IEEE Photonics Technology Letters,
Journal Year:
2024,
Volume and Issue:
36(14), P. 881 - 884
Published: May 27, 2024
Reflectance
confocal
microscopy
is
widely
used
in
several
applications
that
require
non-destructive
optical
three-dimensional
(3D)
imaging.
However,
the
acquisition
speed
of
conventional
limited
by
mechanical
3D
scanning.
Furthermore,
acquiring
data
from
a
sample
with
excessively
different
degrees
reflectance
difficult.
We
propose
system
combines
high
dynamic
range
(HDR)
technique
and
parallelized
detection
to
achieve
high-speed
volumetric
imaging
for
samples
varying
reflectivity.
Our
sequentially
acquires
two-dimensional
color
images
projecting
patterns
onto
objects
using
focus
tunable
lens
axially
scanning,
thereby
creating
profile.
By
modulating
power
light
source
synchronization
axial
scanning
direction,
two
profiles
are
produced
fused
them
reconstruct
an
HDR
demonstrate
proposed
can
capture
thorough
even
wide
Biomedical Optics Express,
Journal Year:
2025,
Volume and Issue:
16(2), P. 837 - 837
Published: Jan. 15, 2025
Optical
diffraction
tomography
(ODT)
is
an
important
technique
for
three-dimensional
(3D)
imaging
of
semi-transparent
biological
samples,
enabling
volumetric
visualization
living
cells,
cultures,
and
tissues
without
the
need
exogenous
dyes.
However,
ODT
faces
significant
challenges
in
complex
specimens
due
to
limited
specificity
refractive
index
(RI)
coupled
relationship
between
absorption
phase
image
formation.
Here,
we
present
multi-modal
transport
intensity
(MM-TIDT),
a
high-speed
3D
microscopy
that
integrates
electrically
tunable
lens
with
modified
illumination
patterns
decouple
information.
Leveraging
dual
schemes-circular
annular
apertures-MM-TIDT
acquires
two
stacks,
facilitating
accurate
decoupling.
Based
on
alternating
direction
method
multipliers
(ADMM)
framework
total
variation
(TV)
non-negativity
regularization,
our
reconstructs
distributions
fluorescence
RI
high
accuracy
robustness.
Experimental
validation
fluorescent
polystyrene
microspheres,
Spirulina
specimens,
DAPI-labeled
C166
cells
demonstrates
capability
MM-TIDT
resolve
fine
structural
details
across
diverse
sample
types,
providing
versatile
platform
exploring
dynamic
processes
intricate
cellular
interactions.
Abstract
Intensity
diffraction
tomography
(IDT)
and
fluorescence
dual‐modality
imaging
facilitate
a
comprehensive
analysis
of
biological
components
their
interactions.
However,
long‐term
is
typically
compromised
by
environmental
thermal
fluctuations
mechanical
disturbances
from
the
microscope,
leading
to
time‐varying
aberrations
focus
drift
that
degrades
performance
accuracy
colocalization.
To
counter
these
issues,
an
adaptive
optics‐assisted
3D
method
(AO‐FIDT)
developed.
The
utilizes
innovative
iterative
ptychographic
approach,
paired
with
annular
matched
illumination
conditions,
precisely
reconstruct
IDT
results
characterize
in
real‐time.
Furthermore,
feedback
on
real‐time
point
spread
function
system
provided
synchronously
correct
results.
efficacy
precision
AO‐FIDT
are
confirmed
through
long‐term,
high‐resolution
HeLa
cells.
scrutinizing
morphological
characteristics
subcellular
organelles
live
COS‐7
cells,
including
progressive
sphericity
mitochondria
under
phototoxicity,
monitoring
continuous
changes
mitochondrial
dynamics
throughout
cell
division
process,
broad
applicability
analyzing
organelle
structure
demonstrated.
Abstract
Liquid
lens
offers
a
novel
approach
to
achieving
large
depth
of
field,
wide
viewing
angle,
high
speed,
and
high-quality
imaging
in
zoom
optical
systems.
However,
the
aperture
reliability
limit
lens’s
performance
various
applications.
The
liquid
material
is
crucial
for
large-aperture
lens.
To
solve
dielectric
failure
problem
associated
with
aperture,
we
first
reveal
mechanism
based
on
transport
properties
electrolyte
solutions
impact
electrochemical
reaction
rates
from
physical
chemistry
so
as
propose
theoretical
method
suppress
fundamentally.
