Neuralized and vascularized fast bone regeneration using recombinant humanized type 1 collagen and native bone composite inorganic salts DOI Creative Commons
Peng Hou, Song Chen, Tuerxun Maimaitiaili

et al.

APL Materials, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

Bone injury is a prevalent condition in clinical therapy that can lead to significant functional impairments and substantially disrupt the quality of life for patients. However, there has been limited breakthrough achieving neuralized vascularized rapid bone regeneration. In this study, we collaborated with recombinant humanized collagen 1 (rhCOL1), native composite inorganic salts (NBCISs), methacrylated silk fibroin (SilMA), marrow mesenchymal stem cells (BMSCs) construct biomimetic organic bio-mineralized multifunctional organoids repair defects, regeneration within just six weeks rabbits. We first determined optimal concentration SilMA (10%) by comprehensively evaluating crosslinking, operability, BMSC proliferation. The rhCOL1 NBCIS mixture was prepared using ratio 3:7, reference bone, subsequently added create biomineralized microenvironments NCSilMA. Similarly, proportions were optimized based on their effects compressive modulus, swelling, degradation. As result, successfully constructed hydrogel scaffold defect repair, characterized excellent biodegradability, appropriate strength, good biocompatibility, osteoinductive biological function. Finally, BMSC-loaded NCSilMA (organoids) achieved regeneration, up-regulated osteogenic genes enhanced cell colonization, collagen, polysaccharide deposition.

Language: Английский

Bioinspired Design and Applications of Liquid Gating Gas Valve Membranes DOI Creative Commons
Yuxiu Li, Junyang Liu, Rui Xu

et al.

Biomimetics, Journal Year: 2025, Volume and Issue: 10(2), P. 77 - 77

Published: Jan. 26, 2025

In nature, dynamic liquid interfaces play a vital role in regulating gas transport, as exemplified by the adaptive mechanisms of plant stomata and liquid-lined alveoli, which enable efficient exchange through reversible opening closing. These biological processes provide profound insights into design advanced control technologies. Inspired these natural systems, gating membranes have been developed utilizing capillary-stabilized liquids to achieve precise fluid regulation. offer unique advantages rapid responses, stain resistance, high energy efficiency. Particularly, they break limitations traditional solid, porous transport. This perspective introduces bioinspired valve (LGVMs), emphasizing their opening/closing mechanism. It highlights how external stimuli can be exploited advanced, multi-level active or passive regulation strategies. Diverse applications flow selective transport are discussed. While challenges related controllability, long-term stability, scalable production persist, advancements unlock significant opportunities for groundbreaking innovations across diverse fields, including purification, microfluidics, medical diagnostics, harvesting

Language: Английский

Citations

0
Neuralized and vascularized fast bone regeneration using recombinant humanized type 1 collagen and native bone composite inorganic salts DOI Creative Commons
Peng Hou, Song Chen, Tuerxun Maimaitiaili

et al.

APL Materials, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

Bone injury is a prevalent condition in clinical therapy that can lead to significant functional impairments and substantially disrupt the quality of life for patients. However, there has been limited breakthrough achieving neuralized vascularized rapid bone regeneration. In this study, we collaborated with recombinant humanized collagen 1 (rhCOL1), native composite inorganic salts (NBCISs), methacrylated silk fibroin (SilMA), marrow mesenchymal stem cells (BMSCs) construct biomimetic organic bio-mineralized multifunctional organoids repair defects, regeneration within just six weeks rabbits. We first determined optimal concentration SilMA (10%) by comprehensively evaluating crosslinking, operability, BMSC proliferation. The rhCOL1 NBCIS mixture was prepared using ratio 3:7, reference bone, subsequently added create biomineralized microenvironments NCSilMA. Similarly, proportions were optimized based on their effects compressive modulus, swelling, degradation. As result, successfully constructed hydrogel scaffold defect repair, characterized excellent biodegradability, appropriate strength, good biocompatibility, osteoinductive biological function. Finally, BMSC-loaded NCSilMA (organoids) achieved regeneration, up-regulated osteogenic genes enhanced cell colonization, collagen, polysaccharide deposition.

Language: Английский

Citations

0