Injectable smart stimuli-responsive hydrogels: pioneering advancements in biomedical applications

Biomaterials Science, Journal Year: 2023, Volume and Issue: 12(1), P. 8 - 56

Published: Oct. 25, 2023

Hydrogels have established their significance as prominent biomaterials within the realm of biomedical research. However, injectable hydrogels garnered greater attention compared with conventional counterparts due to excellent minimally invasive nature and adaptive behavior post-injection. With rapid advancement emerging chemistry deepened understanding biological processes, contemporary been endowed an "intelligent" capacity respond various endogenous/exogenous stimuli (such temperature, pH, light magnetic field). This innovation has spearheaded revolutionary transformations across fields such tissue engineering repair, controlled drug delivery, disease-responsive therapies, beyond. In this review, we comprehensively expound upon raw materials (including natural synthetic materials) principles these advanced hydrogels, concurrently providing a detailed discussion prevalent strategies for conferring stimulus responsiveness. Finally, elucidate latest applications "smart" stimuli-responsive in domain, offering insights into prospects.

Language: Английский

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Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration

Gels, Journal Year: 2025, Volume and Issue: 11(1), P. 42 - 42

Published: Jan. 7, 2025

Articular cartilage faces challenges in self-repair due to the lack of blood vessels and limited chondrocyte concentration. Polydeoxyribonucleotide (PDRN) shows promise for promoting growth regeneration, but its delivery has been injections. Continuous PDRN is crucial effective regeneration. This study explores using gelatin methacrylate (gelMA) hydrogel, crosslinked with visible light riboflavin 5′-phosphate sodium (RF) as a photoinitiator, sustained release. GelMA hydrogel’s synthesis was confirmed through spectrophotometric techniques, demonstrating successful group incorporation. PDRN-loaded gelMA hydrogels displayed varying pore sizes, swelling ratios, degradation rates, mechanical properties based on They showed release biocompatibility, 14% gelMA-PDRN composition performing best. Glycosaminoglycan (GAG) activity higher hydrogels, indicating positive effect formation. RT-PCR analysis revealed increased expression cartilage-specific genes (COL2, SOX9, AGG) gelMA-PDRN. Histological assessments rabbit defect model demonstrated superior regenerative effects hydrogels. highlights potential tissue engineering, providing promising approach

Language: Английский

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Cryopreservation of Cell Sheets for Regenerative Therapy: Application of Vitrified Hydrogel Membranes

Gels, Journal Year: 2023, Volume and Issue: 9(4), P. 321 - 321

Published: April 10, 2023

Organ transplantation is the first and most effective treatment for missing or damaged tissues organs. However, there a need to establish an alternative method organ due shortage of donors viral infections. Rheinwald Green et al. established epidermal cell culture technology successfully transplanted human-cultured skin into severely diseased patients. Eventually, artificial sheets cultured were created, targeting various organs, including epithelial sheets, chondrocyte myoblast sheets. These have been used clinical applications. Extracellular matrix hydrogels (collagen, elastin, fibronectin, laminin), thermoresponsive polymers, vitrified hydrogel membranes as scaffold materials prepare Collagen major structural component basement tissue proteins. (collagen vitrigel), created from collagen through vitrification process, are composed high-density fibers expected be carriers transplantation. In this review, essential technologies sheet implantation described, membranes, their cryopreservation applications in regenerative medicine.

Language: Английский

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Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells

Polymers, Journal Year: 2023, Volume and Issue: 15(15), P. 3183 - 3183

Published: July 27, 2023

To guide therapeutic strategies and to monitor the state changes in disease, a low-cost, portable, easily fabricated microfluidic-chip-integrated three-dimensional (3D) microchamber was designed for capturing analyzing breast cancer cells. Optimally, colorimetric sensor array integrated into microfluidic chip discriminate metabolites of The ultraviolet polymerization characteristic poly(ethylene glycol) diacrylate (PEGDA) hydrogel utilized rapidly fabricate three-layer with structure under noninvasive 365 nm laser irradiation. 2-Hydroxyethyl methacrylate (HEMA) added prepolymer order increase adhesive capacity microchip’s surface 1-Vinyl-2-pyrrolidone (NVP) improve toughness reduce swelling composite. A non-toxic 3D microarray (60 mm × 20 3 mm) low immunogenicity high hydrophilicity created simulate real physiological microenvironment tissue. crisscross channels were ensure homogeneous seeding density. This material displayed excellent biocompatibility tunable physical properties compared traditional materials can be directly processed obtain most desirable microstructure. feasibility using PEGDA real-time online detection cells’ metabolism confirmed specifically 16 kinds porphyrin, porphyrin derivatives, indicator dyes. results principal component analysis (PCA), hierarchical cluster (HCA), linear discriminant (LDA) suggest that metabolic liquids different cells distinguished developed chip. has potential practicable applicability distinguishing normal cancerous

Language: Английский

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Influence of the Structural Mechanics and Surface Properties of Injectable Chitosan Methacrylate-Based Hydrogels for Autologous Chondrocyte Implantation

JOM, Journal Year: 2024, Volume and Issue: 76(10), P. 5713 - 5734

Published: Aug. 12, 2024

Language: Английский

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