Based
this
theory,
develop
series
non-aqueous
organic
high-voltage
failure.
Next,
identify
optimal
formulation
comprehensive
fabricate
centimeter-level
electrowetting
This
features
an
power
variation
range
−11.98
m
−1
12.93
,
clear
function,
which
can
enlarge
field
view
adjustment
holographic
reconstructions
while
maintaining
excellent
edge
clarity
reconstructed
images.
proposed
effectively
suppresses
under
voltage,
demonstrates
performance,
holds
exciting
potential
applications
3D
display,
precision
measurement,
biomedical
observation,
more.
Abstract
A
novel
high‐speed,
high‐resolution
3D
microscopy
technique
named
BP‐TIDT
is
presented
that
quantifies
the
refractive
index
(RI)
distribution
of
label‐free,
transparent
samples.
This
method
combines
a
bi‐plane
detection
scheme
(BP)
with
transport
intensity
diffraction
tomography
(TIDT),
effectively
circumventing
need
for
matched
illumination
conditions
under
high
numerical
aperture
(NA)
objectives,
which
enables
15
fps
volume
rates
and
326
nm
lateral
resolution.
The
effectiveness
accuracy
proposed
approach
are
validated
through
imaging
polystyrene
microspheres
HepG2
cells.
Moreover,
wide‐ranging
applicability
demonstrated
by
investigating
subcellular
organelle
motion,
including
mitochondria
lipid
droplets,
as
well
macroscopic
apoptosis
process
in
living
COS‐7
To
best
current
knowledge,
this
first
time
spatial‐temporal
resolution
dynamic
ODT
results
obtained
non‐interferometric
motion‐free
manner,
highlighting
potential
advancing
research
on
cellular
processes.
Optics Letters,
Journal Year:
2025,
Volume and Issue:
50(5), P. 1457 - 1457
Published: Jan. 23, 2025
Non-interferometric
synthetic
aperture
imaging
(SAI)
shows
significant
potential
in
Earth
observation,
astronomy,
and
remote
sensing.
However,
these
methods
often
involve
time-consuming
processes
for
wave
field
acquisition
iterative
image
reconstruction.
In
this
Letter,
we
present
a
non-iterative
far-field
method,
macroscopic
space-domain
Kramers-Kronig
relations
(MSKR-SAI).
Unlike
traditional
Fourier
ptychography
(FP),
MSKR-SAI
bypasses
redundant
iterations
requirements
highly
overlapping
images,
reducing
reconstruction
time
from
4.97
s
to
0.17
s-a
26-fold
speedup.
By
utilizing
only
six
sub-aperture
intensity
reconstructs
complex
amplitude
information
synthesizes
the
fully
determinist
manner.
Simulations
experimental
results
show
twofold
resolution
improvement
with
accurate
detail
recovery
minimal
artifacts.
Furthermore,
maintains
robustness
even
when
are
not
strictly
met.
The
combination
of
reconstruction,
noise
resilience,
computational
efficiency
positions
as
promising
method
high-resolution,
artifact-free
imaging.
Abstract
Fluorescence
microscopy
is
an
indispensable
tool
for
investigating
cellular
and
tissue‐level
biology,
yet
its
performance
often
limited
by
optical
diffraction,
aberrations,
noise,
resulting
in
suboptimal
imaging
quality.
Traditional
adaptive
optics
(AO)
methods
typically
rely
on
additional
hardware,
such
as
wavefront
sensors,
to
measure
correct
system
which
can
be
both
complex
costly.
Here,
a
computational
technique
based
sparse
blind
deconvolution
(CAO‐SBD)
introduced,
uses
single
blurred
image
estimate
aberrations
perform
deblurring.
By
incorporating
priors
of
fluorescent
specimens
with
Zernike
polynomial‐based
aberration
modeling,
CAO‐SBD
allows
the
simultaneous
reconstruction
aberrated
point
spread
function
(PSF)
sample
information,
eliminating
need
precise
PSF
calibration.
This
method
outperforms
traditional
Richardson‐Lucy
enhancing
robustness
noise
stabilizing
process
through
correction.
Experimental
results
bovine
pulmonary
artery
endothelial
cells
demonstrate
that
significantly
enhances
resolution
contrast
across
wide‐field
confocal
fluorescence
microscopic
systems,
positioning
powerful
high‐resolution
biological
broad
applications.
