2024

Clustered regularly interspaced short palindromic repeats (CRISPR) molecular diagnostic technology is one of the most reliable tools for infectious diseases due to its reaction time, high sensitivity, and excellent specificity. However, compared with fluorescent polymerase chain (PCR) technology, CRISPR lacks high-throughput automated instrumentation standardized detection reagents limiting large-scale clinical application. In this study, a device was developed by combining reagent lyophilization, extraction-free one-pot consumable system. This innovative approach enabled rapid sample-in-result-out 48 samples in 25 min demonstrated sensitivity specificity qualitative analysis samples. The obtained results show that limit designed system African swine fever virus (ASFV) 0.5 copies/μL. As proof concept, single-tube dual-target nucleic acid method developed, achieving 5 copies/μL

Language: Английский

SARS-CoV-2 Evolution: Implications for Diagnosis, Treatment, Vaccine Effectiveness and Development DOI Creative Commons
Fabrizio Angius,

Silvia Puxeddu,

S Zaimi

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 13(1), P. 17 - 17

Published: Dec. 28, 2024

The COVID-19 pandemic, driven by the rapid evolution of SARS-CoV-2 virus, presents ongoing challenges to global public health. is characterized rapidly evolving mutations, especially in (but not limited to) spike protein, complicating predictions about its evolutionary trajectory. These mutations have significantly affected transmissibility, immune evasion, and vaccine efficacy, leading multiple pandemic waves with over half a billion cases seven million deaths globally. Despite several strategies, from development administration design availability antivirals, including monoclonal antibodies, already having been employed, persistent circulation virus emergence new variants continue result high case numbers fatalities. In past four years, immense research efforts contributed much our understanding viral pathogenesis mechanism, syndrome, host-microbe interactions, effective vaccines, diagnostic tools, treatments. focus this review provide comprehensive analysis functional impact on diagnosis, treatments, effectiveness. We further discuss safety pregnancy implications hybrid immunity long-term protection against infection, as well latest developments pan-coronavirus nasal formulations, emphasizing need for continued surveillance, research, adaptive health strategies response race.

Language: Английский

Citations

4
Deciphering Host–Virus Interactions and Advancing Therapeutics for Chronic Viral Infection DOI Creative Commons
Majid Eslami,

Neda Arjmand,

Fatemeh Mahmoudian

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(3), P. 390 - 390

Published: March 10, 2025

Chronic viral infections like HIV, HBV, and HCV establish persistent interactions with the host immune system, resulting in evasion long-term dysfunction. These viruses use a range of strategies to limit defenses, such as downregulating MHC class I, disrupting interferon signaling, altering apoptosis pathways, suppressing cytotoxic T-cell activity. Key proteins, including HIV Nef, HBV X protein, NS5A, interfere antigen presentation JAK/STAT thereby reducing antiviral responses. induce exhaustion due exposure, which leads expression inhibitory receptors PD-1 CTLA-4 on T cells. Viral epigenetic changes, N6-methyladenosine modifications histone deacetylation, enhance by modulating gene infected Viruses further manipulate cytokine networks promoting an immunosuppressive environment through IL-10 TGF-β secretion, suppress inflammatory responses inhibit activation. This review examines molecular/cellular mechanisms that enable chronic escape immunity, focusing antigenic variation, disruption, control apoptotic pathways. It also addresses how genetic factors, HLA polymorphisms, influence disease progression. Lastly, we discuss host-targeted therapies, checkpoint inhibitors, treatments, CRISPR.

Language: Английский

Citations

0
Establishment of a Lateral Flow Dipstick Detection Method for Influenza A Virus Based on CRISPR/Cas12a System DOI
Xiaoyan Zhao,

Ximing Zheng,

Xiyong Yang

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(37), P. 946 - 952

Published: Jan. 1, 2024

This study aimed to develop a rapid, visual PCR-CRISPR/Cas12-LFD method for detecting influenza A by utilizing the conserved region of matrix protein gene.

