High‐performance liquid chromatography analysis of alkaloids in various parts of lotus extracts with ion mobility spectrometry and mass spectrometry dual detection DOI
Hongbin Lu,

Ziqi Fang,

Binwang Yang

et al.

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 47(1)

Published: Dec. 14, 2023

Using high‐performance liquid chromatography coupled with electrospray ionization‐ion mobility spectrometry and mass spectrometry, we proposed a dual‐detection method for the identification profiling of alkaloids in various lotus parts including leaf, plumule, stem, seed epicarp, receptacle. The eluent from was split conducted to time‐of‐flight separately facilitate compound identification. In total, 23 kinds were identified based on m / z , drift time, retention alkaloid isomers such as lirinidine, N‐nornuciferine, O‐nornuciferine identical that are difficult differentiate using alone. this method, investigated changing dynamics accumulation leaves stems at different harvesting periods. total content showed an increasing trend growth development leave stem. Overall, developed dual detection has advantages high peak capacity sensitivity compared conventional facilitates detected compounds.

Language: Английский

Rapid analysis of the chemical constituents in Qiangli Dingxuan tablets using ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry DOI
Xin Gao, Yaxuan Wang, Wenjun Sun

et al.

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(2)

Published: Jan. 1, 2024

Qiangli Dingxuan (QLDX) tablet is a widely recognized traditional Chinese medicine formula that has been extensively used in China for decades to treat vertigo, tinnitus, and dizziness owing its outstanding therapeutic outcomes. However, the complexity of chemical components this makes it challenging separate identify these components. This study presented an effective sensitive strategy rapid separation simultaneous structural identification QLDX using ultra‐performance liquid chromatography with quadrupole time‐of‐flight tandem mass spectrometry UNIFI platform. Based on retention times, accurate masses, fragment ions, related literature, authentic standards, 119 compounds were identified or tentatively characterized; included 9 iridoids, 12 lignans, 21 phenylpropanoids, 27 flavonoids, 7 phthalides, 43 others. Among them, 36 confirmed reference standards. The representative various structures studied by analyzing their fragmentation patterns characteristic ions. In conclusion, established approach characterizing constituents tablet. proposed provides basis qualitative analysis quality control manufacturing process beneficial advancing investigations into efficacy mechanism action

Language: Английский

Citations

4
Comprehensive Lipid Metabolites-Pyroptosis Profiling Uncovers the Therapeutic Efficacy of Mailuo Shutong Pill Against Thromboangiitis Obliterans DOI

Chenhan He,

Peipei Zhou, Zhibo Zhang

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Shedding light on the composition and fading mechanisms of dye molecules in Phellodendron amurense Rupr. Dyed silk DOI
Xudong Zhou, Yujie Guo, Hui Zhang

et al.

Journal of Photochemistry and Photobiology A Chemistry, Journal Year: 2024, Volume and Issue: 451, P. 115496 - 115496

Published: Jan. 29, 2024

Language: Английский

Citations

2
Integrated microbiome and metabolomics analysis reveal the relationship between plant-specialized metabolites and microbial community in Phellodendron amurense DOI Creative Commons

Wanran Zhang,

Ranran Gao, Lixia Tian

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 21, 2024

Phellodendron amurense is the essential source of bisbenzylisoquinoline alkaloids (BIAs), making it a highly valued raw material in traditional Chinese medicine. The plant’s root secondary metabolism intricately linked to microbial communities that surround it. However, root-associated microbiomes P. , as well potential correlation between its bioactive compounds and these microbiomes, remain poorly understood. Here, metabolic profiles root, rhizosphere, bulk soils revealed dramatic differences relative content plant-specialized metabolites. A total 31, 21, 0 specialized metabolites were identified rhizosphere soil, respectively, with higher seven major BIAs observed compared soils. composition was noticeably distinct from endospheric microbiome. phylum Cyanobacteria accounted for over 60% endosphere communities, α-diversity lowest. Targeted BIAs, namely, berberine, palmatine, magnocurarine, phellodendrine, jatrorrhizine, tetrahydropalmatine, magnoflorine, significantly positively correlated Nectriaceae Sphingobacteriaceae. This study has illuminated intricate interaction networks microorganisms their key chemical compounds, providing theoretical foundation discovering biological fertilizers laying groundwork cultivating high-quality medicinal plants.

