Probiotics fortify intestinal barrier function: a systematic review and meta-analysis of randomized trials

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 24, 2023

Background Probiotics play a vital role in treating immune and inflammatory diseases by improving intestinal barrier function; however, comprehensive evaluation is missing. The present study aimed to explore the impact of probiotics on related function, inflammation, microbiota composition. A systematic review meta-analyses were conducted. Methods Four major databases (PubMed, Science Citation Index Expanded, CENTRAL, Embase) thoroughly searched. Weighted mean differences calculated for continuous outcomes with corresponding 95% confidence intervals (CIs), heterogeneity among studies was evaluated utilizing I2 statistic (Chi-Square test), data pooled using random effects meta-analyses. Results Meta-analysis from total 26 RCTs (n = 1891) indicated that significantly improved gut function measured levels TER (MD, 5.27, CI, 3.82 6.72, P < 0.00001), serum zonulin (SMD, -1.58, -2.49 -0.66, 0.0007), endotoxin -3.20, -5.41 -0.98, 0.005), LPS -0.47, -0.85 -0.09, 0.02). Furthermore, probiotic groups demonstrated better efficacy over control reducing factors, including CRP, TNF-α, IL-6. can also modulate structure boosting enrichment Bifidobacterium Lactobacillus. Conclusion work revealed could improve alleviate inflammation microbial dysbiosis. Further high-quality are warranted achieve more definitive conclusion. Clinical trial registration https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=281822 , identifier CRD42021281822.

Language: Английский

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The role of Lactobacillus in inflammatory bowel disease: from actualities to prospects

Cell Death Discovery, Journal Year: 2023, Volume and Issue: 9(1)

Published: Sept. 29, 2023

Abstract Inflammatory Bowel Disease (IBD), a chronic nonspecific intestinal inflammatory disease, is comprised of Ulcerative Colitis (UC) and Crohn’s (CD). IBD closely related to systemic reaction affects the progression many extraintestinal diseases. As one representative bacteria for probiotic-assisted therapy in IBD, multiple strains Lactobacillus have been proven alleviate damage strengthen immunological barrier, epithelial cell mucus barrier. also spares no effort alleviation IBD-related diseases such as Colitis-associated Colorectal cancer (CAC), Alzheimer’s (AD), Depression, Anxiety, Autoimmune Hepatitis (AIH), so on via gut-brain axis gut-liver axis. This article aims discuss role diseases, including its underlying mechanisms curative strategies from present future.

Language: Английский

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Mixed probiotics modulated gut microbiota to improve spermatogenesis in bisphenol A-exposed male mice

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 270, P. 115922 - 115922

Published: Jan. 1, 2024

Bisphenol A (BPA), an environmental endocrine disruptor (EDC), has been implicated in impairing intestinal and male reproductive dysfunction. The efficacy of gut microbiota modulation for BPA-exposed testicular dysfunction yet to be verified through research. Therefore, this study explored the potential mixed probiotics restoring spermatogenesis damage gut-testis axis under BPA exposure. We selected two strains (Lactobacillus rhamnosus Lactobacillus plantarum) with removal properties vitro mice model was established. mixture effectively reduced residue gut, serum, testis mice. Through 16 S rDNA-seq metabolomics sequencing, we uncovered that vitamin D metabolism bile acid levels abolished This perturbation linked increased abundance Faecalibaculum decreased Lachnospiraceae_NK4A136_group Ligilactobacillus. restored balance, enhancing barrier function reducing oxidative stress. improvement accompanied by a balance short-chain fatty acids (SCFAs). Remarkably, ameliorated repairing structures seminiferous tubules reversing arrested spermiogenesis. Further, enhanced testosterone-driven increases spermatogonial stem cells all stages sperm cells. Testicular transcriptome profiling these improvements degradation peroxisome pathways. These findings suggest significant interplay between microbiota, demonstrating probiotic intake could viable strategy combating subfertility issues caused

Language: Английский

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Ameliorative effect of an acidic polysaccharide from Phellinus linteus on ulcerative colitis in a DSS-induced mouse model

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130959 - 130959

Published: March 17, 2024

Language: Английский

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Dialdehyde starch-epicatechin gallate conjugate alleviates inflammation in lipopolysaccharide-stimulated RAW264.7 cells and dextran sulfate sodium-induced colitis mice

