Biomedical Materials,
Journal Year:
2024,
Volume and Issue:
20(1), P. 015009 - 015009
Published: Nov. 25, 2024
Abstract
Thiol-norbornene
photoclick
hydrogels
are
highly
efficient
in
tissue
engineering
applications
due
to
their
fast
gelation,
cytocompatibility,
and
tunability.
In
this
work,
we
utilized
the
advantageous
features
of
polyethylene
glycol
(PEG)-thiol-ene
resins
enable
fabrication
complex
heterogeneous
scaffolds
using
3D
bioprinting
in-air
drop
encapsulation
techniques.
We
demonstrated
that
photoclickable
PEG-thiol-ene
could
be
tuned
by
varying
ratio
PEG-dithiol
PEG
norbornene
generate
a
wide
range
mechanical
stiffness
(0.5–12
kPa)
swelling
ratios.
Importantly,
all
formulations
maintained
constant,
rapid
gelation
time
(<0.5
s).
used
resin
biological
projection
microstereolithography
(BioP
µ
SL)
print
structures
with
geometric
fidelity
biocompatibility
printing
cell-laden
microgrids.
Moreover,
gelling
kinetics
permitted
high-throughput
tunable,
microgels
air
apparatus
(BioIDEA).
these
support
cell
viability
assembled
into
gradient
structure.
This
resin,
along
BioP
SL
BioIDEA
technology,
will
allow
tissues
mimic
native
cellular
gradients.
The
engineered
controlled
microscale
porosity
including
engineering,
biosensing,
vitro
models.
Advanced Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 5, 2025
Engineered
living
systems
(ELSs)
represent
purpose-driven
assemblies
of
components,
encompassing
cells,
biomaterials,
and
active
agents,
intricately
designed
to
fulfill
diverse
biomedical
applications.
Gelatin
its
derivatives
have
been
used
extensively
in
ELSs
owing
their
mature
translational
pathways,
favorable
biological
properties,
adjustable
physicochemical
characteristics.
This
review
explores
the
intersection
gelatin
with
fabrication
techniques,
offering
a
comprehensive
examination
synergistic
potential
creating
for
various
applications
biomedicine.
It
offers
deep
dive
into
gelatin,
including
structures
production,
sources,
processing,
properties.
Additionally,
techniques
employing
derivatives,
generic
microfluidics,
3D
printing
methods.
Furthermore,
it
discusses
based
on
regenerative
engineering
as
well
cell
therapies,
bioadhesives,
biorobots,
biosensors.
Future
directions
challenges
are
also
examined,
highlighting
emerging
trends
areas
improvements
innovations.
In
summary,
this
underscores
significance
gelatin-based
advancing
lays
groundwork
guiding
future
research
developments
within
field.
Macromolecular Rapid Communications,
Journal Year:
2024,
Volume and Issue:
45(7)
Published: Jan. 25, 2024
Photocuring
3D
printing
of
hydrogels,
with
sophisticated,
delicate
structures
and
biocompatibility,
attracts
significant
attention
by
researchers
possesses
promising
application
in
the
fields
tissue
engineering
flexible
devices.
After
years
development,
photocuring
technologies
hydrogel
inks
make
great
progress.
Herein,
techniques
including
direct
ink
writing
(DIW),
stereolithography
(SLA),
digital
light
processing
(DLP),
continuous
liquid
interface
production
(CLIP),
volumetric
additive
manufacturing
(VAM),
two
photon
polymerization
(TPP)
are
reviewed.
Further,
raw
materials
for
(photocurable
polymers,
monomers,
photoinitiators,
additives)
applications
devices
also
At
last,
current
challenges
future
perspectives
hydrogels
discussed.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(36)
Published: April 18, 2024
Abstract
Decellularized
small
intestine
submucosa
(dSIS)
is
a
promising
biomaterial
for
promoting
tissue
regeneration.
Isolated
from
the
submucosal
layer
of
animal
jejunum,
SIS
rich
in
extracellular
matrix
(ECM)
proteins,
including
collagen,
laminin,
and
fibronectin.
Following
mild
decellularization,
dSIS
becomes
an
acellular
that
supports
cell
adhesion,
proliferation,
differentiation.
