Biomimetic Proteoglycans for Intervertebral Disc (IVD) Regeneration DOI Creative Commons
Neha Chopra, James Melrose, Zi Gu

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(12), P. 722 - 722

Published: Nov. 22, 2024

Intervertebral disc degeneration, which leads to low back pain, is the most prevalent musculoskeletal condition worldwide, significantly impairing quality of life and imposing substantial socioeconomic burdens on affected individuals. A major impediment development any prospective cell-driven recovery functional properties in degenerate IVDs diminishing IVD cell numbers viability with ageing cannot sustain such a process. However, if proteoglycan levels, component, can be replenished through an orthobiological process does not rely cellular or nutritional input, then this may effective strategy for re-attainment mechanical properties. Furthermore, biomimetic proteoglycans (PGs) represent established polymer that strengthens osteoarthritis cartilage improves its biomechanical properties, actively promoting biological repair processes. Biomimetic PGs have superior water imbibing compared native aggrecan are more resistant proteolytic degradation, increasing their half-life cartilaginous tissues. Methods also now been developed chemically edit structure proteoglycans, allowing incorporation bioactive peptide modules equipping as delivery vehicles drugs growth factors, further improving biotherapeutic credentials. This article aims provide comprehensive overview strategies leverage engineered paving way novel therapeutic interventions degeneration ultimately enhancing patient outcomes.

Language: Английский

Revolutionizing the treatment of intervertebral disc degeneration: an approach based on molecular typing DOI Creative Commons
Shaofeng Chen, Wei Zhang, Yifan Liu

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 25, 2025

Intervertebral disc degeneration (IVDD) is a significant cause of global disability, reducing labor productivity, increasing the burden on public health, and affecting socio-economic well-being. Currently, there lack recognized clinical approaches for molecular classification precision therapy. Chondrocyte differentiation prognosis-related genes were extracted from single-cell RNA sequencing multi-omics data in Gene Expression Omnibus (GEO) database through chondrocyte trajectory analysis non-parametric tests. Subsequently, precise IVDD risk stratification system was developed using ConsensusClusterPlus analysis. The significance typing demonstrated case–control trials involving patients. Specific inhibitors predicted pRRophetic package R language then validated vitro. A stratified model IVDD, considering demonstrating high relevance, set 44 fate genes. Extensive analyses confirmed relevance this model, indicating that cases High Scoring Classification (HCSC) group had most favorable prognosis, whereas those Low (LCSC) worst prognosis. Additionally, studies provided evidence utility translational medicine. gene expression-based approach used to create matrix identifying potential specific each subtype. In vitro experiments revealed gefitinib, drug designed LCSC, not only protective effects chondrocytes but also could induce conversion LCSC into HCSC subgroup. Therefore, played critical role assisting clinicians with enabling personalized treatment decisions. results study have comprehensive clinically relevant offers new opportunity customizing treatments IVDD.

Language: Английский

Citations

2
Exploring the correlation between magnetic resonance diffusion tensor imaging (DTI) parameters and aquaporin expression and biochemical composition content in degenerative intervertebral disc nucleus pulposus tissue: a clinical experimental study DOI Creative Commons
Shuang Chen,

Shenglu Sun,

Nan Wang

et al.

BMC Musculoskeletal Disorders, Journal Year: 2025, Volume and Issue: 26(1)

Published: Feb. 17, 2025

This study investigates the correlation between magnetic resonance diffusion tensor imaging (DTI) parameters and biochemical composition in degenerative intervertebral disc nucleus pulposus tissue, offering a potential reference for clinical diagnosis efficacy evaluation of degeneration. Human lumbar tissue samples were collected via full endoscopic minimally invasive surgery. DTI was employed to quantitatively measure fractional anisotropy (FA) apparent coefficient (ADC) values pulposus, examining relationship Pfirrmann grading these parameters. Western blotting used detect expression levels aquaporin 1 (AQP1) 3 (AQP3) tissue. The glycosaminoglycan (GAG) content quantified using dimethylmethylene blue (DMMB) colorimetric assay, collagen assessed with Sircol soluble assay kit. also analyzed. Finally, analysis performed FA ADC from human their GAG contents. A total 39 patients (19 males, 20 females) herniation (LDH), averaging 54.41 years age, included. As degree degeneration increased, rose, while continuously declined. Concurrently, as progressed, AQP1 AQP3 proteins decreased, significantly diminished, increased. exhibited moderate negative (r = -0.5974, P < 0.0001) strong positive 0.8694, 0.0001). showed 0.6873, -0.8502, derived DTI, along protein AQPs, can reflect severity certain extent. Additionally, glycosaminoglycans correlates Therefore, quantitative provides means assess changes within discs, valuable insights therapeutic diseases. Chinese Clinical Trial Registry, ChiCTR2000041151, Registered on 19 December, 2020, https://www.chictr.org.cn/showproj.html?proj=206119 .

Language: Английский

Citations

0
Distinct role of perlecan in mesenchymal tissue regeneration via genetic and epigenetic modification DOI
Bin Wang, Zhihua Lu, Gongming Gao

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 508, P. 161103 - 161103

Published: Feb. 27, 2025

Language: Английский

Citations

0
Biomimetic Proteoglycans for Intervertebral Disc (IVD) Regeneration DOI Creative Commons
Neha Chopra, James Melrose, Zi Gu

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(12), P. 722 - 722

Published: Nov. 22, 2024

Intervertebral disc degeneration, which leads to low back pain, is the most prevalent musculoskeletal condition worldwide, significantly impairing quality of life and imposing substantial socioeconomic burdens on affected individuals. A major impediment development any prospective cell-driven recovery functional properties in degenerate IVDs diminishing IVD cell numbers viability with ageing cannot sustain such a process. However, if proteoglycan levels, component, can be replenished through an orthobiological process does not rely cellular or nutritional input, then this may effective strategy for re-attainment mechanical properties. Furthermore, biomimetic proteoglycans (PGs) represent established polymer that strengthens osteoarthritis cartilage improves its biomechanical properties, actively promoting biological repair processes. Biomimetic PGs have superior water imbibing compared native aggrecan are more resistant proteolytic degradation, increasing their half-life cartilaginous tissues. Methods also now been developed chemically edit structure proteoglycans, allowing incorporation bioactive peptide modules equipping as delivery vehicles drugs growth factors, further improving biotherapeutic credentials. This article aims provide comprehensive overview strategies leverage engineered paving way novel therapeutic interventions degeneration ultimately enhancing patient outcomes.

Language: Английский

Citations

2