Language: Английский

Citations

2
Establishment of chemical fingerprint and determination of main active ingredients in Centellae Herba by HPLC combined with multivariate chemometrics analysis DOI
Duan Yu,

Guo Zhongjing,

Zhimin Zhao

et al.

Separation Science Plus, Journal Year: 2024, Volume and Issue: 7(6)

Published: May 10, 2024

Abstract In this paper, a simple and efficient high‐performance liquid chromatography method was established to analyze the chemical composition fingerprint of Centellae Herba (CHA) determine five triterpenoid active components in CHA, namely, asiaticoside B (AB), madecassoside, asiaticoside, madecasic acid, asiaticoic acid. The separation compounds carried out on an XD‐C18 column (250 mm × 4.6 mm, 5 µm), wavelength ultraviolet detector set 205 nm, mobile phase composed acetonitrile −0.05% phosphoric 2 mmol/L β‐cyclodextrin aqueous solution, gradient elution at flow rate 1.0 mL/min. A general chromatographic consisting 20 characteristic peaks 18 batches CHA samples established, were classified evaluated by similarity analysis, hierarchical cluster principal component analysis combined with multivariate econometric analysis. quantitative all calibration curves showed good linear regression test range ( r > 0.999), average recovery 96.12%–104.63%, relative standard deviations repeatability stability less than 2.00%. results show that is accurate effective can be used for comprehensive quality control CHA.

Language: Английский

Citations

1
Induction of Tetraploids in Phellodendron amurense Rupr. and Its Effects on Morphology and Alkaloid Content DOI Creative Commons
Jing Li, Ning Yu,

Can-Can Lv

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(9), P. 2090 - 2090

Published: Sept. 13, 2024

Phellodendron amurense Rupr. is a precious medicinal tree species in northeast China. However, P. resources have been severely destroyed due to uncontrolled overharvest and the limited innovation of new germplasms by traditional cross-breeding. In this study, polyploid breeding was introduced improvement program amurense. Fifty-four tetraploid plants were first produced colchicine-induced adventitious bud chromosome doubling stem segment explants. The induction frequency reached 36.16% (1.0 g L−1 colchicine solution for 48 h treatment) 50.00% (2.0 24 treatment), respectively, showing high efficiency somatic based on organogenesis system. Tetraploidization resulted significant phenotypic variation, such as larger thicker leaves, stems, bigger stomata. Ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) analysis identified 59 differentially accumulated alkaloids (DAAs) between leaf samples tetraploids, including 32 upregulated 27 downregulated stems. For both samples, 18 DAAs diploids with 16 leaves 8 suggesting that polyploidization caused alterations alkaloid contents stems main compounds, berberine, jatrorrhizine, phellodendrine, palmatine, increased significantly and/or after polyploidization. This finding implied might be an effective approach improving amurense, beneficial preserving exploiting natural resources.

Language: Английский

Citations

0
High‐performance liquid chromatography analysis of alkaloids in various parts of lotus extracts with ion mobility spectrometry and mass spectrometry dual detection DOI
Hongbin Lu,

Ziqi Fang,

Binwang Yang

et al.

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 47(1)

Published: Dec. 14, 2023

Using high‐performance liquid chromatography coupled with electrospray ionization‐ion mobility spectrometry and mass spectrometry, we proposed a dual‐detection method for the identification profiling of alkaloids in various lotus parts including leaf, plumule, stem, seed epicarp, receptacle. The eluent from was split conducted to time‐of‐flight separately facilitate compound identification. In total, 23 kinds were identified based on m / z , drift time, retention alkaloid isomers such as lirinidine, N‐nornuciferine, O‐nornuciferine identical that are difficult differentiate using alone. this method, investigated changing dynamics accumulation leaves stems at different harvesting periods. total content showed an increasing trend growth development leave stem. Overall, developed dual detection has advantages high peak capacity sensitivity compared conventional facilitates detected compounds.

Language: Английский

Citations

1