Conventional
usually
obtained
by
thermal
crosslinking,
which
yields
soft
scaffold
with
low
stability.
To
address
these
challenges,
modified
methacrylate
groups
photocrosslinking
into
stable
hydrogels.
However,
has
not
been
clickable
handles
orthogonal
crosslinking.
Here,
development
norbornene‐modified
dSIS,
named
dSIS‐NB,
via
reacting
amine
carbic
anhydride
acidic
aqueous
reaction
conditions
reported.
Using
triethylamine
(TEA)
as
base
catalyst,
high
degrees
NB
substitution
on
are
obtained.
In
addition
to
describing
synthesis
its
adaptability
hydrogel
crosslinking
cancer
vascular
engineering
explored.
Impressively,
compared
physically
crosslinked
collagen
matrices,
orthogonally
dSIS‐NB
hydrogels
supported
rapid
dissemination
cells
superior
vasculogenic
angiogenic
properties.
also
exploited
versatile
bioink
3D
bioprinting.
Lab on a Chip,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Tissue
engineering
offers
immense
potential
for
addressing
the
unmet
needs
in
repairing
tissue
damage
and
organ
failure
through
enhanced
vascularization
using
microfluidic
bioprinting
methods.
ACS Applied Bio Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 3, 2025
Driven
by
the
increasing
need
for
biofabrication
of
complex
hydrogels,
this
work
introduces
a
class
fish-porcine
composite
hydrogels
that
combine
rapid,
tunable
photo-cross-linking
with
microparticle
reinforcement
advanced
3D
printing.
Here,
precross-linked
porcine
gelatin
(methacrylated
gelatin,
MPG)
microparticles
are
incorporated
into
methacrylated
fish
(MFG)
matrix
to
produce
robust
yet
easily
processable
hydrogels.
Nuclear
magnetic
resonance
(NMR)
confirmed
degree
methacrylation,
while
scanning
electron
microscopy
(SEM)
revealed
hierarchical
porosity
vital
tissue
integration.
Detailed
Mastersizer
measurements
characterized
size
distributions
MPG
microparticles,
and
rheological
tests
demonstrated
hydrogels'
strong
shear-thinning
behavior,
an
essential
trait
extrusion-based
embedded
Thermal
(TGA,
DSC)
mechanical
(compression)
analyses
show
microparticle-reinforced
achieve
improved
thermal
stability,
adjustable
mass
swelling
ratio,
customizable
compressive
moduli.
As
proof
concept,
these
composites
validated
in
digital
light
processing
(DLP)
printing
microfluidic
constructs
as
support
bath
geometries.
This
platform
provides
unique
synergy
easy
UV
cross-linkability,
features,
versatility.
advancement
underscores
potential
materials
foundational
engineering,
opening
new
avenues
creating
complex,
biocompatible
structures
properties.
Macromolecular Materials and Engineering,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 5, 2025
Abstract
Using
additive
manufacturing
(AM)
technologies
for
the
fabrication
of
advanced
polymeric
objects
is
a
logical
progression
to
realize
their
potential
within
engineering
applications
that
demand
complex
geometries.
Thiol‐X
chemistry
has
presented
itself
as
diverse
and
invaluable
toolkit
accomplish
such,
satisfying
both
processing
requirements
properties
desired
fabricate
range
using
variety
AM
technologies.
The
“click”
nature
many
thiol‐X
reactions,
mechanisms
polymerization,
functional
group
tolerance
orthogonality,
desirable
achievable
with
presents
synergistic
opportunity
few
other
chemistries
can
broadly
facilitate.
As
result,
use
gained
rapid
traction
in
recent
years
it
now
case
are
being
viewed
two
sides
same
coin,
whereby
scope
limitations
whole
system
appreciated.
This
review
highlights
advancements,
status,
scope,
opportunities
achieve
polymers
by
critically
examining
structure‐property‐processing‐performance
relationships
between
different
various
Biomaterials Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
The
development
of
bioink-based
3D-printed
scaffolds
has
revolutionized
bone
tissue
engineering
(BTE)
by
enabling
patient-specific
and
biomimetic
constructs
for
regeneration.
