Development of marker-based quantification methods fo r Diospyros Montana Roxb using DoE approach and in-silico anti-diabetic screening of selected phytoconstituents of the Diospyros genus DOI

Ashwini M. Kambalyal,

Parixit Bhandurge,

Nikhil Gawas

et al.

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: Dec. 26, 2024

Diabetes mellitus is a rising global health issue, necessitating effective and affordable treatments. This study aimed to develop marker-based quantification methods for Diospyros montana Roxb using DoE approach conduct in-silico anti-diabetic screening of its phytoconstituents. Methanolic extracts the plant underwent fractionation, with chloroform fraction used simultaneous HPLC Plumbagin Juglone. The in-vitro effects were evaluated through alpha-amylase alpha-glucosidase inhibition assays. Cytoscape 3.7.2 was construct networks linking phytoconstituents Type-2 diabetes targets pathways, while docking studies involved proteins 1SO2, 3H1V, 5DXU. A validated method quantified (Rt: 4.618) Juglone 3.998). showed significant enzyme IC50 values 36.775 33.124. Gene network analysis highlighted 8-hydroxyisodiospyrin, revealed Astragalin's strong binding 3H1V (score: −10.537). underscores potential in management, warranting further research.

Language: Английский

Development and validation of an LC-MS/MS method for the simultaneous estimation of tadalafil and macitentan in rat plasma: Greenness assessment and design of experiment approach DOI Creative Commons

Sravanthi Gandu,

Kumaraswamy Gandla, Lalitha Repudi

et al.

Green Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100211 - 100211

Published: Jan. 1, 2025

Language: Английский

Citations

0
Development of a Quality by Design-Based Ultra-Performance Liquid Chromatography Method for the Simultaneous Estimation of Casirivimab and Imdevimab with Greenness Metrics DOI Creative Commons

Sravanthi Gandu,

Kumaraswamy Gandla

Green Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100248 - 100248

Published: March 1, 2025

Language: Английский

Citations

0
Quality by design-based optimization and HP-TLC densitometric standardization of Theobroma cacao L. extract as a nutraceutical supplement DOI Creative Commons

Atith Muppayyanamath,

Darasaguppe R. Harish,

Vinayak Mastiholimath

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: April 9, 2025

Our previous studies identified the hydroalcoholic extract of defatted Theobroma cacao L. bean (CE) as a cancer-preventive and protective agent against chemotherapeutic-induced toxicities, specifically doxorubicin-induced heart, liver, kidney toxicities. An analytical method for phytochemical standardization was developed, acute oral toxicity studied in female Wistar rats following OECD 423 guidelines. In brief, CE extracted using an 80:20 alcohol-water (% v/v) mixture through cold maceration, followed by spray drying to obtain powdered CE. Utilizing Quality Design (QbD) approach with Expert (DoE) software, we optimized tablets via direct compression. The central composite design (CCD) included five center points, Avicel PH - 101 croscarmellose sodium (CCS) factors, disintegration time, hardness, % loss due friability measurements. Among 13 formulations, batch F-9 emerged one within space, containing 35% 5% CCS. formulation exhibited time 5.2 min, hardness 4.2 kg/cm2, 0.34%. Importantly, no toxic effects were found at 2,000 mg/kg study. contains vital bioactive polyphenols, including (-)-epigallocatechin-3-gallate (EGCG) (+)-catechin (CTN). We developed marker-based HP-TLC densitometric analysis mobile phase 9:9:2 v/v [ethyl acetate: toluene: formic acid], which revealed CTN Rf 0.49 EGCG 0.23. This validated according ICH requirements. conclusion, novel, simultaneously detects cocoa extract. Tablets formulated compression are safe nutraceuticals hold promise supplements palliative cancer therapy.

Language: Английский

Citations

0
Analytical Quality by Design (AQbD) Principles in Development and Validation of Stability‐Indicating RP‐HPLC Method for Simultaneous Estimation of Diltiazem HCl and Eugenol in Nanoethosomes DOI

Purva Usgaonkar,

Anjana Adhyapak,

Rahul Koli

et al.

Separation Science Plus, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 28, 2024

ABSTRACT The study developed and validated a robust reverse‐phase high‐performance liquid chromatography (RP‐HPLC) method for simultaneous estimation of Diltiazem HCl Eugenol in nanoethosomes using analytical quality by design (AQbD) principles. optimized utilized mobile phase methanol 0.1% Orthophosphoric acid (OPA) 50:50 % v/v ratio, with flow rate 1.0 mL/min isocratic elution on Phenomenex Luna C‐18 column (5 µm, 150 mm × 4.6 mm) at 30°C. This setup resulted retention times 6.838 min 23.135 Eugenol. Validation followed International Council Harmonization (ICH) Q2 (R1) guidelines, demonstrating excellent linearity correlation coefficients 0.9982 0.9991 across the 5–25 µg/mL range. limits detection (LOD) were 1.341 0.960 Eugenol, quantification (LOQ) 4.065 2.912 µg/mL, respectively. Stability testing under acidic, alkaline, oxidative, thermal, photolytic conditions adhered to ICH Q1A (R2) Q1B guidelines. AQbD‐based is effective routine analysis bulk pharmaceutical dosage forms, fully complying standards.

Language: Английский

Citations

3
Advanced stability-indicating gradient elution reverse phase-high performance liquid chromatography method development and validation for simultaneous estimation of rutin and curcumin using an analytical quality by design approach DOI

Daneshwari Bhusannavar,

Bhuvaneshwari R. Sharannavar,

Rahul Koli

et al.