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141343 - 141343

Published: Feb. 21, 2025

Language: Английский

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Indole-3-Lactic Acid, a Tryptophan Metabolite of Lactiplantibacillus plantarum DPUL-S164, Improved Intestinal Barrier Damage by Activating AhR and Nrf2 Signaling Pathways

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(48), P. 18792 - 18801

Published: Nov. 23, 2023

A growing body of evidence suggests that microbial tryptophan metabolites play a crucial role in maintaining intestinal barrier stability and modulating host immunity. Our previous study showed the Lactiplantibacillus plantarum (L. ) DPUL-S164 intervention mice with high (Trp) diet promotes indole-3-lactic acid (ILA) production mice's tract ameliorates dextran sodium sulfate(DSS)-induced damage mice. In this study, we used HT-29 cell monolayer model to evaluate effect L. Trp (DPUL-S164-TM) on barrier. We found DPUL-S164-TM alleviated lipopolysaccharide (LPS)-induced inflammation by promoting expression tight junction proteins (ZO-1, occludin, claudin1), activating AhR Nrf2 signaling pathways, inhibiting NF-κB pathway. promotion protein activation pathway were dependent cells. Additionally, dramatic increase ILA content. Therefore, inferred plays key improving function alleviating inflammation.

Language: Английский

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Fecal microbiota transplantation alleviates experimental colitis through the Toll-like receptor 4 signaling pathway

World Journal of Gastroenterology, Journal Year: 2023, Volume and Issue: 29(30), P. 4657 - 4670

Published: Aug. 9, 2023

Fecal microbiota transplantation (FMT) has shown promising therapeutic effects on mice with experimental colitis and patients ulcerative (UC). FMT modulates the Toll-like receptor 4 (TLR4) signaling pathway to treat some other diseases. However, it remains unknown whether this modulation is also involved in treatment of UC.To clarify necessity TLR4 dextran sodium sulphate (DSS)-induced explain mechanism UC, through association analysis gut colon transcriptome mice.A mouse model was constructed wild-type (WT) TLR4-knockout (KO) mice. transplanted by gavage. Colon inflammation severity measured disease activity index (DAI) scoring hematoxylin eosin staining. Gut structure analyzed 16S ribosomal RNA sequencing. Gene expression obtained sequencing.The KO (DSS + Water) FMT) groups displayed indistinguishable body weight loss, length, DAI score, histology which showed that could not inhibit In treated FMT, relative abundance Akkermansia decreased, Lactobacillus became dominant. particular, compared those WT mice, scores were clearly decreased KO-DSS group. Microbiota a significant difference between dominant genus healthy The ineffectiveness related Akkermansia. Ontology enrichment differentially expressed genes each group mainly cytoplasmic translation cellular response DNA damage stimulus. top nine correlating included Aqp4, Clca4a, Dpm3, Fau, Mcrip1, Meis3, Nupr1 L, Pank3, Rps13 (|R| > 0.9, P < 0.01).FMT may ameliorate DSS-induced regulating pathway. composition maintain stability intestinal environment. bear great potential for colitis.

Language: Английский

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Quinoa protein and its hydrolysate ameliorated DSS-induced colitis in mice by modulating intestinal microbiota and inhibiting inflammatory response

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127588 - 127588

Published: Oct. 22, 2023

Language: Английский

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The prebiotics 2′-fucosyllactose prevent high-fat diet induced obesity via the promotion of thermogenesis and modulation of gut microbiota

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 119, P. 106287 - 106287

Published: June 15, 2024

Language: Английский

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FMT rescues mice from DSS-induced colitis in a STING-dependent manner

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Sept. 26, 2024

Fecal microbiota transplantation (FMT) is currently a promising therapy for inflammatory bowel disease (IBD). However, clinical studies have shown that there an obvious individual difference in the efficacy of FMT. Therefore, it pressing issue to identify factors influence FMT and find ways screen most suitable patients this therapy. In work, we targeted stimulator interferon genes (STING), DNA-sensing protein regulates host-defense. By comparing differential mice with different expression level STING, revealed provides treatment DSS-induced colitis STING-dependent manner. Mechanistically, exerts regulatory effect on differentiation intestinal Th17 cells macrophages, splenic Th1 Th2 cells, as well mesenteric lymph nodes via down-regulating colonic M1/M2 Th1/Th2 cell ratios, thereby improving imbalanced immune homeostasis inflamed intestine. Meanwhile, based 16SrDNA sequencing fecal samples, STING was found facilitate donor strain colonization recipients' gut, mainly

Language: Английский

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