A facile strategy to fabricate stretchable, low hysteresis and adhesive zwitterionic elastomers by concentration-induced polymerization for wound healing

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 153804 - 153804

Published: July 6, 2024

Language: Английский

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Amyloid β-Peptide Segment Conjugated Side-Chain Proline-Based Polymers as Potent Inhibitors in Lysozyme Amyloidosis

Bioconjugate Chemistry, Journal Year: 2024, Volume and Issue: 35(3), P. 312 - 323

Published: Feb. 29, 2024

Developing effective amyloidosis inhibitors poses a significant challenge due to the dynamic nature of protein structures, complex interplay interfaces in protein–protein interactions, and irreversible amyloid assembly. The interactions amyloidogenic polypeptides with other peptides play pivotal role modulating fibril formation. This study presents novel approach for designing synthesizing interaction surfaces using segments derived from amyloid-promoting sequence β-peptide [VF(Aβ(18–19)/FF(Aβ(19–20)/LVF(Aβ(17–19)/LVFF(Aβ(17–20)], where VF, FF, LVF LVFF stands valine phenylalanine dipeptide, leucine tripeptide tetrapeptide, respectively. These are conjugated side-chain proline-based methacrylate polymers serving as potent lysozyme demonstrating reduced cytotoxicity aggregations. Di-, tri-, tetra-peptide chain transfer agents (CTAs) were synthesized used reversible addition–fragmentation polymerization tert-butoxycarbonyl (Boc)-proline methacryloyloxyethyl ester (Boc-Pro-HEMA). Deprotection Boc-groups proline pendants resulted water-soluble defined peptide ends peptide–polymer bioconjugates. Among them, LVFF-conjugated polymer acted inhibitor significantly suppressed amyloidosis, finding supported by comprehensive spectroscopic, microscopic, computational analyses. results unveil synergistic effect between segment-derived polymers, offering new prospects targeting amyloidosis.

Language: Английский

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Exploring the amyloid degradation potential of nanoformulated carrageenan-bridging in vitro and in vivo perspectives

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 134814 - 134814

Published: Aug. 20, 2024

Language: Английский

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Amino Acid-Based Polymer-Coated Silver Nanoparticles as Insulin Fibril Inhibitors

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(10), P. 8705 - 8716

Published: May 16, 2023

To explore the impact of polymer-coated silver nanoparticles (PC-AgNPs) on extent insulin aggregation process, herein, we have synthesized three copolymers comprising poly(ethylene glycol) methyl ether methacrylate (PEGMA) and tert-butoxycarbonyl (Boc)-protected amino acid (alanine, leucine, phenylalanine) containing monomers, via reversible addition-fragmentation chain transfer (RAFT) polymerization. After deprotection Boc groups, as-prepared water-soluble were coated (Ag NPs), role these NPs pathways was examined by multifarious spectroscopic microscopic techniques. The inhibitory effect against fibrillation process found to be related surface properties NPs, with highest detected for phenylalanine-based Ag (PPhe-AgNPs). Using circular dichroism (CD) spectroscopy Nile red (NR) fluorescence spectroscopy, investigated conformational changes hydrophobic interaction in inhibiting upon treatment PC-AgNPs. Furthermore, PC-AgNPs also able disintegrate matured fibrils efficiently decreased fibril-induced cytotoxicity, as confirmed transmission electron microscopy (TEM) hemolysis study, respectively. Together, our findings established novel acid-based potent nanomaterials 77–96% fibril inhibition marked disaggregation fibrils.

Language: Английский

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A chemically engineered water-soluble block copolymer for redox responsive SO₂ release in antibacterial therapy

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(34), P. 8454 - 8464

Published: Jan. 1, 2024

A redox-responsive sulfur dioxide (SO 2 )-releasing water-soluble amphiphilic block copolymer was synthesized, and it exhibited sustainable SO release antibacterial activity against Gram-positive bacteria Bacillus subtilis .

Language: Английский

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Cost-effective and natural-inspired lotus root/GelMA scaffolds enhanced wound healing via ROS scavenging, angiogenesis and reepithelialization

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134496 - 134496

Published: Aug. 10, 2024

Language: Английский

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Hydrogel microspheres encapsulating lysozyme/MXene for photothermally enhanced antibacterial activity and infected wound healing

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135527 - 135527

Published: Sept. 10, 2024

Language: Английский

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Harnessing the power of ginger leaf polysaccharide: A potential strategy to combat Aβ-induced toxicity through the Wnt/β-catenin pathway

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 303, P. 140692 - 140692

Published: Feb. 4, 2025

Language: Английский

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Fenugreek seeds as a natural source of L-arginine-encapsulated lipid nanoparticles against diabetes

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 27, 2025

Diabetes, affecting over 10.5% of the global population, leads to severe health complications and economic burdens, highlighting urgent need for effective therapeutic approaches. Current treatments are often insufficient, prompting exploration novel agents delivery mechanisms. This study addresses this gap by investigating roles L-arginine (identified as a target drug candidate through network pharmacology) in diabetes management, while also evaluating lipid nanocarriers synthesized from fenugreek seed oil improved delivery. Our docking analyses revealed L-arginine's strong interactions with diabetes-target genes (CYP1A2, CYP2C19, NFKB), multiple hydrogen bonds binding energies ranging − 7.2 8.9 kcal/mol. Encapsulated nanoparticles were characterized using UV-Visible spectroscopy, showing absorbance peaks at 415 nm simple 521 L-arginine-loaded nanoparticles. Scanning electron microscopy confirmed an average nanoparticle size 100.2 nm, zeta potential analysis indicated neutral surface charge (− 9.37 mV). Antioxidative activity showed 84.44% inhibition IC50 value 40.5 µg/mL The inhibited albumin denaturation 81.10% alpha-amylase 89.30%, surpassing metformin (78.43% 1000 µg/mL). Hemolysis percentage was minimal 10.54%. These findings demonstrate anti-diabetic agent highlight efficacy innovative systems, providing foundation advancing interventions against diabetes.

Language: Английский

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Inhibition of Insulin Amyloid Fibrillogenesis Using Antioxidant Copolymers with Dopamine Pendants

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Abstract Amyloid aggregation, intricately related to various neurodegenerative and metabolic diseases, presents a significant growing health challenge. Dopamine, potent antioxidant, plays pivotal role in modulating protein misfolding by leveraging its anti‐amyloidogenic neuroprotective properties. However, biological applications are limited poor aqueous solubility suboptimal biocompatibility. To address these challenges, water‐soluble copolymers ( DP1 ‐ DP3 ) featuring dopamine glucose side‐chain pendants fabricated investigated for their efficacy inhibiting amyloid fibril formation from insulin beta (A β 42 peptide. The effects of on fibrillation assessed using several biophysical techniques, which demonstrate excellent radical scavenging properties the remarkable copolymer suppressing fibrillation, achieving ≈97% inhibition. Isothermal titration calorimetry (ITC) fluorescence binding experiments carried out quantify insulin‐ complex formation. Molecular dynamics simulations validate ability prevent fibrillogenesis both A . These studies beneficial interactions between amyloidogenic protein/peptide, facilitating stability resulting complexes. Overall, present findings suggest that dopamine‐based antioxidant polymers hold potential as advanced therapeutic agents preventing disorders.

Language: Английский

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