Analytical Chemistry Letters, Journal Year: 2024, Volume and Issue: 14(4), P. 607 - 621

Published: July 3, 2024

AbstractThis research delves into a systematic Analytical Quality by Design (AQbD) approach to develop and validate stability-indicating robust RP-HPLC method for the simultaneous determination of Rutin Curcumin, compounds known their anti-inflammatory, antioxidant, wound-healing properties. Utilizing QbD principles, critical parameters (CMPs) were identified screened using design experiments (DoE) optimized via response surface methodology (RSM) with Central Composite (CCD). The chromatographic separation used Phenomenex Luna C18 column gradient elution, methanol 0.1% formic acid, flow rate 1 ml/min, detection at 345 nm (isosbestic point). retention times 5.63 mins 17.01 Curcumin. stability indication developed HPLC was performed, validation meticulously conducted following ICH Q2(R1) guidelines, demonstrating linearity over 2-10 μg/ml 5-25 correlation coefficients 0.9999 0.9998, respectively. Precision studies showed %RSD within acceptable limits, accuracies ranged between 97-100%. This validated offers reliable, sensitive quantifying suitable routine analysis in pharmaceutical laboratories quality control.GRAPHICAL ABSTRACTDisplay full sizeKeywords: designReverse-phase high-performance liquid chromatographyCentral composite designRutinCurcumin Supplementary informationFigures S1-S3 are given supplementary file.

Language: Английский

Citations

1
Secondary Metabolite Fingerprinting and Antioxidant Potency of Eleusine indica: An Integrated Chromatography and In silico Investigation DOI Creative Commons
Tapan Kumar Sahu, Nityananda Sahoo, Gurudutta Pattnaik

et al.

International Journal of experimental research and review, Journal Year: 2024, Volume and Issue: 40(Spl Volume), P. 245 - 255

Published: June 30, 2024

Natural regimens and constituents serve as the primary sources for drug conservation, each herbal regimen possesses unique ethnomedicinal biological properties. Therefore, researchers are currently more interested in exploring activity of existing plant species with most systematic approach to be used specific therapeutics proposed, either crude extracts or bioactive metabolites. The present study also explores antioxidant potency ethanolic Eleusine indica (a weed an agricultural field) through chemical fingerprinting analyses high-performance thin-layer chromatography (HPTLC), followed by gas chromatography-mass spectrometry (GCMS) silico investigation a way. Briefly, this aims observe presence secondary metabolites associated activity. After extraction, fingerprint was carried out mobile phase consisting ethyl acetate, acetic acid, formic water ratio 10:1.1:1.1:2.3 volume, where than eight fluorescent non-fluorescent bands were found, highest density peak area at Rf 0.87 lowest 0.18. As per GCMS report E. composed several Further, based on higher value, we have selected seven (EI_1 EI_7) that constitute molecular docking against three target enzymes: cyclooxygenase-2 (PDB ID: 5F1A), peroxiredoxin-5 1HD2), haemoxygenase 1 3CZY) assess potency. Overall, results revealed EI_7 (2,7-dioxatricyclo [4.4. 0.0(3,8)] decane-4,5-diol) showed similar ideal drug-ability profiles ascorbic acid considered lead further therapeutic use complementary agent.

Language: Английский

Citations

0
Stability‐Indicating RP‐HPLC Method for Lopinavir Estimation in Pharmaceutical Co‐Crystals: A Design of Expert Approach DOI

Pranali Mehta,

Bhuvaneshwari R. Sharannavar,

Rahul Koli

et al.

Separation Science Plus, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

ABSTRACT The primary objective of this investigation was to develop and validate a robust reversed‐phase high‐performance liquid chromatography (RP‐HPLC) method for the quantification lopinavir in co‐crystals formed with fumaric acid acetamide. A Box–Behnken design within experiment framework employed optimize chromatographic conditions. utilized Phenomenex Luna C18 column mobile phase methanol 0.1% formic (80:20 v/v) at flow rate 1 mL/min, detection 259 nm. Validation followed ICH Q2 (R1 R2) guidelines, confirming method's specificity, accuracy, precision, linearity, robustness. Co‐crystals were prepared using liquid‐assisted grinding reaction crystallization techniques. validated HPLC successfully quantified these co‐crystals, recovery rates ranging from 96.3% 99.8%. Forced degradation studies under various stress conditions (acidic, base, oxidative, thermal, photolytic) demonstrated capability distinguish its products. In conclusion, developed RP‐HPLC is suitable routine analysis quality control co‐crystal formulations. This not only provides precise but also offers insights into enhanced delivery potential through co‐crystallization, contributing improved therapeutic efficacy HIV/AIDS treatment.

Language: Английский

Citations

0
Development of marker-based quantification methods fo r Diospyros Montana Roxb using DoE approach and in-silico anti-diabetic screening of selected phytoconstituents of the Diospyros genus DOI

Ashwini M. Kambalyal,

Parixit Bhandurge,

Nikhil Gawas

et al.

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: Dec. 26, 2024

Diabetes mellitus is a rising global health issue, necessitating effective and affordable treatments. This study aimed to develop marker-based quantification methods for Diospyros montana Roxb using DoE approach conduct in-silico anti-diabetic screening of its phytoconstituents. Methanolic extracts the plant underwent fractionation, with chloroform fraction used simultaneous HPLC Plumbagin Juglone. The in-vitro effects were evaluated through alpha-amylase alpha-glucosidase inhibition assays. Cytoscape 3.7.2 was construct networks linking phytoconstituents Type-2 diabetes targets pathways, while docking studies involved proteins 1SO2, 3H1V, 5DXU. A validated method quantified (Rt: 4.618) Juglone 3.998). showed significant enzyme IC50 values 36.775 33.124. Gene network analysis highlighted 8-hydroxyisodiospyrin, revealed Astragalin's strong binding 3H1V (score: −10.537). underscores potential in management, warranting further research.

Language: Английский

Citations

0