Language: Английский

Citations

2
Higher Affinity Enables More Accurate Detection of SARS‐CoV‐2 in Human Saliva Using Aptamer‐based Litmus Test DOI Creative Commons
Rudi Liu, Jiuxing Li, Jimmy Gu

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(47)

Published: Aug. 12, 2024

Abstract Many aptamers have been generated by systematic evolution of ligands exponential enrichment (SELEX) to recognize spike proteins the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2&ek), some which engineered into dimeric and trimeric versions for enhanced affinity diagnostic applications. However, no studies conducted compare utilities monomeric, in assays with real clinical samples answer question what levels an aptamer must accurate diagnostics. Herein, we carried out a comparative study two monomeric MSA1 MSA5, one homotrimeric constructed varying 1000‐fold. Using colorimetric sandwich assay analyze 48 human saliva samples, found that (K d ≈10 pM) can identify SARS‐CoV‐2 infection much more accurately than ≈100 ≈10,000 pM). Based on experimental data, theoretically predict needs possess achieve 80–100 % sensitivity 100 specificity. The findings from this highlight need deriving very high enable highly detection viral future pandemics.

Language: Английский

Citations

1
The current status and future prospects of CRISPR-based detection of monkeypox virus: A review DOI
Yingwei Chen, Ran Zhao, Xiaobo Hu

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1336, P. 343295 - 343295

Published: Oct. 1, 2024

Language: Английский

Citations

1
Antibody screening-assisted multichannel nanoplasmonic sensing chip based on SERS for viral screening and variants identification DOI
Yi Liu,

Huanjiao Weng,

Zhiwei Chen

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 117015 - 117015

Published: Dec. 3, 2024

Language: Английский

Citations

1
Higher Affinity Enables More Accurate Detection of SARS‐CoV‐2 in Human Saliva Using Aptamer‐based Litmus Test DOI Creative Commons
Rudi Liu, Jiuxing Li, Jimmy Gu

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(47)

Published: Aug. 12, 2024

Abstract Many aptamers have been generated by systematic evolution of ligands exponential enrichment (SELEX) to recognize spike proteins the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2&ek), some which engineered into dimeric and trimeric versions for enhanced affinity diagnostic applications. However, no studies conducted compare utilities monomeric, in assays with real clinical samples answer question what levels an aptamer must accurate diagnostics. Herein, we carried out a comparative study two monomeric MSA1 MSA5, one homotrimeric constructed varying 1000‐fold. Using colorimetric sandwich assay analyze 48 human saliva samples, found that (K d ≈10 pM) can identify SARS‐CoV‐2 infection much more accurately than ≈100 ≈10,000 pM). Based on experimental data, theoretically predict needs possess achieve 80–100 % sensitivity 100 specificity. The findings from this highlight need deriving very high enable highly detection viral future pandemics.

Language: Английский

Citations

0
A Novel High-Throughput Sample-in-Result-Out Device for the Rapid Detection of Viral Nucleic Acids DOI Creative Commons
F Wang, Fei Hu,

Yunyun Zhang

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 549 - 549

Published: Nov. 13, 2024

Clustered regularly interspaced short palindromic repeats (CRISPR) molecular diagnostic technology is one of the most reliable tools for infectious diseases due to its reaction time, high sensitivity, and excellent specificity. However, compared with fluorescent polymerase chain (PCR) technology, CRISPR lacks high-throughput automated instrumentation standardized detection reagents limiting large-scale clinical application. In this study, a device was developed by combining reagent lyophilization, extraction-free one-pot consumable system. This innovative approach enabled rapid sample-in-result-out 48 samples in 25 min demonstrated sensitivity specificity qualitative analysis samples. The obtained results show that limit designed system African swine fever virus (ASFV) 0.5 copies/μL. As proof concept, single-tube dual-target nucleic acid method developed, achieving 5 copies/μL

Language: Английский

Citations

0